Current challenges and future prospects in chromatographic method development for pharmaceutical research

High throughput analysis enables high throughput experimentation in pharmaceutical process research

“…Since a major focus of our effort was developing rapid solutions to problems involving chirality and stereochemistry, one of the first platforms created was a rapid analytical method development capability for the chromatographic separation of enantiomers using SFC, which afforded researchers a 'same day' or at worst 'next day' ability to measure the enantiopurity of newly synthesized compounds. This platform has evolved both in terms of speed and universality over the ensuing years, 26,27 but still provides an important foundation for many other experimental platforms (Fig. 3).…”

Voltammetry as the First Method for Direct Determination of a Novel Antagonist of A2A Adenosine Receptors

“…The most popular method for the determination of bioactive compounds in biological fluids and pharmaceutical formulations is chromatography, mainly liquid chromatography with different types of detectors . Although chromatographic methods belong to one of the most selective and sensitive techniques, they have some disadvantages, e. g. a time consuming analysis, an expensive instrumentation and a large consumption of organic solvents.…”

“…Although chromatographic methods belong to one of the most selective and sensitive techniques, they have some disadvantages, e. g. a time consuming analysis, an expensive instrumentation and a large consumption of organic solvents. Additionally, the pretreatment procedure (e. g. derivatization, extraction process) is required in the analysis of complex matrices such as human urine, blood and serum . Electrochemical methods, including voltammetry, are characterized by the high selectivity, sensitivity and a low cost of equipment.…”

Signal enhancement in supercritical fluid chromatography‐diode‐array detection with multiple injection

“…Major application fields of SFC include pharmaceutical analysis, biological sample analysis, natural and food product analysis . Revolutionary development in modern SFC instrumentation has also brought dramatic improvement in noise reduction, which opens up the possibility for trace analysis of environmental and biological samples . For such analysis, SFC methods with enhanced analyte detectability are in most cases needed, enabling for lower detection limits.…”