Current and future molecular approaches to investigate the white pine blister rust pathosystem

Richardson, Bryce A.; Ekramoddoulah, A. K. M.; Liu, Jun‐Jun; Kim, M.-S.; Klopfenstein, Ned B.

doi:10.1111/j.1439-0329.2010.00660.x

Cited by 19 publications

(50 citation statements)

References 121 publications

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“…Whether there are alpine and lowland biotypes or native and invasive rusts would require additional study. R ichardson et al. (2010); and see their Fig.…”

Section: Historical Biogeographymentioning

confidence: 94%

“…R ichardson et al. (2009) presented preliminary results of a currently active but incomplete survey of the blister rusts of white pines (see R ichardson et al. 2010; : Fig.…”

Section: Taxonomy and Life Cyclementioning

confidence: 99%

“…2010). Revision of the systematics for the Cronartium rusts on white pines would require appropriate use of molecular diagnostic techniques (R ichardson et al. 2010).…”

Section: Introductionmentioning

confidence: 99%

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White pines, Ribes, and blister rust: a review and synthesis

2010

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For over a century, white pine blister rust (Cronartium ribicola) has linked white pines (Strobus) with currants and gooseberries (Ribes) in a complex and serious disease epidemic in Asia, Europe, and North America. Because of ongoing changes in climate, societal demands for forests and their amenities, and scientific advances in genetics and proteomics, our current understanding and management of the white pine blister rust pathosystem has become outdated. Here, we present a review and synthesis of international scope on the biology and management of blister rust, white pines, Ribes, and other hosts. In this article, we provide a geographical and historical background, describe the taxonomy and life cycle of the rust, discuss pathology and ecology, and introduce a series of invited papers. These review articles summarize the literature on white pines, Ribes, and blister rust with respect to their status, threats, and management through genetics and silviculture. Although the principal focus is on North America, the different epidemics in Europe and Asia are also described. In the final article, we discuss several of the key observations and conclusions from the preceding review articles and identify prudent actions for research and management of white pine blister rust.

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“…Whether there are alpine and lowland biotypes or native and invasive rusts would require additional study. R ichardson et al. (2010); and see their Fig.…”

Section: Historical Biogeographymentioning

confidence: 94%

“…R ichardson et al. (2009) presented preliminary results of a currently active but incomplete survey of the blister rusts of white pines (see R ichardson et al. 2010; : Fig.…”

Section: Taxonomy and Life Cyclementioning

confidence: 99%

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White pines, Ribes, and blister rust: a review and synthesis

2010

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“…At the molecular level, a few studies revealed several Pinus gene families involved in defense response against C. ribicola infection. Plant R family of NBS-LRR proteins and multiple families of PR proteins, including chitinases (PR3), thaumatin like proteins (PR5), intracellular ribonuclease-like proteins (PR10), and anti-microbial peptides/proteins (AMPs), contributed in part to host resistance in the WPBR interactions [41,100].…”

Section: Genomic Selection Of Partial Resistancementioning

confidence: 99%

Genetic Resistance to Fusiform Rust in Southern Pines and White Pine Blister Rust in White Pines—A Contrasting Tale of Two Rust Pathosystems—Current Status and Future Prospects

Sniezko

Smith

Liu

et al. 2014

Forests

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Damage or mortality from pathogens can reduce productivity of forest plantations, as well as significantly harm natural forest ecosystems. Genetic resistance within the host species is the first line of defense for tree species. Resistance breeding programs for the native fusiform rust and exotic (to North America) white pine blister rust diseases are two of the longest concerted efforts in forest trees, spanning more than 50 years. Advances in developing greater genetic resistance have been made in both pathosystems, but unique challenges and opportunities in each system translate to different approaches. Fusiform rust resistance programs have mainly emphasized complete resistance, while partial resistance plays a prominent role in white pine blister rust resistance programs. Advances in the development of molecular genetic tools now permit investigations in conifers and their associated rust pathogens. Good progress has been made in identifying resistant populations and understanding resistance in these pathosystems, and resistant stock is now being used extensively for reforestation and restoration. These programs represent great success stories brought to fruition by the OPEN ACCESSForests 2014, 5 2051 long-term efforts. However, continued support will be needed to enhance the level and fully realize the potential of durable genetic resistance in these invaluable North American conifer species.

