Current Clinical Status of the Extracorporeal Liver Support Devices

Baquerizo, Angeles; Bañares, Rafael; Saliba, Faouzi

doi:10.1016/b978-1-4557-0268-8.00107-x

Cited by 5 publications

(13 citation statements)

References 195 publications

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“…Overall, HepG2 cells produce less albumin and AFP and show lower cytochrome P-450 activity than C3A cells. 97 In addition, HepG2 cells exhibit 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase (ATCC) activities.…”

Section: Dovepressmentioning

confidence: 99%

Diamond Nanofilm Normalizes Proliferation and Metabolism in Liver Cancer Cells

Sosnowska¹,

Kutwin²,

Strojny³

et al. 2021

NSA

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Surgical resection of hepatocellular carcinoma can be associated with recurrence resulting from the degeneration of residual volume of the liver. The objective was to assess the possibility of using a biocompatible nanofilm, made of a colloid of diamond nanoparticles (nfND), to fill the side after tumour resection and optimize its contact with proliferating liver cells, minimizing their cancerous transformation. Methods: HepG2 and C3A liver cancer cells and HS-5 non-cancer cells were used. An aqueous colloid of diamond nanoparticles, which covered the cell culture plate, was used to create the nanofilm. The roughness of the resulting nanofilm was measured by atomic force microscopy. Mitochondrial activity and cell proliferation were measured by XTT and BrdU assays. Cell morphology and a scratch test were used to evaluate the invasiveness of cells. Flow cytometry determined the number of cells within the cell cycle. Protein expression in was measured by mass spectrometry. Results: The nfND created a surface with increased roughness and exposed oxygen groups compared with a standard plate. All cell lines were prone to settling on the nanofilm, but cancer cells formed more relaxed clusters. The surface compatibility was dependent on the cell type and decreased in the order C3A >HepG2 >HS-5. The invasion was reduced in cancer lines with the greatest effect on the C3A line, reducing proliferation and increasing the G2/M cell population. Among the proteins with altered expression, membrane and nuclear proteins dominated. Conclusion:In vitro studies demonstrated the antiproliferative properties of nfND against C3A liver cancer cells. At the same time, the need to personalize potential therapy was indicated due to the differential protein synthetic responses in C3A vs HepG2 cells. We documented that nfND is a source of signals capable of normalizing the expression of many intracellular proteins involved in the transformation to non-cancerous cells.

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Section: Dovepressmentioning

confidence: 99%

Diamond Nanofilm Normalizes Proliferation and Metabolism in Liver Cancer Cells

Sosnowska¹,

Kutwin²,

Strojny³

et al. 2021

NSA

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“…These cells have the advantage of unlimited availability but show less liver-specific metabolism compared with normal liver cells. 56,57 Human SCs could also serve as a source of hepatocytelike cells, but, to date, fully metabolically mature hepatocyte-like cells for transplantation have been elusive. 58 Other hurdles include determining the optimal engraftment site and allo-immunity.…”

Section: H E Pato C Y Te S: E X Pe R I M E Ntati O N and Clinic Al Opp Ortunit Ymentioning

confidence: 99%

“…To further address the scarcity of PHHs, several liver cell lines have been developed by gene transfer from human liver tumors or from immortalized human hepatocytes. These cells have the advantage of unlimited availability but show less liver‐specific metabolism compared with normal liver cells 56,57 . Human SCs could also serve as a source of hepatocyte‐like cells, but, to date, fully metabolically mature hepatocyte‐like cells for transplantation have been elusive 58 .…”

Section: Hepatocytes: Experimentation and Clinical Opportunitymentioning

confidence: 99%

“…48,55 The bioartificial liver (BAL), an application of hepatocyte transplantation, utilizes viable animal or human hepatocytes loaded into bioreactors 63 as the blood or plasma of a liver failure patient are perfused to provide synthetic function (proteins and clotting factors), regulatory (hormones), detoxification, and liver-based immunity. 57 A shortage of adequate and efficient PHHs has been a primary challenge in BAL development. 56,57,64,65 An alternative hepatocyte sources for the BAL are xenogeneic cells, such as porcine hepatocytes.…”

