Curd-Peptide Based Novel Hydrogel Inhibits Biofilm Formation, Quorum Sensing, Swimming Mortility of Multi-Antibiotic Resistant Clinical Isolates and Accelerates Wound Healing Activity

Manna, Sounik; Ghosh, Ananta K.; Mandal, Santi M.

doi:10.3389/fmicb.2019.00951

Cited by 21 publications

(9 citation statements)

References 32 publications

(40 reference statements)

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“…Manna, (2019) reported an antimicrobial peptide (AMP) with a sequence YLGYLEQLLRLK, corresponding to residues 106 to 117 of bovine α‐S1casein hydrolyzed by protease enzyme and named it BCp12. The BCp12 inhibited the growth of many bacteria and the minimum inhibitory concentration ranged from 0.8 to 1.6 mg/ml (Zhao et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Peptidomics‐based identification of an antimicrobial peptide derived from goat milk fermented by Lactobacillus rhamnosus ( C25 )

Iram

Kindarle

Sansi

et al. 2022

Journal of Food Biochemistry

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Antimicrobial peptides (AMPs) are emerging as promising novel drug applicants. In the present study, goat milk was fermented using Lactobacillus rhamnosus C25 to generate bioactive peptides (BAPs). The peptide fractions generated were separated using ultrafiltration membranes with molecular weight cut‐offs of 3, 5, and 10 kDa, and their antimicrobial activity toward Gram‐positive and Gram‐negative bacteria was investigated. Isolated AMPs were characterized using RP‐HPLC and identified by LC–MS/MS. A total of 569 sequences of peptides were identified by mass spectrometry. Out of the 569, 36 were predicted as AMPs, 21 were predicted as cationic, and out of 21, 6 AMPs were helical peptides. In silico analysis indicated that the majority of peptides were antimicrobial and cationic in nature, an important factor for peptide interaction with the negative charge membrane of bacteria. The results showed that the peptides of <5 kDa exhibited maximum antibacterial activity against E. faecalis, E. coli, and S. typhi. Further, molecular docking was used to evaluate the potent MurD ligase inhibitors. On the basis of ligand binding energy, six predicted AMPs were selected and then analyzed by AutoDock tools. Among the six AMPs, peptides IGHFKLIFSLLRV (−7.5 kcal/mol) and KSFCPAPVAPPPPT (−7.6 kcal/mol), were predicted as a high‐potent antimicrobial. Based on these findings, in silico investigations reveal that proteins of goat milk are a potential source of AMPs. This is for the first time that the antimicrobial peptides produced by Lactobacillus rhamnosus (C25) fermentation of goat milk have been identified via LC–MS/MS and predicted as AMPs, cationic charges, helical structure in nature, and potent MurD ligase inhibitors. These peptides can be synthesized and improved for use as antimicrobial agents. Practical applications Goat milk is considered a high‐quality source of milk protein. According to this study, goat milk protein is a potential source of AMPs, Fermentation can yield goat milk‐derived peptides with a broad antibacterial activity spectrum at a low cost. The approach described here could be beneficial in that the significant AMPs can be synthesized and used in the pharmaceutical and food industries.

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Section: Discussionmentioning

confidence: 99%

Peptidomics‐based identification of an antimicrobial peptide derived from goat milk fermented by Lactobacillus rhamnosus ( C25 )

Iram

Kindarle

Sansi

et al. 2022

Journal of Food Biochemistry

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“…Since a substantial change in volume is usually not observed, HGs are also suitable as injectable vehicles ( Manna et al, 2019 ). Moreover, HG can also polymerize in situ becoming a shear-thinning material after injection, allowing, therefore, AMP delivery.…”

Section: Drug Development and Formulation Approaches For Amp Applicationsmentioning

confidence: 99%

Antimicrobial Peptides: A New Hope in Biomedical and Pharmaceutical Fields

Moretta

Scieuzo

Petrone

et al. 2021

Front. Cell. Infect. Microbiol.

236

178

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Antibiotics are essential drugs used to treat pathogenic bacteria, but their prolonged use contributes to the development and spread of drug-resistant microorganisms. Antibiotic resistance is a serious challenge and has led to the need for new alternative molecules less prone to bacterial resistance. Antimicrobial peptides (AMPs) have aroused great interest as potential next-generation antibiotics, since they are bioactive small proteins, naturally produced by all living organisms, and representing the first line of defense against fungi, viruses and bacteria. AMPs are commonly classified according to their sources, which are represented by microorganisms, plants and animals, as well as to their secondary structure, their biosynthesis and their mechanism of action. They find application in different fields such as agriculture, food industry and medicine, on which we focused our attention in this review. Particularly, we examined AMP potential applicability in wound healing, skin infections and metabolic syndrome, considering their ability to act as potential Angiotensin-Converting Enzyme I and pancreatic lipase inhibitory peptides as well as antioxidant peptides. Moreover, we argued about the pharmacokinetic and pharmacodynamic approaches to develop new antibiotics, the drug development strategies and the formulation approaches which need to be taken into account in developing clinically suitable AMP applications.

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“…Nonetheless, hydrogels prepared from natural extracts can effectively solve this problem. [ 212 ] A Stanford professor partnered with experts in chitosan's hemostatic antibacterial properties that were produced by Schiff's base reaction regarding the ability to integrate and organize well. They developed a kind of based on the in situ imine light response of the crosslinked chitosan hydrogel liquid bandage (LBA) used to deal with the emergency wound under UV irradiation and the modified carboxymethyl chitosan (CMC) of the neighboring nitrobenzene into neighboring nitrobenzene formaldehyde.…”

Section: Animal Extracts For Antibacterial Applicationsmentioning

confidence: 99%

Natural Extracts for Antibacterial Applications

Chen,

Mao

et al. 2023

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Bacteria‐induced epidemics and infectious diseases are seriously threatening the health of people around the world. In addition, antibiotic therapy has been inducing increasingly more serious bacterial resistance, which makes it urgent to develop new treatment strategies to combat bacteria, including multidrug‐resistant bacteria. Natural extracts displaying antibacterial activity and good biocompatibility have attracted much attention due to greater concerns about the safety of synthetic chemicals and emerging drug resistance. These antibacterial components can be isolated and utilized as antimicrobials, as well as transformed, combined, or wrapped with other substances by using modern assistive technologies to fight bacteria synergistically. This review summarizes recent advances in natural extracts from three kinds of sources—plants, animals, and microorganisms—for antibacterial applications. This work discusses the corresponding antibacterial mechanisms and the future development of natural extracts in antibacterial fields.

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Curd-Peptide Based Novel Hydrogel Inhibits Biofilm Formation, Quorum Sensing, Swimming Mortility of Multi-Antibiotic Resistant Clinical Isolates and Accelerates Wound Healing Activity

Cited by 21 publications

References 32 publications

Peptidomics‐based identification of an antimicrobial peptide derived from goat milk fermented by Lactobacillus rhamnosus ( C25 )

Peptidomics‐based identification of an antimicrobial peptide derived from goat milk fermented by Lactobacillus rhamnosus ( C25 )

Antimicrobial Peptides: A New Hope in Biomedical and Pharmaceutical Fields

Natural Extracts for Antibacterial Applications

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