Current and Emerging Applications of Metabolomics in the Field of Agricultural Biotechnology

Hill, Camilla Beate; Dias, Daniel A.; Roessner, Ute

doi:10.1002/9781118864463.ch02

Cited by 3 publications

(2 citation statements)

References 79 publications

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“…Untargeted GC–MS analysis and data analysis were carried out as described in Hill, Dias, & Roessner, . The metabolite data were analyzed with the open‐source software, MetaboAnalyst 3.0 (Xia & Wishart, ).…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and metabolic plasticity shapes life‐history strategies under combinations of abiotic stresses

et al. 2019

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Plants developed various reversible and non‐reversible acclimation mechanisms to cope with the multifaceted nature of abiotic‐stress combinations. We hypothesized that in order to endure these stress combinations, plants elicit distinctive acclimation strategies through specific trade‐offs between reproduction and defense. To investigate Brachypodium distachyon acclimation strategies to combinations of salinity, drought and heat, we applied a system biology approach, integrating physiological, metabolic, and transcriptional analyses. We analyzed the trade‐offs among functional and performance traits, and their effects on plant fitness. A combination of drought and heat resulted in escape strategy, while under a combination of salinity and heat, plants exhibited an avoidance strategy. On the other hand, under combinations of salinity and drought, with or without heat stress, plant fitness (i.e., germination rate of subsequent generation) was severely impaired. These results indicate that under combined stresses, plants’ life‐history strategies were shaped by the limits of phenotypic and metabolic plasticity and the trade‐offs between traits, thereby giving raise to distinct acclimations. Our findings provide a mechanistic understanding of plant acclimations to combinations of abiotic stresses and shed light on the different life‐history strategies that can contribute to grass fitness and possibly to their dispersion under changing environments.

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Section: Methodsmentioning

confidence: 99%

Phenotypic and metabolic plasticity shapes life‐history strategies under combinations of abiotic stresses

et al. 2019

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“…With the development of modern instruments more and more techniques have been used in the metabolomics study including chromatography, mass spectrometry, nuclear magnetic resonance (NMR) (Cevallos-Cevallos, Reyes-De-Corcuera, Etxeberria, Danyluk, & Rodrick, 2009). Among these methods, NMR-based metabolomics has proved a promising tool for food compositional analysis and food material characterization due to its capability to determine a great number of metabolites rapidly and simultaneously (Hill, Dias, & Roessner, 2016;Soininen et al, 2014). Currently, many multivariate data analyses approaches used in combination with 1 H-NMR metabolomics have been indicated as versatile analytical tools that can provide comprehensive information on the metabolic profiles in both vegetal and animal matrices, such as principal component analysis (PCA) (Francini et al, 2017), partial least-squares discriminant analysis (PLS-DA) (Pereira et al, 2005), Linear Discriminant Analysis (LDA) (Huo et al, 2017), k-Nearest Neighbor (KNN) (Xu, Song, Li, & Wan, 2012), Support vector machine (SVM) (Masoum et al, 2007), Least square support vector machine (LS-SVM) (Jin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Fast nutritional characterization of different pigmented rice grains using a combination of NMR and decision tree analysis

Huo

Liu

et al. 2019

CyTA - Journal of Food

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2019) Fast nutritional characterization of different pigmented rice grains using a combination of NMR and decision tree analysis, ABSTRACT Different rice cultivars contain various metabolites which are closely related with their nutritional and functional effects. In the current study, NMR and chemometrics methodsdecision tree analysis (DTA) were applied to explore the chemical characteristics in different rice cultivars. The pigmented rice were completely discriminated from each other, and the key metabolites that mostly contributed to the rice discrimination were explored, including sugars compounds (sucrose, fructose and glucose) and non-sugar compounds (γ-amino butyric acid (GABA), asparagine, citric acid and malic acid) with DTA approach and 1 HNMR metabolite fingerprints. The purple rice has the highest concentrations of sucrose, citric acid and asparagine (p < 0.05), but lowest fructose (p < 0.05); red rice has the lowest GABA concentration comparing with the other three groups (p < 0.05). This study implies that DTA method is a powerful tool to discriminate different pigmented rice varieties. Furthermore, it also reveals the crucial nutrients based on 1 H-NMR metabolic profiling. ARTICLE HISTORY

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Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species

Kiani‐Pouya

Roessner

Jayasinghe

et al. 2017

Plant Cell & Environment

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113

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Epidermal bladder cells (EBCs) have been postulated to assist halophytes in coping with saline environments. However, little direct supporting evidence is available. Here, Chenopodium quinoa plants were grown under saline conditions for 5 weeks. One day prior to salinity treatment, EBCs from all leaves and petioles were gently removed by using a soft cosmetic brush and physiological, ionic and metabolic changes in brushed and non-brushed leaves were compared. Gentle removal of EBC neither initiated wound metabolism nor affected the physiology and biochemistry of control-grown plants but did have a pronounced effect on salt-grown plants, resulting in a salt-sensitive phenotype. Of 91 detected metabolites, more than half were significantly affected by salinity. Removal of EBC dramatically modified these metabolic changes, with the biggest differences reported for gamma-aminobutyric acid (GABA), proline, sucrose and inositol, affecting ion transport across cellular membranes (as shown in electrophysiological experiments). This work provides the first direct evidence for a role of EBC in salt tolerance in halophytes and attributes this to (1) a key role of EBC as a salt dump for external sequestration of sodium; (2) improved K retention in leaf mesophyll and (3) EBC as a storage space for several metabolites known to modulate plant ionic relations.

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Current and Emerging Applications of Metabolomics in the Field of Agricultural Biotechnology

Cited by 3 publications

References 79 publications

Phenotypic and metabolic plasticity shapes life‐history strategies under combinations of abiotic stresses

Phenotypic and metabolic plasticity shapes life‐history strategies under combinations of abiotic stresses

Fast nutritional characterization of different pigmented rice grains using a combination of NMR and decision tree analysis

Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species

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