Current and future techniques for detecting oxytocin: Focusing on genetically-encoded GPCR sensors

Lee, Dongmin; Kwon, Hyung-Bae

doi:10.1016/j.jneumeth.2021.109407

Cited by 7 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other GPCR-based sensors include the hormone oxytocin sensors 335 (includes GFP, FRET, BRET-based) and the neuropeptide orexin sensor 336 (OxLight, cpGFP-based). Whilst the indirect detection of targets, for example, the G q/11 -coupled receptors 337 (which increases intracellular Ca 2+ concentration) occurs via the lighting-up of GCaMP by Ca 2+ , the design and screening of GPCR-based sensors are not easy work.…”

Section: Genetically Encoded Fluorescent Probesmentioning

confidence: 99%

“…Its most recently improved version, GACh 3.0 (ACh 3.0), has the maximum fluorescence response DF/F 0 of B280%, and can be used to detect the cholinergic activity in mice and drosophila with almost no interference from nicotine, choline, glycine or other neurotransmitters. 334 Other GPCR-based sensors include the hormone oxytocin sensors 335 (includes GFP, FRET, BRET-based) and the neuropeptide orexin sensor 336 (OxLight, cpGFP-based). Whilst the indirect detection of targets, for example, the G q/11 -coupled receptors 337 (which increases intracellular Ca 2+ concentration) occurs via the lighting-up of GCaMP by Ca 2+ , the design and screening of GPCR-based sensors are not easy work.…”

Section: Neurotransmitter Sensorsmentioning

confidence: 99%

See 1 more Smart Citation

Fluorescent proteins and genetically encoded biosensors

Wang

Tian

2023

Chem. Soc. Rev.

View full text Add to dashboard Cite

show abstract

Section: Genetically Encoded Fluorescent Probesmentioning

confidence: 99%

Section: Neurotransmitter Sensorsmentioning

confidence: 99%

Fluorescent proteins and genetically encoded biosensors

Wang

Tian

2023

Chem. Soc. Rev.

View full text Add to dashboard Cite

show abstract

“…We demonstrated the PnP capability of LAUNCHER in the design of custom biological circuits using our previous iTango2 system, which is a genetically encoded GPCR sensor that responds to both blue light and GPCR ligand 16,39,40 . DRD2-iTango2 consists of two separate polypeptides, a plasma membranetethered DRD2-TevN-iLID-TEVseq-tTA and a cytosolic βarrestin2 (βArr2)-TevC.…”

Section: Pnp Of Launcher To Enhance the Performance Of Protease-based...mentioning

confidence: 99%

LAUNCHER: A single-component, light-assisted uncaging switch for endoproteolytic release

Cui¹,

Lee²,

Ban³

et al. 2022

Preprint

View full text Add to dashboard Cite

Proteases can serve as molecular switches to initiate a wide spectrum of biological events. High efficiency and specificity enhance the potential of proteases as versatile elements in synthetic biological circuits. To address the unmet need for a novel protease-based toolkit with modular features, we developed a single-component photocleavable switch, termed LAUNCHER (Light-Assisted UNcaging switCH for Endoproteolytic Release), combining a circularly permuted potyviral protease and a blue-light-gated substrate with variable intermodular linkers. LAUNCHER enables user-controllable release of payloads that can serve in various cellular applications, such as transgene expression, multilayer-based G protein-coupled receptor (GPCR) activity sensing, and optochemogenetic manipulation. Moreover, LAUNCHER orthologs from the orthogonal potyviral protease family can be utilized in a multiple protease-based circuit without mutual interference. LAUNCHER provides a generalized method to implant optogenetic manipulations into existing synthetic biological circuits in a plug-and-play manner and adds to optogenetic toolboxes for the expansion of biomedical fields.

show abstract

“…This optogenetic platform enables labeling of oxytocin sensitive neurons for post-experimentation analysis of oxytocinergic cell activation but lacks the temporal resolution requisite for real-time oxytocin imaging. Selectivity, particularly against vasopressin, and slow temporal resolution limitations continue to pose a challenge for OXTR- and expression-dependent sensors, respectively 21 .…”

Section: Introductionmentioning

confidence: 99%

Near Infrared Nanosensors Enable Optical Imaging of Oxytocin with Selectivity over Vasopressin in Acute Mouse Brain Slices

Navarro

Jeong

Ouassil

et al. 2022

Preprint

View full text Add to dashboard Cite

Oxytocin plays a critical role in regulating social behaviors, yet our understanding of its role in both neurological health and disease remains incomplete. Real-time oxytocin imaging probes with the spatiotemporal resolution relevant to its endogenous signaling are required to fully elucidate oxytocin function in the brain. Herein we describe a near-infrared oxytocin nanosensor (nIROx), a synthetic probe capable of imaging oxytocin in the brain without interference from its structural analogue, vasopressin. nIROx leverages the inherent tissue-transparent fluorescence of single-walled carbon nanotubes (SWCNT) and the molecular recognition capacity of an oxytocin receptor peptide fragment (OXTp) to selectively and reversibly image oxytocin. We employ these nanosensors to monitor electrically stimulated oxytocin release in brain tissue, revealing oxytocin release sites with a median size of 3 μm which putatively represents the spatial diffusion of oxytocin from its point of release. These data demonstrate that covalent SWCNT constructs such as nIROx are powerful optical tools that can be leveraged to measure neuropeptide release in brain tissue.

show abstract

Current and future techniques for detecting oxytocin: Focusing on genetically-encoded GPCR sensors

Cited by 7 publications

References 51 publications

Fluorescent proteins and genetically encoded biosensors

Fluorescent proteins and genetically encoded biosensors

LAUNCHER: A single-component, light-assisted uncaging switch for endoproteolytic release

Near Infrared Nanosensors Enable Optical Imaging of Oxytocin with Selectivity over Vasopressin in Acute Mouse Brain Slices

Contact Info

Product

Resources

About