“…Inactivated vaccines were efficient in decreasing S. Enteritidis in broiler breeders [ 36 ], and when following the combined application of both live and killed vaccines, the protection against infection exceeded the performance of either product administered separately [ 37 , 38 , 39 ]. Most of the commercial inactivated vaccines are composed of killed cells of S. Enteritidis and S. Typhimurium [ 31 ]; others include S. Infantis [ 39 ], S. Heidelberg, or even local or regional strains of a certain serovar of importance [ 40 ].…”
Worldwide, poultry infections by Salmonella are the cause of significant economic losses, not only due to reduced production (due to fowl typhoid disease), but also considering the efforts and control measures that must be constantly applied, especially due to zoonotic serovars. Poultry is a common reservoir of Salmonella and its transmission into the food chain is a risk for humans. The vaccination of layers plays an important role in the overall efforts to prevent Salmonella infections. An inactivated trivalent vaccine was prepared with S. Enteritidis, S. Typhimurium, and S. Infantis strains. Infection trials were performed to evaluate the efficacy of three vaccination schedules using inactivated and live S. Gallinarum 9R vaccines. For this purpose, at week 5 of life, one subcutaneous dose of live S. Gallinarum 9R vaccine (1–5 × 107 CFU) was given to Groups 1 and 2. At weeks 8 and 11 of life, chickens were also vaccinated with one (Group 1) or two (Groups 2 and 3) intramuscular doses of the inactivated oil-adjuvant trivalent vaccine (1 × 108 CFU/dose of each antigen). Group 4 consisted of chickens that remained unvaccinated (control). At week 14 of life, the efficacy of the vaccination plans was evaluated in three separate inoculation trials with S. Enteritidis, S. Typhimurium, or S. Infantis. After vaccination with the inactivated vaccine, homologous antibody production was observed, and after challenge, a significant reduction in the faecal shedding, invasion, and colonization of S. Typhimurium and S. Infantis was achieved by all vaccination schedules, while the vaccination with at least one dose of the live S. Gallinarum 9R vaccine was necessary to obtain such a significant protection against S. Enteritidis infection.
“…Inactivated vaccines were efficient in decreasing S. Enteritidis in broiler breeders [ 36 ], and when following the combined application of both live and killed vaccines, the protection against infection exceeded the performance of either product administered separately [ 37 , 38 , 39 ]. Most of the commercial inactivated vaccines are composed of killed cells of S. Enteritidis and S. Typhimurium [ 31 ]; others include S. Infantis [ 39 ], S. Heidelberg, or even local or regional strains of a certain serovar of importance [ 40 ].…”
Worldwide, poultry infections by Salmonella are the cause of significant economic losses, not only due to reduced production (due to fowl typhoid disease), but also considering the efforts and control measures that must be constantly applied, especially due to zoonotic serovars. Poultry is a common reservoir of Salmonella and its transmission into the food chain is a risk for humans. The vaccination of layers plays an important role in the overall efforts to prevent Salmonella infections. An inactivated trivalent vaccine was prepared with S. Enteritidis, S. Typhimurium, and S. Infantis strains. Infection trials were performed to evaluate the efficacy of three vaccination schedules using inactivated and live S. Gallinarum 9R vaccines. For this purpose, at week 5 of life, one subcutaneous dose of live S. Gallinarum 9R vaccine (1–5 × 107 CFU) was given to Groups 1 and 2. At weeks 8 and 11 of life, chickens were also vaccinated with one (Group 1) or two (Groups 2 and 3) intramuscular doses of the inactivated oil-adjuvant trivalent vaccine (1 × 108 CFU/dose of each antigen). Group 4 consisted of chickens that remained unvaccinated (control). At week 14 of life, the efficacy of the vaccination plans was evaluated in three separate inoculation trials with S. Enteritidis, S. Typhimurium, or S. Infantis. After vaccination with the inactivated vaccine, homologous antibody production was observed, and after challenge, a significant reduction in the faecal shedding, invasion, and colonization of S. Typhimurium and S. Infantis was achieved by all vaccination schedules, while the vaccination with at least one dose of the live S. Gallinarum 9R vaccine was necessary to obtain such a significant protection against S. Enteritidis infection.
