Current advances of succinate biosynthesis in metabolically engineered Escherichia coli

Zhu, Liwen; Tang, Ya‐Jie

doi:10.1016/j.biotechadv.2017.09.007

Cited by 27 publications

(23 citation statements)

References 121 publications

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“…In one case, a CRISPR/Cas9 protocol was optimized for scarless integration of large DNA fragments; 2.4, 3.9, 5.4, and 7 kb fragments were inserted with respective efficiencies of 91%, 92%, 71% and 61% (Chung et al, 2017). CRISPR/Cas9 has also been employed for optimizing expression of chromosomally contained metabolic pathways (Alonso-Gutierrez et al, 2017;Zhu et al, 2017;. For example, a CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique was developed to alter expression of chromosomally integrated genes (Zhu et al, 2017).…”

Section: Crisprmentioning

confidence: 99%

“…CRISPR/Cas9 has also been employed for optimizing expression of chromosomally contained metabolic pathways (Alonso-Gutierrez et al, 2017;Zhu et al, 2017;. For example, a CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique was developed to alter expression of chromosomally integrated genes (Zhu et al, 2017). Using CFPO, expression of multiple genes can be altered simultaneously.…”

Section: Crisprmentioning

confidence: 99%

“…Production of succinic acid by E. coli has a longer history than most of di-acids. These efforts are extensively reviewed elsewhere (Thakker et al, 2012;Cheng et al, 2013;Zhu and Tang, 2017). Because the most efficient pathway for succinate production is through the reductive branch of TCA cycle, most succinic acid production work would knock out the other pathways of mixed acid fermentation (Jantama et al, 2008), removal of PTS system for glucose uptake to conserve the PEP pool (Chatterjee et al, 2001), and overexpression of anaplerotic reactions (Millard et al, 1996;Stols et al, 1997).…”

Section: Di-acidsmentioning

confidence: 99%

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Escherichia coli as a host for metabolic engineering

Pontrelli

Chiu

Lan

et al. 2018

Metabolic Engineering

282

140

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Over the past century, Escherichia coli has become one of the best studied organisms on earth. Features such as genetic tractability, favorable growth conditions, well characterized biochemistry and physiology, and availability of versatile genetic manipulation tools make E. coli an ideal platform host for development of industrially viable productions. In this review, we discuss the physiological attributes of E. coli that are most relevant for metabolic engineering, as well as emerging techniques that enable efficient phenotype construction. Further, we summarize the large number of native and non-native products that have been synthesized by E. coli, and address some of the future challenges in broadening substrate range and fighting phage infection.

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Section: Crisprmentioning

confidence: 99%

Section: Crisprmentioning

confidence: 99%

Section: Di-acidsmentioning

confidence: 99%

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Escherichia coli as a host for metabolic engineering

Pontrelli

Chiu

Lan

et al. 2018

Metabolic Engineering

282

140

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“…At the same time, the by-product titers of succinic, lactic and acetic acid were dramatically decreased to 1.18, 1.08 and 0.48 g/L, respectively ( Zhang et al, 2011 ). In E. coli , all these by-products are derived from pyruvate, and inhibition of pyruvate formation is an effective strategy for reducing by-product biosynthesis and PEP consumption ( Zhu and Tang, 2017 ). For instance, deletion of pyruvate kinase ( pykA or pykF ) reduced lactate production by over 90% ( Zhang et al, 2011 ).…”

Section: Malic Acid Production By Genetically Engineered Microorganisms Using Metabolic Engineering Strategiesmentioning

confidence: 99%

Microbial Biosynthesis of L-Malic Acid and Related Metabolic Engineering Strategies: Advances and Prospects

Wei

et al. 2021

Front. Bioeng. Biotechnol.

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Malic acid, a four-carbon dicarboxylic acid, is widely used in the food, chemical and medical industries. As an intermediate of the TCA cycle, malic acid is one of the most promising building block chemicals that can be produced from renewable sources. To date, chemical synthesis or enzymatic conversion of petrochemical feedstocks are still the dominant mode for malic acid production. However, with increasing concerns surrounding environmental issues in recent years, microbial fermentation for the production of L-malic acid was extensively explored as an eco-friendly production process. The rapid development of genetic engineering has resulted in some promising strains suitable for large-scale bio-based production of malic acid. This review offers a comprehensive overview of the most recent developments, including a spectrum of wild-type, mutant, laboratory-evolved and metabolically engineered microorganisms for malic acid production. The technological progress in the fermentative production of malic acid is presented. Metabolic engineering strategies for malic acid production in various microorganisms are particularly reviewed. Biosynthetic pathways, transport of malic acid, elimination of byproducts and enhancement of metabolic fluxes are discussed and compared as strategies for improving malic acid production, thus providing insights into the current state of malic acid production, as well as further research directions for more efficient and economical microbial malic acid production.

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“…With the advantages of mild reaction conditions as well as being environmentally friendly, bio-based succinate production from renewable resources has attracted increasing attention [18]. Because of its simple gene manipulation and low nutrient requirements, E. coli was an excellent workhorse for succinate production [19]. There were three main pathways for succinate production, including the oxidative TCA pathway, the glyoxylate pathway, and the reductive TCA pathway, with different theoretical yields of 1, 1.25, and 1 mol mol −1 glucose, respectively [20].…”

Section: Introductionmentioning

confidence: 99%