Current issues and future perspectives of gastric cancer screening

Hamashima, Chisato

doi:10.3748/wjg.v20.i38.13767

Cited by 149 publications

(144 citation statements)

References 34 publications

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“…Gastric cancer (GC) ranks as the fourth most common type of malignant tumor in morbidity and the second leading cause of cancer death in the world [1,2]. Although treatment strategies have substantially developed over the years, the prognosis for GC remains to be improved [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Exosomal miR-27a Derived from Gastric Cancer Cells Regulates the Transformation of Fibroblasts into Cancer-Associated Fibroblasts

Wang

Guan

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: The malignant biological behavior of gastric cancer(GC) is not only determined by cancer cells alone, but also closely regulated by the microenvironment. Fibroblasts represent a large proportion of the components in the tumor microenvironment, and they promote the development of disease. Currently, accumulating evidence suggests that exosomes can function as intercellular transport systems to relay their contents, especially microRNAs(miRNAs). Methods: First, we detected the highly-expressed level of miR-27a in exosomes isolated from gastric cancer cells by qRT-PCR. MiR-27a –over-expressed models in vitro and in vivo were established to investigate the transformation of cancer-associated fibroblasts observed by Western blotting, and the malignant behavior of gastric cancer cells using the methods CCK8 and Transwell. Moreover, the downregulation of CSRP2 in fibroblasts was used to evaluate the promotion of malignancy of gastric cancer using the methods CCK8 and Transwell. Results: In this study, we found a marked high level of miR-27a in exosomes derived from GC cells. miR-27a was found to function an oncogene that not only induced the reprogramming of fibroblasts into cancer-associated fibroblasts(CAFs), but also promoted the proliferation, motility and metastasis of cancer cells in vitro and in vivo. Conversely, CAFs with over-expression of miR-27a could pleiotropically increase the malignant behavior of the GC cells. For the first time, we revealed that CSRP2 is a downstream target of miR-27a. CSRP2 downregulation could increase the proliferation and motility of GC cells. Conclusion: Thus, this report indicates that miR-27a in exosomes derived from GC cells has a crucial impact on the microenvironment and may be used as a potential therapeutic target in the treatment of GC

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Section: Introductionmentioning

confidence: 99%

Exosomal miR-27a Derived from Gastric Cancer Cells Regulates the Transformation of Fibroblasts into Cancer-Associated Fibroblasts

Wang

Guan

Zhang

et al. 2018

Cell Physiol Biochem

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“…Over the last 4 decades its incidence has decreased globally; however, higher rates of mortality have been observed in East Asian countries than in other areas of the world (16). Genetic and environmental factors may serve a role in the etiology of gastric cancer (17,18), and efforts are being made to further understand and treat this disease.…”

Section: Introductionmentioning

confidence: 99%

“…Gastric cancer is one of the most common types of cancer in Asia (15,16). Over the last 4 decades its incidence has decreased globally; however, higher rates of mortality have been observed in East Asian countries than in other areas of the world (16).…”

Section: Introductionmentioning

confidence: 99%

Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells

Yuan

Zhang

et al. 2018

Oncol Lett

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Abstract. The objective of the present study was to investigate the cytotoxic effects of sodium citrate on human gastric adenocarcinoma epithelia AGS cells. Numerous cytotoxicity-associated sodium citrate-induced effects were assessed, including cell viability and proliferation, cytokine expression and caspase activity. In vitro studies demonstrated that incubation with sodium citrate (>3.125 mM) inhibited AGS cell viability and proliferation in a dose-dependent manner. Incubation with sodium citrate for 24 h revealed that the levels of interleukin-1β (IL-1β), IL-8 and tumor necrosis factor increased with an increasing of dose of sodium citrate, whereas the IL-6 levels exhibited only a slight alteration. In addition, increases in caspase-3 and -9 activities were associated with increased duration of treatment and dosage of sodium citrate. Collectively, the results of the present study demonstrated that treatment with sodium citrate at higher concentrations or for longer durations exerts a cytotoxic effect on AGS cells via the induction of the intrinsic apoptosis pathway and the alteration in the levels of certain cytokines.

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“…Gastric cancer is currently the third leading cause of cancer death worldwide, it used to be the second cause after lung cancer and remains a major public health problem in the world [10]- [12]. Although the incidence of gastric cancer has decreased around the world over the last 3 decades, which still show a higher mortality than other countries in East Asian countries [11]. Cure is possible for patients presenting with early stage disease [10].…”

Section: Introductionmentioning

confidence: 99%

“…However, because early gastric cancer produces few symptoms, most gastric cancer patients are diagnosed in advanced-stage or metastatic disease with a poor prognosis [10]. Radiographic screening and endoscopic screening are common technologies to detect early-stage gastric cancer [11].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Sodium Nitrite on Induction of Apoptosis in Human Gastric Adenocarcinoma Epithelia (AGS) Cells

Liu¹,

Qi-liang²,

Bo³

et al. 2015

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To examine the cytocidal effect of sodium nitrite on the cancer cell, we subjected human gastric adenocarcinoma epithelia (AGS) cells to various experimentation following exposure to sodium nitrite, and measured the resulting changes in the levels of cell death, lactate dehydrogenase (LDH) release, and caspase-3, -6, -8, and -9 activities. Our data revealed that, in AGS cells, treatment with ≥6.25 mM sodium nitrite for 8 h resulted in an obvious increase in cell death. LDH release was also markedly increased following sodium nitrite treatment, but at a concentration of ≥6.25 mM for 24 h. This increasing trend showed a positive correlation (r = 0.9564, P < 0.05). In addition, we detected pronounced increases in caspase activities with various concentrations of sodium nitrite: caspase-3 at ≥25 mM for 1 h, ≥12.5 mM for 3 h and 6 h; caspase-9 at 50 mM for 1 h and 3 h, and ≥6.25 mM for 6 h; and caspase-6 at 50 mM for 1 h and 3 h. We did not however, detect any observable increase in the activity of caspase-8 following sodium nitrite treatment at any concentration or for any duration of treatment in this study. This data demonstrates that, in AGS cells, higher concentrations or longer durations of treatment with sodium nitrite could exhibit a cytocidal effect, and that sodium nitrite could induce apoptosis via activation of the caspase-9, caspase-3 cascade (intrinsic pathway) and caspase-6.

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Current issues and future perspectives of gastric cancer screening

Cited by 149 publications

References 34 publications

Exosomal miR-27a Derived from Gastric Cancer Cells Regulates the Transformation of Fibroblasts into Cancer-Associated Fibroblasts

Exosomal miR-27a Derived from Gastric Cancer Cells Regulates the Transformation of Fibroblasts into Cancer-Associated Fibroblasts

Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells

The Effect of Sodium Nitrite on Induction of Apoptosis in Human Gastric Adenocarcinoma Epithelia (AGS) Cells

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