Current advances in the functional studies of fatty acids and fatty acid-derived lipids inC. elegans

Abstract

Fatty acids and fatty acid-derived lipids (FAs/FADLs) play essential roles in many living organisms, including contributions to membrane structure and signaling transduction. Aberrant metabolism of FAs/FADLs often causes diseases and health problems. However, the detailed mechanistic studies of specific FAs/FADLs are limited. has been an effective model system for FA/ FADL studies due to its powerful genetics and conserved lipid biosynthetic pathways. The recently developed high-throughput analytic tools also enable sophisticated profiling of lipids molecules in , which is critical for understanding their specific functions. Here we review a subset of current advances in FA/FADL functional studies in.

Biogenic Amine Neurotransmitters Promote Eicosanoid Production And Protein Homeostasis

“…C. elegans contains a complete set of genes to produce n-6 (w6) and n-3 (w3) longchain PUFAs (Fig. 4C), including arachidonic acid (AA) and eicosapentaenoic acid (EPA), from oleic acid (OA) (Vrablik & Watts, 2013, Wallis, Watts et al, 2002, Ying & Zhu, 2016. We tested this hypothesis by collecting and lysing synchronized day 2 adult nematodes, followed by alkaline hydrolysis and LC-MS. AA-derived n-6 eicosanoids (Fig.…”

Biogenic amine neurotransmitters promote eicosanoid production and protein homeostasis

“…If DA, 5‐HT, and TA synthesis are required to promote the expression of CYP14, CYP29, and CYP34 genes, then do these biogenic amines promote the production of eicosanoids from PUFAs? C. elegans contains a complete set of genes to produce n ‐6 (ω6) and n ‐3 (ω3) long‐chain PUFAs (Fig 4C), including arachidonic acid (AA) and eicosapentaenoic acid (EPA), from oleic acid (OA) (Wallis et al , 2002; Watts & Browse, 2002; Vrablik & Watts, 2013; Ying & Zhu, 2016). We tested this hypothesis by collecting and lysing synchronized day 2 adult nematodes, followed by either direct LC‐MS analysis (for free fatty acid extraction) or alkaline hydrolysis and LC‐MS analysis (for total fatty acid extraction).…”