“…Typhimurium strain UK-1 (ATCC 68169) is the wild-type parent strain used as the foundation for the majority of the attenuated recombinant and non-recombinant vaccine derivatives studied in our laboratory [ 21 ]. UK-1 strain χ3761 was the parent strain from which many commercial vaccines for poultry such as Megan®Egg and Megan®Vac were also derived [ 21 – 25 ]. Since UK-1 is highly pathogenic in several hosts including mice and chickens [ 21 , 26 – 28 ], these vaccine derivatives, when orally administered, are presumed to be more immunogenic and hence trigger a greater level of protective immunity than vaccine derivatives from less-virulent S .…”
The initial virulence and invasiveness of a bacterial strain may play an important role in leading to a maximally efficacious attenuated live vaccine. Here we show that χ9909, derived from Salmonella Typhimurium UK-1 χ3761 (the most virulent S. Typhimurium strain known to us), is effective in protecting mice against lethal UK-1 and 14028S (less virulent S. Typhimurium strain) challenge. As opposed to this, 14028S-derived vaccine χ12359 induces suboptimal levels of protection, with survival percentages that are significantly lower when challenged with lethal UK-1 challenge doses. T-cell assays have revealed that significantly greater levels of Th1 cytokines IFN-γ and TNF-α were secreted by stimulated T-lymphocytes obtained from UK-1(ΔaroA) immunized mice than those from mice immunized with 14028S(ΔaroA). In addition, UK-1(ΔaroA) showed markedly higher colonizing ability in the spleen, liver, and cecum when compared to 14028S(ΔaroA). Enumeration of bacteria in fecal pellets has also revealed that UK-1(ΔaroA) can persist in the host for over 10 days whereas 14028S(ΔaroA) titers dropped significantly by day 10. Moreover, co-infection of parent strains UK-1 and 14028S resulted in considerably greater recovery of the former in multiple mucosal and gut associated lymphatic tissues. Mice immunized with UK-1(ΔaroA) were also able to clear UK-1 infection remarkably more efficiently from the target organs than 14028S(ΔaroA). Together, these results provide ample evidence to support the hypothesis that attenuated derivatives of parent strains with higher initial virulence make better vaccines.
“…The Vector Control group was dropped because of the limited facility capacity and its disadvantage as observed in Trials 1 and 2. Broiler vaccines are generally delivered via coarse spray in the hatchery or via drinking water in broiler houses ( Aehle and Curtiss, 2017 ) in addition to in ovo immunization. A virulent C. perfringens strain CP4, which leads to 40% mortality in nonvaccinated (NV) broilers challenged with this strain, was used as the challenge strain to evaluate the broad effectiveness of the vaccine ( Table 4 ).…”
Gram-positive
Clostridium perfringens
type G, the causative agent of necrotic enteritis (
NE
), has gained more attention in the poultry industry due to governmental restrictions on the use of growth-promoting antibiotics in poultry feed. Our previous work has proved that regulated delayed lysis
Salmonella
vaccines delivering a plasmid encoding an operon fusion of the nontoxic C-terminal adhesive part of alpha toxin and a GST-NetB toxin fusion were able to elicit significant protective immunity in broilers against
C. perfringens
challenge. We recently improved our
S.
Typhimurium antigen delivery vaccine strain by integrating a rhamnose-regulated O-antigen synthesis gene enabling a triple-sugar regulation system to control virulence, antigen-synthesis and lysis in vivo traits. The strain also includes a Δ
sifA
mutation that was previously shown to increase the immunogenicity of and level of protective immunity induced by
Salmonella
vectored influenza and
Eimeria
antigens. The new antigen-delivery vaccine vector system confers on the vaccine strain a safe profile and improved protection against
C. perfringens
challenge. The strain with the triple-sugar regulation system delivering a regulated lysis plasmid pG8R220 encoding the PlcC and GST-NetB antigens protected chickens at a similar level observed in antibiotic-treated chickens. Feed conversion and growth performance were also similar to antibiotic-treated chickens. These studies made use of a severe
C. perfringens
challenge with lesion formation and mortality enhanced by pre-exposure to
Eimeria maxima
oocysts. The vaccine achieved effectiveness through three different immunization routes, oral, spray and in drinking water. The vaccine has a potential for application in commercial hatcher and broiler-rearing conditions.
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