Section: H E Pato C Y Te S: E X Pe R I M E Ntati O N and Clinic Al Opp Ortunit Ymentioning

confidence: 99%

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Challenges, highlights, and opportunities in cellular transplantation: A white paper of the current landscape

Parsons

Baquerizo

Kirchner

et al. 2021

American Journal of Transplantation

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Although cellular transplantation remains a relatively small field compared to solid organ transplantation, the prospects for advancement in basic science and clinical care remain bountiful. In this review, notable historical events and the current landscape of the field of cellular transplantation are reviewed with an emphasis on islets (allo-and xeno-), hepatocytes (including bioartificial liver), adoptive regulatory immunotherapy, and stem cells (SCs, specifically endogenous organ-specific and mesenchymal). Also, the nascent but rapidly evolving field of three-dimensional bioprinting is highlighted, including its major processing steps and latest achievements. To reach its full potential where cellular transplants are a more viable alternative than solid organ transplants, fundamental change in how the field is regulated and advanced is needed. Greater public and private investment in the development of cellular transplantation is required. Furthermore, consistent with the call of multiple national transplant societies for allo-islet transplants, the oversight of cellular transplants should mirror that of solid organ transplants and not be classified under the unsustainable, outdated model | 3227 AJT PARSONS et Al.

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“…Therefore, the chalcones 3a – f were applied in imaging of normal cells (human embryonic kidney 293, HEK-293) and cancer cells (human hepatocellular carcinoma, HepG2). Both cell lines are commonly used in research because they are easy to handle and suitable for various types of assays [ 36 , 37 ]. Moreover, the antimicrobial properties of 3a – f were also tested against a Gram-negative bacterium ( Escherichia coli ) and a Gram-positive bacterium ( Staphylococcus aureus ), which are common members of the human microbiota but can act as opportunistic pathogens and cause disease.…”

Section: Introductionmentioning

confidence: 99%

Photophysical Study and Biological Applications of Synthetic Chalcone-Based Fluorescent Dyes

et al. 2021

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A chalcone series (3a–f) with electron push–pull effect was synthesized via a one-pot Claisen–Schmidt reaction with a simple purification step. The compounds exhibited strong emission, peaking around 512–567 nm with mega-stokes shift (∆λ = 93–139 nm) in polar solvents (DMSO, MeOH, and PBS) and showed good photo-stability. Therefore, 3a–f were applied in cellular imaging. After 3 h of incubation, green fluorescence was clearly brighter in cancer cells (HepG2) compared to normal cells (HEK-293), suggesting preferential accumulation in cancer cells. Moreover, all compounds exhibited higher cytotoxicity within 24 h toward cancer cells (IC50 values ranging from 45 to 100 μM) than normal cells (IC50 value >100 μM). Furthermore, the antimicrobial properties of chalcones 3a–f were investigated. Interestingly, 3a–f exhibited antibacterial activities against Escherichia coli and Staphylococcus aureus, with minimum bactericidal concentrations (MBC) of 0.10–0.60 mg/mL (375–1000 µM), suggesting their potential antibacterial activity against both Gram-negative and Gram-positive bacteria. Thus, this series of chalcone-derived fluorescent dyes with facile synthesis shows great potential for the development of antibiotics and cancer cell staining agents.

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Current Clinical Status of the Extracorporeal Liver Support Devices

Cited by 5 publications

References 195 publications

Diamond Nanofilm Normalizes Proliferation and Metabolism in Liver Cancer Cells

Diamond Nanofilm Normalizes Proliferation and Metabolism in Liver Cancer Cells

Challenges, highlights, and opportunities in cellular transplantation: A white paper of the current landscape

Photophysical Study and Biological Applications of Synthetic Chalcone-Based Fluorescent Dyes

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