Amino acids, being the primary products of inorganic assimilation and precursors of proteins, play an essential role in plant metabolism. The objective of this study was to compare guar plants which were grown under salt-stress conditions (150 mM NaCl), in terms of the effect of different amino acid treatments (300, 600, 1200, and 1800 mg L -1 amino acid) on physiological, morphological, and enzymatic activity. Amino acid (AA) applications significantly increased fresh and dry weight, relative water content, photosynthetic pigments, total phenolic, flavonoid and free amino acid contents, K + and Ca ++ ion concentration, ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD) activity of guar plants compared to the salt stress treatment the ones under no AA treatment. According to the results, the malondialdehyde (MDA) content, Na + and Clion contents were significantly decreased. Hence, the results support the application of the amino acid in 600 mg L -1 doses in order to increase the defense system of the guar plant, enabling it to tolerate the negative effects induced by salinity.