Current and next-generation bluetongue vaccines: Requirements, strategies, and prospects for different field situations

Feenstra, Femke; Rijn, Piet A. van

doi:10.1080/1040841x.2016.1186005

Cited by 41 publications

(42 citation statements)

References 113 publications

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“…Nowadays, the availability of a viral isolate for vaccine development is not as crucial as it was in the past. As for the existence of reverse genetics techniques (Boyce, Celma, & Roy, ) and synthetic gene production, the immediate availability of the Seg‐2 sequence may result in the fast production of engineered vaccines that can be potentially used rapidly in the field (Feenstra, Pap, & van Rijn, ; Feenstra & van Rijn, ; Nunes et al., ). Importantly, active circulation of BTV‐3 is further supported by the serological evidence of specific BTV‐3 antibodies in cattle and sheep collected in several regions of Libya during 2015 (Mahmoud et al., manuscript in preparation).…”

Section: Resultsmentioning

confidence: 99%

A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016

Sghaier

Lorusso

Portanti

et al. 2017

Transbound Emerg Dis

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Since 1998, southern Europe has experienced multiple incursions of different serotypes and topotypes of Bluetongue virus, a vector-borne transmitted virus, the causative agent of Bluetongue (BT), a major disease of ruminants. Some of these incursions originated from northern Africa, likely because of wind-blown dissemination of infected midges. In this report, we describe the detection and whole genome characterization of a novel BTV-3 strain identified in a symptomatic sheep in Tunisia. Sequences were immediately deposited with the GenBank Database under Accession Nos KY432369-KY432378. Alert and preparedness are requested to face the next vector seasons in northern Africa and the potential incursion of this novel strain in southern Europe.

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Section: Resultsmentioning

confidence: 99%

A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016

Sghaier

Lorusso

Portanti

et al. 2017

Transbound Emerg Dis

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“…It is likely that it would also be blocked in virus release and dissemination in blood-fed midges. Taken together, a small deletion in NS3/NS3a protein encompassing Late Domain is sufficient to establish nonvirulence, the DIVA principle (Differentiating Infected from Vaccinated), and the DISA principle (Disabled Infectious Single Animal) (reviewed in [38]).…”

Section: Discussionmentioning

confidence: 99%

Vector competence is strongly affected by a small deletion or point mutations in bluetongue virus

et al. 2019

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Background: Transmission of vector-borne virus by insects is a complex mechanism consisting of many different processes; viremia in the host, uptake, infection and dissemination in the vector, and delivery of virus during bloodfeeding leading to infection of the susceptible host. Bluetongue virus (BTV) is the prototype vector-borne orbivirus (family Reoviridae). BTV serotypes 1-24 (typical BTVs) are transmitted by competent biting Culicoides midges and replicate in mammalian (BSR) and midge (KC) cells. Previously, we showed that genome segment 10 (S10) encoding NS3/NS3a protein is required for virus propagation in midges. BTV serotypes 25-27 (atypical BTVs) do not replicate in KC cells. Several distinct BTV26 genome segments cause this so-called 'differential virus replication' in vitro. Methods: Virus strains were generated using reverse genetics and their growth was examined in vitro. The midge feeding model has been developed to study infection, replication and disseminations of virus in vivo. A laboratory colony of C. sonorensis, a known competent BTV vector, was fed or injected with BTV variants and propagation in the midge was examined using PCR testing. Crossing of the midgut infection barrier was examined by separate testing of midge heads and bodies. Results: A 100 nl blood meal containing ±10 5.3 TCID 50 /ml of BTV11 which corresponds to ±20 TCID 50 infected 50% of fully engorged midges, and is named one Midge Alimentary Infective Dose (MAID 50). BTV11 with a small in-frame deletion in S10 infected blood-fed midge midguts but virus release from the midgut into the haemolymph was blocked. BTV11 with S1[VP1] of BTV26 could be adapted to virus growth in KC cells, and contained mutations subdivided into 'corrections' of the chimeric genome constellation and mutations associated with adaptation to KC cells. In particular one amino acid mutation in outer shell protein VP2 overcomes differential virus replication in vitro and in vivo. Conclusion: Small changes in NS3/NS3a or in the outer shell protein VP2 strongly affect virus propagation in midges and thus vector competence. Therefore, spread of disease by competent Culicoides midges can strongly differ for very closely related viruses.

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“… 87 It must also be noted that current vaccines which target VP2 are serotype specific and give short-lived protection. 88 …”

Section: Discussionmentioning

confidence: 99%

Analysis of Conserved, Computationally Predicted Epitope Regions for VP5 and VP7 Across three Orbiviruses

Russell

Parbhoo

Gildenhuys

2018

Bioinform Biol Insights

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Orbiviruses are double-stranded RNA viruses that have profound economic and veterinary significance, 3 of the most important being African horse sickness virus (AHSV), bluetongue virus (BTV), and epizootic hemorrhagic disease virus (EHDV). Currently, vaccination and vector control are used as preventative measures; however, there are several problems with the current vaccines. Comparing viral amino acid sequences, we obtained an AHSV-BTV-EHDV consensus sequence for VP5 (viral protein 5) and for VP7 (viral protein 7) and generated homology models for these proteins. The structures and sequences were analyzed for amino acid sequence conservation, entropy, surface accessibility, and epitope propensity, to computationally determine whether consensus sequences still possess potential epitope regions. In total, 5 potential linear epitope regions on VP5 and 11 on VP7, as well as potential discontinuous B-cell epitopes, were identified and mapped onto the homology models created. Regions identified for VP5 and VP7 could be important in vaccine design against orbiviruses.

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Current and next-generation bluetongue vaccines: Requirements, strategies, and prospects for different field situations

Cited by 41 publications

References 113 publications

A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016

A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016

Vector competence is strongly affected by a small deletion or point mutations in bluetongue virus

Analysis of Conserved, Computationally Predicted Epitope Regions for VP5 and VP7 Across three Orbiviruses

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