Current Developments in µMAS NMR Analysis for Metabolomics

Lucas‐Torres, Covadonga; Wong, Alan

doi:10.3390/metabo9020029

Cited by 13 publications

(18 citation statements)

References 46 publications

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“…Many integrated techniques of NMR have been applied to identify the structural units of unknown metabolites. Recently developed advanced tools for plant metabolomics are one-and two-dimensional NMR [28], isotope-labeled NMR [29], and micro-coil NMR [30]. NMR is the only tool that can detect the specific labelling of stable isotopes [3].…”

Section: Advanced Tools For Analytical Research In Plant Metabolomicsmentioning

confidence: 99%

Metabolomics: A Way Forward for Crop Improvement

et al. 2019

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Metabolomics is an emerging branch of “omics” and it involves identification and quantification of metabolites and chemical footprints of cellular regulatory processes in different biological species. The metabolome is the total metabolite pool in an organism, which can be measured to characterize genetic or environmental variations. Metabolomics plays a significant role in exploring environment–gene interactions, mutant characterization, phenotyping, identification of biomarkers, and drug discovery. Metabolomics is a promising approach to decipher various metabolic networks that are linked with biotic and abiotic stress tolerance in plants. In this context, metabolomics-assisted breeding enables efficient screening for yield and stress tolerance of crops at the metabolic level. Advanced metabolomics analytical tools, like non-destructive nuclear magnetic resonance spectroscopy (NMR), liquid chromatography mass-spectroscopy (LC-MS), gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), and direct flow injection (DFI) mass spectrometry, have sped up metabolic profiling. Presently, integrating metabolomics with post-genomics tools has enabled efficient dissection of genetic and phenotypic association in crop plants. This review provides insight into the state-of-the-art plant metabolomics tools for crop improvement. Here, we describe the workflow of plant metabolomics research focusing on the elucidation of biotic and abiotic stress tolerance mechanisms in plants. Furthermore, the potential of metabolomics-assisted breeding for crop improvement and its future applications in speed breeding are also discussed. Mention has also been made of possible bottlenecks and future prospects of plant metabolomics.

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Section: Advanced Tools For Analytical Research In Plant Metabolomicsmentioning

confidence: 99%

Metabolomics: A Way Forward for Crop Improvement

et al. 2019

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“…Due to the small sizes of specific structures of plants, high-resolution micro-MAS probe (HR-μMAS) can be considered. A lot smaller sample size (<0.5 mg) can be used in HR-μMAS in comparison to standard HR-MAS sample size (~100–150 mg) [ 84 ]. This can be used to study specific parts of plant, as shown for garlic [ 85 ].…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

HR-MAS NMR Applications in Plant Metabolomics

2021

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Metabolomics is used to reduce the complexity of plants and to understand the underlying pathways of the plant phenotype. The metabolic profile of plants can be obtained by mass spectrometry or liquid-state NMR. The extraction of metabolites from the sample is necessary for both techniques to obtain the metabolic profile. This extraction step can be eliminated by making use of high-resolution magic angle spinning (HR-MAS) NMR. In this review, an HR-MAS NMR-based workflow is described in more detail, including used pulse sequences in metabolomics. The pre-processing steps of one-dimensional HR-MAS NMR spectra are presented, including spectral alignment, baseline correction, bucketing, normalisation and scaling procedures. We also highlight some of the models which can be used to perform multivariate analysis on the HR-MAS NMR spectra. Finally, applications of HR-MAS NMR in plant metabolomics are described and show that HR-MAS NMR is a powerful tool for plant metabolomics studies.

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“…These findings on MACS inserts for metabolomics have been comprehensively summarized recently by Lucas-Torres and Wong. 21 Although metabolomic studies have been performed on tissue samples, yeast cells and C. elegans worms, the potential Fig. 4 Comparison of NMR spectrum of 500 mM sucrose obtained from a MACS and a dummy MACS insert acquired from a volume of 380 nl.…”

Section: Metabolomicsmentioning

confidence: 99%