Current applications of miniaturized chromatographic and electrophoretic techniques in drug analysis

Aturki, Zeineb; Rocco, Anna; Rocchi, Silvia; Fanali, Salvatore

doi:10.1016/j.jpba.2014.03.041

Cited by 57 publications

(41 citation statements)

References 118 publications

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“…The EOF can be controlled by selecting the experimental conditions (buffer, pH, ionic strength, applied voltage, organic solvent, etc.). The capillary column contains the SP (generally silica‐based particles, monolithic material, or wall‐bonded groups) …”

Section: Miniaturization In Electromigration and Liquid Chromatograpmentioning

confidence: 99%

Chiral Separations using Miniaturized Techniques: State of the Art and Perspectives

Fanali

2016

Israel Journal of Chemistry

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The separation and analysis of chiral compounds is an important topic of research, because these analytes are involved in various processes, e.g., biological, pharmacological, human health, etc. Their separation has also been recently obtained utilizing microfluidic techniques, such as capillary electrophoresis (CE), electrochromatography (CEC), and capillary/nano‐liquid chromatography (CLC/nano‐LC). Enantiomeric separation is mainly obtained by applying the direct resolution method, where a chiral selector (CS) is present in the separation system, either added to the mobile phase (MP) or bonded to the stationary phase or to the capillary wall. Among the several CSs used in microfluidic techniques, cyclodextrins, proteins, peptides, glycopeptide antibiotics, and modified polysaccharides are the most employed. Microfluidic techniques can offer several advantages over conventional analytical tools, e.g., high efficiency, high mass sensitivity, and reduced volumes of MP and sample. Optimized methods have been applied in different fields, such as biomedical, food chemistry, environment, pharmaceutical, etc. Excellent results have been achieved in CE and nano‐LC. Although CEC can offer higher efficiency due to the use of a strong electroosmotic flow, its use is less popular than the other. This limitation can be explained with the limited number of the stationary phases (SPs) commercially available.

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Section: Miniaturization In Electromigration and Liquid Chromatograpmentioning

confidence: 99%

Chiral Separations using Miniaturized Techniques: State of the Art and Perspectives

Fanali

2016

Israel Journal of Chemistry

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“…Enantiyomerlerin fiziksel ve kimyasal özelliklerinin aynı olması nedeniyle kiral bileşiğin (+) ve (-) enantiyomerlerine ayrılması oldukça zordur [13]. [34]. Kiral farmasötiklerin enantiyomerik ayrımında genellikle optik izomerlerin ayrımı doğrudan ve dolaylı olmak üzere iki şekilde gerçekleştirilmektedir.…”

Section: Kiral Kirleticilerin Analiziunclassified

Chiral pollutants and their significance in the environment

Can‐Güven¹,

Bolat²,

Gedik³

et al. 2016

Pamukkale J Eng Sci

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“…However, nowadays, HPLC is the technique more often used . The high demand for analytical tools, reliable and fast methodologies has recently stirred the interest of pharmaceutical industry toward miniaturized separation techniques, which furthermore provide reduced waste production and are cost effective . In this sense, CE is a miniaturized technique that offers some advantages with respect to chromatography techniques such as its high efficiency, resolution, and reduced volume reagents and organic solvents, with the consequent decrease of waste generation, environmental pollution, and cost .…”

Section: Introductionmentioning

confidence: 99%

On‐line preconcentration strategy for the simultaneous quantification of three local anesthetics in human urine using CZE

et al. 2015

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The simultaneous determination of usually employed anesthetics (procaine, lidocaine, and bupivacaine) has been developed and validated using CE with ultraviolet detection at 212 nm. The separation of these three drugs has been achieved in less than 7 min, using a temperature of 25ºC and 25 kV, with a 150 mM citrate buffer (pH 2.5) as BGE. Field-amplified sample injection (FASI) has been used for on-line sample preconcentration. Ultrapure water and ACN 50/50 (v/v) mixture gave the greatest enhancement factor when it was employed as an injection solvent. Injection voltage and time were optimized, being 13 kV and 13 s, the optimum values, respectively. To avoid the possible irreproducibility associated with the electrokinetic injection, an internal standard such as tetracaine, was employed. The instrumental detection limits (LOD S/N = 3) for the compounds ranged between 2.6 and 7.0 μg L(-1) and the quantitation limits (LOQ S/N = 10) between 37.8 and 55.9 μg L(-1) . The detection limits obtained in real human urine samples ranged between 55.2 and 83.6 μg L(-1) and the quantitation limits between 196.0 and 276.0 μg L(-1) . The proposed method has demonstrated its applicability to the analysis of these local anesthetics in urine samples without any pretreatment, allowing the rapid determination of these target analytes.

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Current applications of miniaturized chromatographic and electrophoretic techniques in drug analysis

Cited by 57 publications

References 118 publications

Chiral Separations using Miniaturized Techniques: State of the Art and Perspectives

Chiral Separations using Miniaturized Techniques: State of the Art and Perspectives

Chiral pollutants and their significance in the environment

On‐line preconcentration strategy for the simultaneous quantification of three local anesthetics in human urine using CZE

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