Current and Future Role of Natural-Killer Cells in Cancer Immunotherapy

Harlan, Lisa M.; Djeu, Julie Y.

doi:10.1385/1-59745-011-1:045

Cited by 1 publication

(1 citation statement)

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“…In a tumor microenvironment, numerous cells of innate and adaptive immunity produce IFN-γ. Two main producers of IFN-γ are natural killer (NK) cells, providing signals from their activating receptors that prevail over signals stemming from inhibitory receptors, and tumor-specific cytotoxic CD8+ T lymphocytes (CTLs) following antigen stimulation of T cell receptor (TCR) or in TCR-independent way that involves synergistic stimulation with cytokines IL-12 and IL-18 [19]. Th1 polarized CD4+ T helper cells, known for their ability to help in the promotion and maintenance of anti-tumor CTL responses, also secrete IFN-γ that recruits various cells of innate and adaptive immunity to tumor sites and promote their activation.…”

Section: Discussionmentioning

confidence: 99%

Proliferative Effects of Lung Cancer Cells Derived Exosomes on T Cells

Hussain

Malik

2022

Braz. arch. biol. technol.

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Lung cancer patients have higher expression of Exosomes (EXs) carrying several bioactive molecules including lipids, protein, carbohydrates, and different types of RNAs. Upon delivery of EXs into the recipient cells different behavioral changes are observed in the biological mechanisms of the cell. In the current study, we aimed to compare the EXs production in lung cancer cells (H1975) in two different conditions including normal conditions (NC) (20% O2, 5% CO2, 10% FBS and 100 U/mL penicillin and 100 μg/mL streptomycin added media with pH 7) and our established conditions (EC) (1% O2, 5% CO2 and FBS/antibiotic-free media with pH 6). We also studied the proliferative effects of these EXs (NC and EC) on Jurkat cells (immortalized T lymphocytes) and proliferation of some of the human T cells population was also observed with co-culture experiments. From the Nano sight analysis, a high quantity of EXs was observed in EC as compared with the NC. EXs isolated from both conditions showed proliferative effects on Jurkat cells. Among the human T cells population, EC-EXs were shown low expression of CD8-IFNγ and CD4-IFN γ in comparison with NC-EXs. These results support the hypothesis that Lung cancer cells derived EXs can interact with the T cells mechanisms leading to the low immune response in the cancer microenvironment and providing a favorable condition for cancer cells. Such studies need validation in vivo to verify the actual mechanisms which could be effective in potential therapeutic strategies.

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Section: Discussionmentioning

confidence: 99%