Current Approach to Hepatocellular Carcinoma

Abrams, Peter; Marsh, J. Wallis

doi:10.1016/j.suc.2010.04.010

Cited by 52 publications

(37 citation statements)

References 64 publications

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“…Primary liver malignancies such as hepatocellular carcinoma and cholangiocarcinoma together with metastatic liver tumors from other organs are among the most common tumors in human. The best available treatment option for these diseases is surgical resection [1]. The median 5-year survival rate after liver resection reaches 30–50% if R0 is achieved, and in cases of R1/2 the survival decreases to a rate of 7% [2].…”

Section: Introductionmentioning

confidence: 99%

Hypothermic ante situm Resection in Tumors of the Hepatocaval Confluence

et al. 2011

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Primary liver malignancies together with metastatic liver tumors are among the most common tumors in human. The best available treatment option for these diseases is surgical resection. One major parameter which had been considered as contraindication for liver resection owing to technical difficulties in achieving tumor-free margins was the involvement of the hepatocaval confluence. To overcome this problem, several techniques including hypothermic in situ, ante situm and ex situ liver resection have been introduced. The common basis for these liver resections is the total vascular exclusion of the liver, and perfusion of the organ by preservation solution under hypothermic conditions. The major indications for the ante situm liver resection are tumors in the liver that are either unresectable or inadequately resectable by conventional surgery because they involve the venous confluence and/or the retrohepatic vena cava, or are in close proximity to them. This technique is a realistic option to achieve surgical cure or substantial gain of life time with quality of life in otherwise unresectable tumors of the liver. Due to fewer anastomoses, the ante situm approach is easier and safer than the ex situ approach, with an acceptable morbidity and mortality rate. Patient selection is of utmost importance to achieve a good outcome. To minimize the postoperative morbidities and mortality, this procedure requires a multidisciplinary approach and should be performed in experienced centers with a high case volume of hepatobiliary surgeries.

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Section: Introductionmentioning

confidence: 99%

Hypothermic ante situm Resection in Tumors of the Hepatocaval Confluence

et al. 2011

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“…Globally, HCC is ranked as the fourth leading cause of cancer-related fatalities (1). Patients with chronic liver diseases associated with hepatitis B virus or hepatitis C virus infections frequently develop HCC (2).…”

Section: Introductionmentioning

confidence: 99%

Mechanism of apoptosis induction in human hepatocellular carcinoma cells following treatment with a gecko peptides mixture

Jin

Duan

et al. 2016

Biomedical Reports

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Abstract. The aim of the present study was to investigate the apoptotic effect and molecular mechanisms of gecko peptides mixture (GPM) on the human liver carcinoma HepG2 cell line in vitro. The methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was performed to identify the dose-(0.10, 0.15, 0.20, 0.25 and 0.30 mg/ml) and time-dependent (24, 48 and 72 h) inhibitory effect of GPM on HepG2 cells and their proliferation. Hoechst 33258 staining was carried out to detect the nuclear change coupled with apoptosis induced by GPM. Western blotting was used to evaluate apoptosis-related protein expression changes induced by GPM, including caspase, cytochrome c (Cyt c) and apoptosis-inducing factor (AIF). MTT results showed that GPM significantly inhibited the proliferation of HepG2 cells in a dose-and time-dependent manner. Hoechst 33258 staining demonstrated that GPM induced typical apoptotic morphological changes, while western blotting analysis revealed that GPM increased caspase-3, caspase-9, Cyt c and AIF protein expression levels in HepG2 cells treated with 0.06 or 0.08 mg/ml for 24 h. In conclusion, GPM could induce apoptosis by activating the intrinsic mitochondrial apoptotic pathways.

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“… A better understanding of hepatocellular carcinoma (HCC)-the most common primary liver cancer, which ranks globally as the third or fourth leading cause of cancer-related death [1,2], is imperative for improving early diagnosis and subsequent patient treatment and prognosis. Approximately 700,000 new cases are diagnosed each year [3].…”

Section: Introductionmentioning

confidence: 99%

Mechanism Analyses for Elucidating the Role of LOXL2 Silencing in Hepatocellular Carcinoma

Wu¹,

Zhang²,

Zhu³

et al. 2015

JAST-A

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Abstract:The paper aimed to study the effect of lysyl oxidase-like 2 (LOXL2) on hepatocellular carcinoma (HCC) and explore the biological mechanisms of tumorigenicity and progression in HCC. The authors used four HCC cell lines to identify LOXL2. A lentiviral vector containing LOXL2-siRNA was constructed to silence the LOXL2 gene in SMMC-7721 cell line, and mRNA of the target gene was detected by real-time polymerase chain reaction (RT-PCR). The effect of LOXL2 silencing on the growth of SMMC-7721 cells was explored with flow cytometry profiling and BrdU labeling. Downstream genes of LOXL2 were selected by microarray and verified by Western Blotting. In the results, LOXL2 expression was significantly up-regulated in four types of HCC cell lines, therefore, SMMC-7721 cell line was selected for further exploration. When SMMC-7721 cell line was infected with LOXL2-siRNA, the expression of LOXL2 mRNA decreased. The silencing of LOXL2 resulted in the cell cycle arrest at the G1-phase, the increased apoptosis and the decreased growth of SMMC-7721 cells on the indicated days by BrdU. Moreover, the MDM2, BIRC3, CDC42, FOS and TGFBR2 genes were selected and verified to be the downstream genes of LOXL2. In conclusion, LOXL2 contributes to the genesis and progression of HCC cells and works by regulating downstream genes of LOXL2 in certain pathways. Therefore, LOXL2 may play an important role in the progression and prognosis of HCC.

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Current Approach to Hepatocellular Carcinoma

Cited by 52 publications

References 64 publications

Hypothermic ante situm Resection in Tumors of the Hepatocaval Confluence

Hypothermic ante situm Resection in Tumors of the Hepatocaval Confluence

Mechanism of apoptosis induction in human hepatocellular carcinoma cells following treatment with a gecko peptides mixture

Mechanism Analyses for Elucidating the Role of LOXL2 Silencing in Hepatocellular Carcinoma

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