Curcuminoids-loaded liposomes: influence of lipid composition on their physicochemical properties and efficacy as delivery systems

Battista, Sara; Maggi, Maria Anna; Bellio, Pierangelo; Galantini, Luciano; D’Archivio, Angelo Antonio; Celenza, Giuseppe; Colaiezzi, Roberta; Giansanti, Luisa

doi:10.1016/j.colsurfa.2020.124759

Cited by 20 publications

(16 citation statements)

References 23 publications

(23 reference statements)

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“…Interestingly, the antimicrobial properties of MOX-loaded PC/Chol (1:1) liposomes (DRV and AL) towards S. epidermidis planktonic and biofilm bacteria were demonstrated to be significantly different; the AL liposomes demonstrated increased and prolonged antimicrobial effects, as compared to the DRV liposomes, whereas both liposome types performed better than free MOX, which was in good agreement with other studies [30,32,47,48,[52][53][54][55][56].…”

Section: Discussionsupporting

confidence: 90%

“…Other studies have also reported enhanced activity of liposomal drugs (compared to free drugs) towards planktonic bacteria. In one case, curcumin antibacterial properties were augmented by encapsulation in positive liposomes, and the importance of physicochemical properties of liposomes (bilayer rigidity and liposome charge) as crucial parameters that determine their interaction with the biological environment was pointed out [48]. In another report, the antibacterial properties of cephalexin-loaded niosomes (a type of liposomes that includes surfactants in their membrane) against S. aureus (Gram-positive) and Escherichia coli (Gram-negative) bacteria were augmented (MIC was reduced by two or four times) compared to the efficiency of the free drug [30].…”

Section: Effect Of Liposome Preparation Methods On Antimicrobial Acti...mentioning

confidence: 99%

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Moxifloxacin Liposomes: Effect of Liposome Preparation Method on Physicochemical Properties and Antimicrobial Activity against Staphylococcus epidermidis

et al. 2022

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The aim of this study was the development of optimal sustained-release moxifloxacin (MOX)-loaded liposomes as intraocular therapeutics of endophthalmitis. Two methods were compared for the preparation of MOX liposomes; the dehydration–rehydration (DRV) method and the active loading method (AL). Numerous lipid-membrane compositions were studied to determine the potential effect on MOX loading and retention in liposomes. MOX and phospholipid contents were measured by HPLC and a colorimetric assay for phospholipids, respectively. Vesicle size distribution and surface charge were measured by DLS, and morphology was evaluated by cryo-TEM. The AL method conferred liposomes with higher MOX encapsulation compared to the DRV method for all the lipid compositions used. Cryo-TEM showed that both liposome types had round vesicular structure and size around 100−150 nm, while a granular texture was evident in the entrapped aqueous compartments of most AL liposomes, but substantially less in DRV liposomes; X-ray diffraction analysis demonstrated slight crystallinity in AL liposomes, especially the ones with highest MOX encapsulation. AL liposomes retained MOX for significantly longer time periods compared to DRVs. Lipid composition did not affect MOX release from DRV liposomes but significantly altered drug loading/release in AL liposomes. Interestingly, AL liposomes demonstrated substantially higher antimicrobial potential towards S. epidermidis growth and biofilm susceptibility compared to corresponding DRV liposomes, indicating the importance of MOX retention in liposomes on their activity. In conclusion, the liposome preparation method/type determines the rate of MOX release from liposomes and modulates their antimicrobial potential, a finding that deserves further in vitro and in vivo exploitation.

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Section: Discussionsupporting

confidence: 90%

Section: Effect Of Liposome Preparation Methods On Antimicrobial Acti...mentioning

confidence: 99%

Moxifloxacin Liposomes: Effect of Liposome Preparation Method on Physicochemical Properties and Antimicrobial Activity against Staphylococcus epidermidis

et al. 2022

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“…A considerable drop in the ζ potential value after dialysis is evident only in the case of liposomes formulated with GL4, suggesting that this amphiphile is partially lost during liposome purification. This is not surprising given that GL4 is more soluble than the other glycosylated amphiphiles and displays a higher cmc value (4.3 × 10 − 3 M) [26].…”

Section: Size ζ Potential and Rsv Localization Of Liposomesmentioning

confidence: 90%

“…In order to assess the antioxidant power of RSV-loaded liposomes, the Total Antioxidative Capacity (TAC) of a sample (from Ozcan Erel [25][26][27]) was evaluated. The assay was based on the formation of the radical cation 2,2 ′ -azinobis-[3-ethylbenzothiazoline-6-sulfonate] (ABTS+•, with a characteristic petrol green colour) through oxidation of the neutral, colourless 2,2 ′ -azinobis-[3-etilbenzotiazolin-6-sulfonic] acid (ABTS) by hydrogen peroxide.…”

