Curdlan-Conjugated PLGA Nanoparticles Possess Macrophage Stimulant Activity and Drug Delivery Capabilities

Functionalization of PLGA Nanoparticles with 1,3-β-glucan Enhances the Intracellular Pharmacokinetics of Rifampicin in Macrophages

“…An emulsion-solvent evaporation technique (12) was used to synthesize RIF loaded PLGA (PLGA(RIF)) and Glu-PLGA NPs (Glu-PLGA(RIF)). To synthesize PLGA(RIF) NPs, a 20 mg/ml PLGA solution in ethyl acetate (containing 2 mg/ml RIF) was added drop-wise to 10 mg/ml PVA solution in de-ionised water under stirring at 1000 rpm.…”

“…The human monocyte cell line THP-1 was cultured in RPMI 1640 medium supplemented with 10% fetal calf serum and 1% penicillin streptomycin antibiotic mixture in 6-well culture plates at 37°C, 5% CO 2 atmosphere (12). Prior to experiments, cells seeded at a density of 10 6 cells/well were induced to undergo terminal differentiation into macrophages by incubation in the presence of 20 nM of PMA for 24 h. Formation of macrophages was confirmed by microscopy.…”

“…In previous work, we conjugated the polysaccharide 1,3-β-glucan onto the polymer poly(lactic-coglycolic acid) (PLGA) and formulated glucan functionalized PLGA NPs. We reported that these NPs were not toxic to macrophages (12). 1,3-β-glucan is of use as a macrophage targeting ligand as it interacts with the Dectin-1 receptor; a receptor predominantly expressed on macrophage surfaces (13).…”

Biomedical potential of chitosan/HA and chitosan/β-1,3-glucan/HA biomaterials as scaffolds for bone regeneration — A comparative study

“…Thus, the β-1,3-glucan has also great potential in biomedical applications. So far, curdlan was used as effective drug carriers [12,14], and curdlan/chitosan blending membranes were prepared for antibacterial applications [15]. Nevertheless, apart from our previous papers there are no reports describing this particular β-1,3-glucan as a component of cell scaffolds for bone tissue engineering application.…”

Therapeutic implications of nano-encapsulated rifabutin, azithromycin & ethambutol against experimental Mycobacterium avium infection in mice

“…Poly (DL-lactide-co-glycolide) (PLGA) is an FDA-approved biodegradable and biocompatible drug carrier for human11. It is known to deliver antimicrobial drugs to treat various infectious diseases by improving bioavailability and pharmacokinetic profile of these agents1213. Encapsulation of antitubercular drugs has been reported to hold promise for better management of the disease1415.…”