Current Advances in the Application of Raman Spectroscopy for Molecular Diagnosis of Cervical Cancer

Ramos, Inês; Malkin, Alison; Lyng, Fiona M.

doi:10.1155/2015/561242

Cited by 51 publications

(42 citation statements)

References 48 publications

(57 reference statements)

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“…All of this information can be used to help doctors to diagnose diseases, determine the appropriate treatment, and even evaluate prognosis. To date, Raman technology has been used to diagnose many cancers, including cervical cancer, malignant skin tumors, gastric cancer, colorectal cancer, and breast cancer [19][20][21][22]. This article aims to review the application of Raman spectra in breast cancer and describe the newest progress in this field.…”

Section: Brief Overview Of Raman Spectroscopymentioning

confidence: 99%

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

Gao

Han

et al. 2017

Journal of Spectroscopy

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Raman spectroscopy has been widely used as an important clinical tool for real-time in vivo cancer diagnosis. Raman information can be obtained from whole organisms and tissues, at the cellular level and at the biomolecular level. The aim of this paper is to review the newest developments of Raman spectroscopy in the field of breast cancer diagnosis and treatment. Raman spectroscopy can distinguish malignant tissues from noncancerous/normal tissues and can assess tumor margins or sentinel lymph nodes during an operation. At the cellular level, Raman spectra can be used to monitor the intracellular processes occurring in blood circulation. At the biomolecular level, surface-enhanced Raman spectroscopy techniques may help detect the biomarker on the tumor surface as well as evaluate the efficacy of anticancer drugs. Furthermore, Raman images reveal an inhomogeneous distribution of different compounds, especially proteins, lipids, microcalcifications, and their metabolic products, in cancerous breast tissues. Information about these compounds may further our understanding of the mechanisms of breast cancer.

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Section: Brief Overview Of Raman Spectroscopymentioning

confidence: 99%

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

Gao

Han

et al. 2017

Journal of Spectroscopy

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“…Among the possible applications of Raman micro spectroscopy in the clinical setting, cancer research and diagnostics remain among the most promising, including cancer detection and typing, for example oral , gastric , breast , cervical , skin cancers as well as therapeutic follow up and response to chemotherapeutic drugs .…”

Section: Introductionmentioning

confidence: 99%

Differentiating responses of lung cancer cell lines to Doxorubicin exposure: in vitro Raman micro spectroscopy, oxidative stress and bcl‐2 protein expression

Farhane¹,

Bonnier

Maher³

et al. 2016

Journal of Biophotonics

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The potential of Raman micro spectroscopy as an in vitro, non-invasive tool for clinical applications has been demonstrated in recent years, specifically for cancer research. To further illustrate its potential as a high content and label free technique, it is important to show its capability to elucidate drug mechanisms of action and cellular resistances. In this study, cytotoxicity assays were employed to establish the toxicity profiles for 24 hr exposure of lung cancer cell lines, A549 and Calu-1, to the commercially available drug, doxorubicin (DOX). Raman spectroscopy, coupled with Confocal Laser Scanning Microscopy and Flow Cytometry, was used to track the DOX mechanism of action, at a subcellular level, and to study the mechanisms of cellular resistance to DOX. Biomarkers related to the drug mechanism of action and cellular resistance to apoptosis, namely reactive oxygen species (ROS) and bcl-2 protein expression, respectively, were also measured and correlated to Raman spectral profiles. Calu-1 cells are shown to exhibit spectroscopic signatures of both direct DNA damage due to intercalation in the nucleus and indirect damage due to oxidative stress in the cytoplasm, whereas the A549 cell line only exhibits signatures of the former mechanism of action. PCA of nucleolar, nuclear and cytoplasmic regions of A549 and Calu-1 with corresponding loadings of PC1 and PC2.

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“…A recent review article on the application of Raman spectroscopy for cervical cancer presents an excellent summary of all relevant studies in a table. 167 Beyond the realm of cancer detection, our group has demonstrated the potential of Raman spectroscopy to track biochemical changes in the cervix during pregnancy and develop an understanding of cervical remodeling that occurs throughout pregnancy and parturition. 168,169 …”

Section: Clinical Applications Of Raman Spectroscopymentioning

confidence: 99%

Clinical instrumentation and applications of Raman spectroscopy

2016

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Clinical diagnostic devices provide new sources of information that give insight about the state of health which can then be used to manage patient care. These tools can be as simple as an otoscope to better visualize the ear canal or as complex as a wireless capsule endoscope to monitor the gastrointestinal tract. It is with tools such as these that medical practitioners can determine when a patient is healthy and to make an appropriate diagnosis when he/she is not. The goal of diagnostic medicine then is to efficiently determine the presence and cause of disease in order to provide the most appropriate intervention. The earliest form of medical diagnostics relied on the eye – direct visual observation of the interaction of light with the sample. This technique was espoused by Hippocrates in his 5th century BCE work Epidemics, in which the pallor of a patient’s skin and the coloring of the bodily fluids could be indicative of health. In the last hundred years, medical diagnosis has moved from relying on visual inspection to relying on numerous technological tools that are based on various types of interaction of the sample with different types of energy – light, ultrasound, radio waves, X-rays etc. Modern advances in science and technology have depended on enhancing technologies for the detection of these interactions for improved visualization of human health. Optical methods have been focused on providing this information in the micron to millimeter scale while ultrasound, X-ray, and radio waves have been key in aiding in the millimeter to centimeter scale. While a few optical technologies have achieved the status of medical instruments, many remain in the research and development phase despite persistent efforts by many researchers in the translation of these methods for clinical care. Of these, Raman spectroscopy has been described as a sensitive method that can provide biochemical information about tissue state while maintaining the capability of delivering this information in real-time, non-invasively, and in an automated manner. This review presents the various instrumentation considerations relevant to the clinical implementation of Raman spectroscopy and reviews a subset of interesting applications that have successfully demonstrated the efficacy of this technique for clinical diagnostics and monitoring in large (n ≥ 50) in vivo human studies.

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Current Advances in the Application of Raman Spectroscopy for Molecular Diagnosis of Cervical Cancer

Cited by 51 publications

References 48 publications

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

Differentiating responses of lung cancer cell lines to Doxorubicin exposure: in vitro Raman micro spectroscopy, oxidative stress and bcl‐2 protein expression

Clinical instrumentation and applications of Raman spectroscopy

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