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“…For example, the plant disease resistance (R) family of NBS-LRR proteins and several families of pathogenesis-related (PR) proteins, including chitinases (PR3), thaumatin like proteins -TLPs (PR5), intracellular ribonuclease-like proteins (PR10), and anti-microbial peptides/proteins (AMPs), have been shown to contribute to host resistance in WP-BR interactions [5,6]. A recent proteomic profiling uncovered over one hundred P. monticola proteins modulated by C. ribicola inoculation, which included heat shock proteins (HSPs), reactive oxygen species (ROS) scavenging enzymes, and intermediate factors functioning in the signal transduction pathways triggered by well-known plant R genes, as well as other defence-related proteins [7].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of Pinus monticola primary needles by RNA-seq provides novel insight into host resistance to Cronartium ribicola

2013

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BackgroundFive-needle pines are important forest species that have been devastated by white pine blister rust (WPBR, caused by Cronartium ribicola) across North America. Currently little transcriptomic and genomic data are available to understand molecular interactions in the WPBR pathosystem.ResultsWe report here RNA-seq analysis results using Illumina deep sequencing of primary needles of western white pine (Pinus monticola) infected with WPBR. De novo gene assembly was used to generate the first P. monticola consensus transcriptome, which contained 39,439 unique transcripts with an average length of 1,303 bp and a total length of 51.4 Mb. About 23,000 P. monticola unigenes produced orthologous hits in the Pinus gene index (PGI) database (BLASTn with E values < e-100) and 6,300 genes were expressed actively (at RPKM ≥ 10) in the healthy tissues. Comparison of transcriptomes from WPBR-susceptible and -resistant genotypes revealed a total of 979 differentially expressed genes (DEGs) with a significant fold change > 1.5 during P. monticola- C. ribicola interactions. Three hundred and ten DEGs were regulated similarly in both susceptible and resistant seedlings and 275 DEGs showed regulatory differences between susceptible and resistant seedlings post infection by C. ribicola. The DEGs up-regulated in resistant seedlings included a set of putative signal receptor genes encoding disease resistance protein homologs, calcineurin B-like (CBL)-interacting protein kinases (CIPK), F-box family proteins (FBP), and abscisic acid (ABA) receptor; transcriptional factor (TF) genes of multiple families; genes homologous to apoptosis-inducing factor (AIF), flowering locus T-like protein (FT), and subtilisin-like protease. DEGs up-regulated in resistant seedlings also included a wide diversity of down-stream genes (encoding enzymes involved in different metabolic pathways, pathogenesis-related -PR proteins of multiple families, and anti-microbial proteins). A large proportion of the down-regulated DEGs were related to photosystems, the metabolic pathways of carbon fixation and flavonoid biosynthesis.ConclusionsThe novel P. monticola transcriptome data provide a basis for future studies of genetic resistance in a non-model, coniferous species. Our global gene expression profiling presents a comprehensive view of transcriptomic regulation in the WPBR pathosystem and yields novel insights on molecular and biochemical mechanisms of disease resistance in conifers.

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Current and future molecular approaches to investigate the white pine blister rust pathosystem

Cited by 19 publications

References 121 publications

White pines, Ribes, and blister rust: a review and synthesis

White pines, Ribes, and blister rust: a review and synthesis

Genetic Resistance to Fusiform Rust in Southern Pines and White Pine Blister Rust in White Pines—A Contrasting Tale of Two Rust Pathosystems—Current Status and Future Prospects

Transcriptome analysis of Pinus monticola primary needles by RNA-seq provides novel insight into host resistance to Cronartium ribicola

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