Curcumin suppresses PPARδ expression and related genes in HT-29 cells

Wang, Jinbo; Li, Qi; Zheng, Shui Di; Wang, Heng-Zheng; Wu, Tingfeng

doi:10.3748/wjg.15.1346

Cited by 23 publications

(13 citation statements)

References 34 publications

(49 reference statements)

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“…2C, top) and, to a lesser extent, in HT29 cells (Fig. 2C, lower) and the corresponding pro- tein levels of the three PPARs in the two cell lines (39)(40)(41)(42)(43). Upon incubation with rosiglitazone, a time-and dose-dependent increase in ANGPTL4 protein was observed in T84 cells ( Fig.…”

Section: Angptl4 Production Is Stimulated By Short-chain Fatty Acidsmentioning

confidence: 91%

Short-Chain Fatty Acids Stimulate Angiopoietin-Like 4 Synthesis in Human Colon Adenocarcinoma Cells by Activating Peroxisome Proliferator-Activated Receptor γ

Alex

Lange

Amolo

et al. 2013

Molecular and Cellular Biology

225

140

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Section: Angptl4 Production Is Stimulated By Short-chain Fatty Acidsmentioning

confidence: 91%

Short-Chain Fatty Acids Stimulate Angiopoietin-Like 4 Synthesis in Human Colon Adenocarcinoma Cells by Activating Peroxisome Proliferator-Activated Receptor γ

Alex

Lange

Amolo

et al. 2013

Molecular and Cellular Biology

225

140

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“…The expression of IκB and MyD88 was analyzed by Western blot according to the previous method (Wang et al 2009). …”

Section: Western Blotmentioning

confidence: 99%

The inflammation regulation effects ofEnterococcus faeciumHDRsEf1 on human enterocyte-like HT-29 cells

Tian

Yang

et al. 2016

Animal Cells and Systems

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Enterococcus faecium HDRsEf1 strain used as a probiotic to inhibit intestine inflammation and improve animal growth performance has been proved by our research team; however, it remains unclear how HDRsEf1 was recognized by intestine cells and how it activates the downstream pathway which benefit intestine health. In this study, HDRsEf1 was used to stimulate HT-29 cell line to partially uncover the intestine benefit mechanism of HDRsEf1. The results of cell viability assays showed that HDRsEf1 had no toxicity on HT-29 at concentrations up to 1 × 10 8 CFU/mL, HDRsEf1 could upregulate the TLR1, TLR2, and TLR6 mRNA level, especially TLR2, and significantly downregulate the mRNA level of TLR4, TLR5, TLR7, TLR8, but did not significantly affect the mRNA or protein level of MyD88, which suggests that HDRsEf1 activates the TLR2 pathway in an MyD88-independent pattern. HDRsEf1 could significantly downregulate the mRNA level of pro-inflammatory factors IL-1β, IL-6, IL-8, IL-12p35, IL-17, and TNF-α and did not affect the anti-inflammatory factors IL-10, PPAR-γ, and TSLP; besides HDRsEf1 did not upregulate the degradation of IκB in HT-29 cells. By contrast, enterohemorrhagic E. coli (EHEC) O157:H7 strongly up-regulated the mRNA level of pro-inflammatory factors IL-1β, IL-6, IL-8, IL-23, and TNF-α, downregulated obviously anti-inflammatory factor PPAR-ɣ, and obviously upregulated the degradation of IκB, which suggested that HDRsEf1 may act as an antagonist to regulate intestine inflammation response to intestine pathogen. These findings shed a light on the intestine benefit mechanism of HDRsEf1. ARTICLE HISTORY

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“…butyricum MIYAIRI II 588 used in this study was obtained from Miyarisan Pharmaceutical Co. Ltd., Tokyo, Japan. This strain, originally isolated from soil, is a butyric-acid produce, spore-forming and gram-positive rod bacterium (Wang et al 2009). It was cultured in MRS broth at 288C in an anoxic environment.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 98%

“…A semiquantitative RT-PCR method was used to measure the expression levels of TLR2, TLR4, TLR5, TLR9, NF-kB, MyD88, IL-6, IL-8, IL-10, tumor necrosis factor alpha (TNF-a), and Hsp70 (Wang et al 2009). The primer sequences are shown in Table 1.…”

Section: Rt-pcr Analysismentioning

confidence: 99%

Immunomodulatory effects ofClostridium butyricumon human enterocyte-like HT-29 cells

Gao¹,

Qi²,

Tian-xing³

et al. 2013

Animal Cells and Systems

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Oral administration of Clostridium butyricum as probiotics is increasingly gaining importance in the treatment of intestinal inflammations and improvement of animal performance. The mechanisms of host cell receptor recognition of C. butyricum and downstream immune signaling pathways leading to this beneficial event, however, remain unclear. In this study, C. butyricum was investigated for its capability to influence the innate immune response of HT-29 cells to explore its mechanism of action. Our data showed that C. butyricum was able to stimulate toll-like receptor 2 (TLR2) production at mRNA level; however, TLR4, TLR5, TLR9, and myeloid differentiation primary response protein 88 (MyD88) transcription levels remain unaltered. The nuclear factor kB (NF-kB), interleukin-8 (IL-8), and tumor necrosis factor alpha (TNF-a) levels in response to C. butyricum were significantly increased, indicating that HT-29 cells were sensitised by C. butyricum. It is the first time that our findings have showed the specific signaling pathways in HT-29 cells stimulated by C. butyricum, which, at least in part, can help to explain the beneficial properties of C. butyricum.

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Curcumin suppresses PPARδ expression and related genes in HT-29 cells

Cited by 23 publications

References 34 publications

Short-Chain Fatty Acids Stimulate Angiopoietin-Like 4 Synthesis in Human Colon Adenocarcinoma Cells by Activating Peroxisome Proliferator-Activated Receptor γ

Short-Chain Fatty Acids Stimulate Angiopoietin-Like 4 Synthesis in Human Colon Adenocarcinoma Cells by Activating Peroxisome Proliferator-Activated Receptor γ

The inflammation regulation effects ofEnterococcus faeciumHDRsEf1 on human enterocyte-like HT-29 cells

Immunomodulatory effects ofClostridium butyricumon human enterocyte-like HT-29 cells

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