Current Knowledge and Future Directions in Developing Strategies to Combat Pseudomonas aeruginosa Infection

Dolan, Stephen K.

doi:10.1016/j.jmb.2020.07.021

Cited by 31 publications

(37 citation statements)

References 161 publications

(191 reference statements)

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“…Metabolic flexibility of P. aeruginosa could lead to new strategies to combat bacterial infection ( 24 ). The present study explored metabolic mechanisms of SCF resistance in P. aeruginosa evolved in vivo and in vitro .…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that many of the key adaptations that arise in P. aeruginosa during infection are centered on the core metabolism ( 24 ). The metabolomes of wild-type P. aeruginosa strain K (polymyxin B MIC, 1 mg/liter) and its paired pmrB mutant strains, PAKpmrB6 and PAKpmrB12 (polymyxin B MICs of 16 mg/liter and 64 mg/liter, respectively), show that the decreased phospholipid level is associated with polymyxin resistance ( 19 ).…”

Section: Discussionmentioning

confidence: 99%

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Inactivation of Nitrite-Dependent Nitric Oxide Biosynthesis Is Responsible for Overlapped Antibiotic Resistance between Naturally and Artificially Evolved Pseudomonas aeruginosa

et al. 2021

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Inactivation of Nitrite-Dependent Nitric Oxide Biosynthesis Is Responsible for Overlapped Antibiotic Resistance between Naturally and Artificially Evolved Pseudomonas aeruginosa

et al. 2021

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“…Pseudomonas aeruginosa is a multidrug-resistant gram-negative opportunistic bacterium that can be found in diverse niches from plants, animals, and humans, also causing severe infections, especially in ventilated patients [19]. Due to its intrinsic resistance and the great capacity to additionally acquire resistance genes by horizontal transfer, P. aeruginosa infections are very difficult to treat [20,21].…”

Section: Introductionmentioning

confidence: 99%

Eugenol-Functionalized Magnetite Nanoparticles Modulate Virulence and Persistence in Pseudomonas aeruginosa Clinical Strains

et al. 2021

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Efficient antibiotics to cure Pseudomonas aeruginosa persistent infections are currently insufficient and alternative options are needed. A promising lead is to design therapeutics able to modulate key phenotypes in microbial virulence and thus control the progression of the infectious process without selecting resistant mutants. In this study, we developed a nanostructured system based on Fe3O4 nanoparticles (NPs) and eugenol, a natural plant-compound which has been previously shown to interfere with microbial virulence when utilized in subinhibitory concentrations. The obtained functional NPs are crystalline, with a spherical shape and 10–15 nm in size. The subinhibitory concentrations (MIC 1/2) of the eugenol embedded magnetite NPs (Fe3O4@EUG) modulate key virulence phenotypes, such as attachment, biofilm formation, persister selection by ciprofloxacin, and the production of soluble enzymes. To our knowledge, this is the first report on the ability of functional magnetite NPs to modulate P. aeruginosa virulence and phenotypic resistance; our data highlights the potential of these bioactive nanostructures to be used as anti-pathogenic agents.

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“…The large genome of P. aeruginosa provides it with metabolic versatility and an array of pathogenic mechanisms. It causes infection of burn wounds, often resulting in invasive infections sepsis and death, while respiratory infection is the leading reason of morbidity in cystic fibrosis patients [Dolan, 2020]. P. aeruginosa PA14 is a clinical isolate obtained originally from a burn patient, which displays pathogenicity in a variety of genetically tractable model hosts and mice [Mahajan-Miklos et al, 2000].…”

Section: Unifying Principlesmentioning

confidence: 99%

The Genetics of Prey Susceptibility to Myxobacterial Predation: A Review, Including an Investigation into Pseudomonas aeruginosa Mutations Affecting Predation by Myxococcus xanthus

Sydney

Swain

et al. 2021

Microb Physiol

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Bacterial predation is a ubiquitous and fundamental biological process, which influences the community composition of microbial ecosystems. Among the best characterised bacterial predators are the myxobacteria, which include the model organism Myxococcus xanthus. Predation by M. xanthus involves the secretion of antibiotic metabolites and hydrolytic enzymes, which results in the lysis of prey organisms and release of prey nutrients into the extracellular milieu. Due to the generalist nature of this predatory mechanism, M. xanthus has a broad prey range, being able to kill and consume Gram-negative/positive bacteria and fungi. Potential prey organisms have evolved a range of behaviours which protect themselves from attack by predators. In recent years, several investigations have studied the molecular responses of a broad variety of prey organisms to M. xanthus predation. It seems that the diverse mechanisms employed by prey belong to a much smaller number of general “predation resistance” strategies. In this mini-review, we present the current state of knowledge regarding M. xanthus predation, and how prey organisms resist predation. As previous molecular studies of prey susceptibility have focussed on individual genes/metabolites, we have also undertaken a genome-wide screen for genes of Pseudomonas aeruginosa which contribute to its ability to resist predation. P. aeruginosa is a World Health Organisation priority 1 antibiotic-resistant pathogen. It is metabolically versatile and has an array of pathogenic mechanisms, leading to its prevalence as an opportunistic pathogen. Using a library of nearly 5,500 defined transposon insertion mutants, we screened for “prey genes”, which when mutated allowed increased predation by a fluorescent strain of M. xanthus. A set of candidate “prey proteins” were identified, which shared common functional roles and whose nature suggested that predation resistance by P. aeruginosa requires an effective metal/oxidative stress system, an intact motility system, and mechanisms for de-toxifying antimicrobial peptides.

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Current Knowledge and Future Directions in Developing Strategies to Combat Pseudomonas aeruginosa Infection

Cited by 31 publications

References 161 publications

Inactivation of Nitrite-Dependent Nitric Oxide Biosynthesis Is Responsible for Overlapped Antibiotic Resistance between Naturally and Artificially Evolved Pseudomonas aeruginosa

Inactivation of Nitrite-Dependent Nitric Oxide Biosynthesis Is Responsible for Overlapped Antibiotic Resistance between Naturally and Artificially Evolved Pseudomonas aeruginosa

Eugenol-Functionalized Magnetite Nanoparticles Modulate Virulence and Persistence in Pseudomonas aeruginosa Clinical Strains

The Genetics of Prey Susceptibility to Myxobacterial Predation: A Review, Including an Investigation into <b><i>Pseudomonas aeruginosa</i></b> Mutations Affecting Predation by <b><i>Myxococcus xanthus</i></b>

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