Section: Evaluation Of Anti-oxidant Properties Of Rsv-loaded Liposomes (Abts Assay)mentioning

confidence: 99%

“…In view of this, it is important to assess to what extent the inclusion of RSV in lipid bilayers could protect it from oxidative degradation. The assay used to evaluate the antioxidant capacity of RSV loaded in liposomes was the method reported by Ozcan Erel [26] to assess the Total Antioxidative Capacity (TAC) of a sample. The cation radical (ABTS +• ), produced by oxidation of ABTS with hydrogen peroxide, is stable at pH 3.6 for up to 6 months [21] at 4 • C, but it decomposes rapidly to its neutral ABTS form at higher pH values.…”

Section: Evaluation Of the Anti-oxidant Properties Of Rsv-loaded Liposomesmentioning

confidence: 99%

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Mannosyl, glucosyl or galactosyl liposomes to improve resveratrol efficacy against Methicillin Resistant Staphylococcus aureus biofilm

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Pagano

Ceccacci

et al. 2021

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Novel cationic glycoliposomes, composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), cholesterol (Chol) and glycoamphiphiles featuring a galactosyl, mannosyl or glucosyl moiety have been investigated for the targeted delivery of trans-resveratrol (RSV), a Quorum Sensing Inhibitor (QSI), to Methicillin Resistant Staphylococcus Aureus (MRSA) biofilms.All the glycosylated formulations show a 10-20 % reduction of their hydrodynamic size and a high positive increase in ζ-potential (20÷27mV), with respect to the almost neutral DOPC/Chol liposomes (-3.7 mV). RSV is entrapped in liposomes with high Entrapment Efficiency (EE%), the formulations containing glycosylated amphiphiles showing higher values of EE% (79-90 %) than those containing DOPC/CHOL (65 %). In all the liposomal formulations, the inclusion of RSV causes a decrease in ζ-potential, which is particularly evident in the negative value of DOPC/Chol liposomes (-14.8 mV). This is probably due to the ionization of a small percentage of RSV molecules that point towards the lipid/water interface, as reported in the literature.Greater antioxidant activity is found when RSV is embedded in glycosylated liposomes, rather than DOPC/ CHOL liposomes. This finding suggests a different RSV distribution in the lipid membrane enclosing the glycosylated amphiphiles, which favor an external exposure of RSV.Biological assays carried out to monitor the demolition effect of RSV-loaded liposomes on mature biofilm of MRSA show that the presence of cationic glycoamphiphiles is essential for a demolition effect to take place on the biofilm matrix. In particular, RSV-galactosylated liposomes are the most effective in destroying MRSA biofilm even at a RSV concentration (0.019 mM) sixty times lower than the MIC (1.2 mM). This work demonstrates, for the first time, how the functionalization of liposomes with cationic glycosydic residues can enhance liposome performances as QSI nanocarriers for the treatment of biofilm associated infections.

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Correlation of Physicochemical and Antimicrobial Properties of Liposomes Loaded with (+)‐Usnic Acid

et al. 2020

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+)-Usnic acid (UA) is a natural substance that displays pharmacological activity, but it is barely soluble in water, so it was included in liposomes in order to study its properties. First, the effects of phospholipid structure and loading methodology on UA entrapment efficacy were evaluated. Then, the physicochemical and biological properties (UA delivery efficacy to Staphylococcus aureus bacterial cells) of different liposome formulations containing structurally related amphiphiles derived from L-prolinol were fully investigated. Entrapment efficiency of UA with passive loading by incubation was 80-100 molar percentage, which is related to lipophilicity of the drug and to the packing and fluidity of the bilayer. Some of the investigated formulations show the potential of UA in delivery systems (minimum inhibitory concentration of liposomal UA: 8 μg/mL) and even subtle variations of the molecular structure of lipids can significantly affect the liposomes' physicochemical properties and efficiency of drug release. μL of liposomes not previously incubated with bacteria (blank sample) and 2970 μL of Mueller-Hinton medium used in biological experiments.

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Curcuminoids-loaded liposomes: influence of lipid composition on their physicochemical properties and efficacy as delivery systems

Cited by 20 publications

References 23 publications

Moxifloxacin Liposomes: Effect of Liposome Preparation Method on Physicochemical Properties and Antimicrobial Activity against Staphylococcus epidermidis

Moxifloxacin Liposomes: Effect of Liposome Preparation Method on Physicochemical Properties and Antimicrobial Activity against Staphylococcus epidermidis

Mannosyl, glucosyl or galactosyl liposomes to improve resveratrol efficacy against Methicillin Resistant Staphylococcus aureus biofilm

Correlation of Physicochemical and Antimicrobial Properties of Liposomes Loaded with (+)‐Usnic Acid

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