Curdlan production from cassava starch hydrolysates by Agrobacterium sp. DH-2

Wan, Jie; Shao, Zhiyu; Jiang, Deming; Gao, Hongliang; Yang, Xu-Zhao

doi:10.1007/s00449-022-02718-8

Cited by 6 publications

(3 citation statements)

References 48 publications

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“…Gel strength is a crucial indicator of the gelatin properties of curdlans. Typically, strain ATCC31749 cannot produce curdlan when using starch or dextrin as the substrate, which is consistent with the findings of Wan et al [27]. As shown in Figure 5, when the recombinant strain is cultured with 5% glucose and dextrin as substrates, curdlan yields are 29.38 ± 2.1 g/L and 30.40 ± 1.4 g/L, with gel strengths of 657.8 ± 21.6 and 703.5 ± 34.2 g/cm 2 , respectively.…”

Section: Curdlan Productions By Sp-amyaxccsupporting

confidence: 91%

“…Therefore, one of the current research hotspots in industrial curdlan production is the expansion of the carbon sources for fermentation. An example of this research is the work of Wan et al [27], who demonstrated the feasibility of Agrobacterium sp. to utilize cassava starch as a substrate for curdlan biosynthesis by supplementing exogenous β-amylase and pullulanase during fermentation.…”

Section: Amy-f2 Caatttcacacaggag-gaattcatgaaaaaactggtcatcmentioning

confidence: 99%

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Optimizing Curdlan Synthesis: Engineering Agrobacterium tumefaciens ATCC31749 for Enhanced Production Using Dextrin as a Carbon Source

Yu,

Wang,

Wang

et al. 2024

Fermentation

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A key goal in current research on industrial curdlan production is the expansion of carbon sources for fermentation. In this study, a recombinant bacterial strain, sp-AmyAXCC, capable of fermenting and synthesizing curdlan using dextrin as a carbon source, was produced via heterologous expression of IPTG-inducible α-amylase from Xanthomonas campestris NRRL B-1459 in Agrobacterium tumefaciens ATCC31749. External expression of the enzyme was confirmed by western blotting, and the expression levels of exogenous proteins during the fermentation process were monitored. Additionally, the properties of the curdlan product were characterized using attenuated total reflectance-Fourier transform infrared spectroscopy and X-ray diffraction. The recombinant strain produced curdlan at a titer of 30.40 ± 0.14 g/L, gel strength of 703.5 ± 34.2 g/cm2, and a molecular weight of 3.58 × 106 Da, which is 33% greater than the molecular weight of native curdlan (2.69 × 106 Da). In the batch fermentation of sp-AmyAXCC with 12% dextrin as a carbon source, the titer of curdlan was 66.7 g/L with a yield of 0.56 g/g, and a productivity rate of 0.62 g/L/h at 108 h. The results of this study expand the substrate spectrum for Agrobacterium fermentation in curdlan production and provides guidance for further industrialization of curdlan production.

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Section: Curdlan Productions By Sp-amyaxccsupporting

confidence: 91%

Section: Amy-f2 Caatttcacacaggag-gaattcatgaaaaaactggtcatcmentioning

confidence: 99%

Optimizing Curdlan Synthesis: Engineering Agrobacterium tumefaciens ATCC31749 for Enhanced Production Using Dextrin as a Carbon Source

Yu,

Wang,

Wang

et al. 2024

Fermentation

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“…These sugars can be obtained by hydrolysis of various renewable raw materials and biomass sources. Hydrolysis methods can be different, and various chemical agents and/or enzymes, including enzymatic complexes, can be used for these purposes [15,32,59,63,82,85].…”

Section: Features Of Btcs Determine the Palette Of Substrates (Types ...mentioning

confidence: 99%

Biocatalysts in Synthesis of Microbial Polysaccharides: Properties and Development Trends

et al. 2022

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Polysaccharides synthesized by microorganisms (bacterial cellulose, dextran, pullulan, xanthan, etc.) have a set of valuable properties, such as being antioxidants, detoxifying, structuring, being biodegradable, etc., which makes them suitable for a variety of applications. Biocatalysts are the key substances used in producing such polysaccharides; therefore, modern research is focused on the composition and properties of biocatalysts. Biocatalysts determine the possible range of renewable raw materials which can be used as substrates for such synthesis, as well as the biochemistry of the process and the rate of molecular transformations. New biocatalysts are being developed for participating in a widening range of stages of raw material processing. The functioning of biocatalysts can be optimized using the following main approaches of synthetic biology: the use of recombinant biocatalysts, the creation of artificial consortia, the combination of nano- and microbiocatalysts, and their immobilization. New biocatalysts can help expand the variety of the polysaccharides’ useful properties. This review presents recent results and achievements in this field of biocatalysis.

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Production of Curdlan by Agrobacterium sp. DH-2 Using Sugarcane Molasses-Based Medium

Wan

Gao

et al. 2023

J Polym Environ

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Curdlan production from cassava starch hydrolysates by Agrobacterium sp. DH-2

Cited by 6 publications

References 48 publications

Optimizing Curdlan Synthesis: Engineering Agrobacterium tumefaciens ATCC31749 for Enhanced Production Using Dextrin as a Carbon Source

Optimizing Curdlan Synthesis: Engineering Agrobacterium tumefaciens ATCC31749 for Enhanced Production Using Dextrin as a Carbon Source

Biocatalysts in Synthesis of Microbial Polysaccharides: Properties and Development Trends

Production of Curdlan by Agrobacterium sp. DH-2 Using Sugarcane Molasses-Based Medium

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