Current and future perspectives for dentin-pulp tissue engineering - an update

Srivastava, Swati

doi:10.17159/2519-0105/2019/v74no3a1

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…This is due to the fact that regenerated tissue does not completely match the normal healthy functional tissue. Hence, a novel dental bioengineering approach has been suggested consisting of the three classic elements (i.e., dental stem cells, signal molecules, and scaffolds) and two new ones (i.e., stem cell niches and survival factors) [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…Bioactive molecules are signaling factors or chemical cues that regulate a variety of cellular responses by specific binding to the transmembrane or the intracellular receptors on a target cell. The bone morphogenetic protein (BMP) superfamily of proteins such as BMP-2, BMP-4, BMP-7, and transforming growth factor beta-1 (TGF-β1) has been found to modulate cellular functions [2]. TGF-β is a signaling molecule that induces cell proliferation, cell differentiation, chemotaxis, and apoptosis in monocytes and epithelial, mesenchymal, and neuronal cells.…”

Section: Introductionmentioning

confidence: 99%

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TRANSFORMING GROWTH FACTOR-β1 EXPRESSION IN VARIOUS CONCENTRATIONS OF ADVANCED PLATELET-RICH FIBRIN MODULATING HUMAN DENTAL PULP STEM CELL DIFFERENTIATION

et al. 2020

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Objective: Modification of the speed and time of centrifugation based on the low-speed centrifugation concept for platelet-rich fibrin (PRF) has resulted in a new type of PRF known as advanced PRF (A-PRF). A-PRF can release several types of growth factors (GFs) that participate in the process of differentiation, such as transforming GF-β1 (TGF-β1). The aim of this study was to analyze TGF-β1 expression in various concentrations of A-PRF in the differentiation process of human dental pulp stem cells (hDPSCs).Methods: hDPSC cultures were obtained from those of previous research (ethical approval form has been attached). These hDPSCs were in the 2nd–3rd passage, and serum starvation was done by reducing fetal bovine serum (FBS) levels in the hDPSC culture media. A-PRF was obtained using 10 ml blood collected from the cubital vein, which was centrifuged at 1500 rpm for 14 min and then divided into four concentration groups. TGF-β1 expression in 1%, 5%, and 25% A-PRF as well as in 10% FBS (control) was analyzed by ELISA on day 7.Results: Although no significant differences were observed in TGF-β1 expression between 1%, 5%, and 25% A-PRF, and 10% FBS, it was observed that the higher the concentration of A-PRF, the greater the TGF-β1 expression.Conclusion: The expression of TGF-β1 was consistent with the increase in A-PRF concentration. The highest TGF-β1 expression was detected in 25% A-PRF among all concentrations in the differentiation process of hDPSCs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

TRANSFORMING GROWTH FACTOR-β1 EXPRESSION IN VARIOUS CONCENTRATIONS OF ADVANCED PLATELET-RICH FIBRIN MODULATING HUMAN DENTAL PULP STEM CELL DIFFERENTIATION

et al. 2020

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Application of neurotransmitters and dental stem cells for pulp regeneration: A review

Ramli

Yusop

Ramli

et al. 2023

The Saudi Dental Journal

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Increased VEGF-A Expression of Human Dental Pulp Stem Cells (hDPSCs) Cultured with Advanced Platelet Rich Fibrin (A-PRF)

Bagio¹,

Julianto²,

Margono³

et al. 2021

TODENTJ

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Background: VEGF-A expression of human dental pulp stem cells (hDPSCs) can induce the angiogenesis process of dental pulp regeneration. This in vitro study aimed to analyze the effect of various concentrations of Advanced Platelet Rich Fibrin (A-PRF) conditioned media (CM) on the increased expression of vascular endothelial growth factor-A (VEGF-A) of hDPSCs. Methods: hDPSCs were collected from ten third molars extracted from nine healthy donors, cultured, and then harvested at the end of the third passage. The hDPSCs were seeded in four different CM (control group: hDPSCs + DMEM; 1% A-PRF CM group: hDPSCs + 1% A-PRF CM; 5% A-PRF CM group: hDPSCs + 5% A-PRF CM; 10% A-PRF CM group: hDPSCs + 10% A-PRF CM). All of the groups were cultured in biological triplicates (Triplo) and observed for 5, 12, and 24 hours. The VEGF-A protein expression of hDPSCs was measured using human VEGF-A ELISA at a wavelength of 405 nm. Data was analyzed with Kruskal Wallis and post hoc Mann Whitney test with p<0.05. Results: The VEGF-A expression rate of hDPSCs among all groups was statistically significantly different at 5, 12 and 24 hours of observations (p<0.05). Post hoc analysis test showed a statistically significant difference of hDPSCs’s VEGF-A expression between 5% A-PRF groups compared to other groups at 5 and 12 hours of observation (p<0.05). However, there were no statistically significant differences observed of hDPSCs’ VEGF-A expression at 24 hours of observation between 1%, 5% and 10% A-PRF groups (p>0.05). Conclusion: 5% A-PRF CM was superior in increasing VEGF-A expression of hDPSCs at 5, 12 and 24 hours of observations.

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Current and future perspectives for dentin-pulp tissue engineering - an update

Abstract: 3. Synthetic polymers such as polylactic and polylactic-co-glycolic acids; 4. Biopolymers such as collagen, hyaluronan and chitosan; and self-assembling peptide hydrogel.

Cited by 5 publications

References 28 publications

TRANSFORMING GROWTH FACTOR-β1 EXPRESSION IN VARIOUS CONCENTRATIONS OF ADVANCED PLATELET-RICH FIBRIN MODULATING HUMAN DENTAL PULP STEM CELL DIFFERENTIATION

TRANSFORMING GROWTH FACTOR-β1 EXPRESSION IN VARIOUS CONCENTRATIONS OF ADVANCED PLATELET-RICH FIBRIN MODULATING HUMAN DENTAL PULP STEM CELL DIFFERENTIATION

Application of neurotransmitters and dental stem cells for pulp regeneration: A review

Increased VEGF-A Expression of Human Dental Pulp Stem Cells (hDPSCs) Cultured with Advanced Platelet Rich Fibrin (A-PRF)

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