Curing the Drug Resistance Plasmid in E. Coli O157:h7

Abstract

In this study, twenty five isolates of E. coli O157:H7 were identified among 200 samples taken from children under ten years old suffer from diarrhea. Isolates were identified from stool sample by using cultural, morphological, biochemical characteristics and serological test. The plasmid profile has been conducted by using gel electrophoresis. To control the antibiotic resistance of the tested E. coli O157:H7 isolates, curing of plasmid DNA was conducted using Ethidium bromide (EB) and elevated temperature (ET) at 46 °C. One of the most resistance isolate was chosen for this purpose E15 then treated with Ethidium bromide at 125 μg/ml. The results revealed that the genes encoded resistance to AK (Amikacin), CIP (Ciprofloxacin), CN (Gentamicin) and TMP + SXT (Trimethoprim + Sulphamethoxazole) (were cured from E15 and the percentage of curing was 28.57%. The results confirmed by conducting gel electrophoresis and showed that EB removed three plasmid of E15, while two plasmids remained. On the other hand, elevated temperature used also as curing agent for the same isolate and the results revealed that resistance to AK, CIP, CN and TMP + SXT genes were cured from E15 and the curing percent was 35.71% after incubating the isolate at 46 °C. The results confirmed by conducting gel electrophoresis and showed that tested isolate lost three plasmids after incubation at 46 °C.

The Curing Activity of 5-Fluorouracil (5-FU) and Its Role in Reducing The Antibiotic Sensitivity in Some Resistance Bacteria

“…The results of the current study showed variation in plasmid content between different isolates and sometimes between isolates of the same bacterial species , this variation in plasmid content may be due to the difference in both the source and geographical location of the isolate of the studied bacteria, as many factors play a role in the presence and transmission of plasmids in different bacterial species, as these plasmids are transmitted between different bacterial species and genera utilizing conjugation between bacterial cells [11]. Some multidrug-resistant bacteria contain plasmids of various molecular weights, while others do not contain plasmids, indicating that the latter's resistance was chromosomally carried [12].…”

Anti-Plasmid Activity of Chlorpromazine in Types of Antibiotics Resistant Pathogenic Bacteria

“…Increasing the consumption of antibiotics always leads to the emergence and proliferation of resistant bacteria [12]. As the bacteria acquire resistance to antibiotics either through chromosomal mutations or through horizontal gene transfer by mobile genetic elements including plasmids, resistant or Rplasmids in particular [19]. According to World Health Organization (WHO) the development and spread of ABSTRACT This study was performed to evaluate the ability of chlorpromazine compound to cure the plasmids and remove the antibiotic resistance character of multidrug resistance Gram-negative pathogenic bacteria.The curing experiment was done by incubating bacterial strains in nutrient broth with sub MIC concentration of chlorpromazine, and the disc diffusion method was used before and after curing to evaluate antibiotic resistance pattern of bacteria.…”