Current Applications of PRP and SVF Fractions in Stem Cell Therapy

Abstract

The ease of harvesting stromal vascular fraction (SVF) and the preponderance of progenitor cell populations with proven in vitro expansion and multilineage differentiation potential make it a sort after for regenerative therapy. Also, a complex of growth factors and other cytokines recoverable from platelet rich plasma (PRP) are crucial to a complete regenerative protocol in the treatment of organ and tissue defects. Nevertheless, we do know as tissues are created disparately and so are the bio-molecular requirements of damaged multifarious tissues. This article brings to bare recent facts on SVF and PRP physiological role in tissue regeneration modalities and as a guide for future exploit in the translational medicine. An internet database research of published articles from 2015 till date was conducted with average age of study group being below 50years but not morbidly obese as it concerns advances in applications of platelet rich plasma and stromal vascular fraction. GID-SVF1 separation kit offers the fastest SVF Isolation time of ~90 minutes compared to other techniques with no significant difference in SVF yields and preserved viability of isolated TNC highest at (81.47% ± 1.44) especially in young adults. This systematic best evidence review shows that autologous growth factor in PRP stimulate neoangiogenesis when injected fortnightly to initiate wound closure and re-epithelialization in chronic ulcers and burns especially where previous treatments had failed. Concomitant use of PRP and SVF was also found to improve skin regeneration, facial rejuvenation, facial scars, soft tissue defects and breast reconstruction surgeries. Anatomic origin and age of fat cells is crucial to their adipogenic, differentiation and commitment abilities with male superficial fat differentiating faster. However, an assessment of 'peroxisome proliferator-activated receptor-γ' (PPAR-γ) and PPAR-ɣ-2 indicator will be a guide.