Current Insights into Collagen Type I

Abstract

Collagen type I (Col-I) is unique due to its high biocompatibility in human tissue. Despite its availability from various sources, Col-I naturally mimics the extracellular matrix (ECM) and generally makes up the larger protein component (90%) in vasculature, skin, tendon bone, and other tissue. The acceptable physicochemical properties of native Col-I further enhance the incorporation of Col-I in various fields, including pharmaceutical, cosmeceutical, regenerative medicine, and clinical. This review aims to discuss Col-I, covering the structure, various sources of availability, native collagen synthesis, current extraction methods, physicochemical characteristics, applications in various fields, and biomarkers. The review is intended to provide specific information on Col-I currently available, going back five years. This is expected to provide a helping hand for researchers who are concerned about any development on collagen-based products particularly for therapeutic fields.

Development of a Hybrid Polymer-Based Microfluidic Platform for Culturing Hepatocytes towards Liver-on-a-Chip Applications

“…In polycarbonate and COC, better cell adherence and growth were observed when the substrates were preliminarily coated with collagen than their bare counterparts. Collagen is a type of triple-helix structure protein that can initiate and maintain the interaction between cells and the matrix [ 26 ]. Collagen type I, the most common type of protein [ 26 ], has been used in this work.…”

“…Collagen is a type of triple-helix structure protein that can initiate and maintain the interaction between cells and the matrix [ 26 ]. Collagen type I, the most common type of protein [ 26 ], has been used in this work. In PDMS, coating it with collagen was not considered, as the PDMS is used only as a top layer of microfluidic devices, and the design does not require cell placement on this material.…”

“…Cyclic olefin copolymer (COC) was considered a potential material for microfluidic devices in this work due to its applicability for rapid prototyping similar to PC, PDMS, and its high melting temperature suitable for autoclaving for cell culture applications, and many other advantages [ 4 ]. Since COC is used as the bottom part of the COC–PDMS ( Figure 1 b) chip, COC substrates were coated with collagen due to the favorable advantages of collagen to cell culture [ 26 ]. Microscopy imaging of cell growth on collagen-coated COC slabs ( Figure S3 ) and the collagen-coated chip microchannel in the lower part of the HCP chip ( Figure 2 a) confirmed the biocompatible nature of the COC substrate as expected and as previously reported in the literature [ 7 ].…”

Zebrafish as a Model System to Study the Mechanism of Cutaneous Wound Healing and Drug Discovery: Advantages and Challenges

“…In these, Panax ginseng extract contains bioactive compounds such as ginsenoside Rg1, which may exhibit anti-inflammatory and anti-oxidant properties at the wounded region [52,53]. Such properties have proven to enhance healing by increasing the rate of collagen synthesis at the injury site [54]. In the study by He et al (2020), they investigated the anti-inflammatory effects of Rg1 using a tail fin amputation of the Zebrafish larva [53].…”

Stiffness Variation of 3D Collagen Networks by Surface Functionalization of Network Fibrils with Sulfonated Polymers

“…Collagen I (Coll I) is one of the most prominent proteins of the extracellular matrix in mammalian tissues. It constitutes fibrillar networks and tendons as a very important structural component [ 1 ]. Because of this, and originating from easily applicable reconstitution approaches for fibrillar Coll I networks from rat or bovine Coll I, 3D fibrillar Coll I networks are a widely distributed gold standard for biomimetic 3D cell culture scaffolds [ 2 , 3 , 4 ].…”