Curcumin stimulates exosome/microvesicle release in an in vitro model of intracellular lipid accumulation by increasing ceramide synthesis

García-Seisdedos, David; Babiy, Bohdan; Lerma, Milagros; Casado, María E.; Martı́nez-Botas, Javier; Lasunción, Miguel A.; Pastor, Óscar; Busto, Rebeca

doi:10.1016/j.bbalip.2020.158638

Cited by 18 publications

(21 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For therapeutic purposes, not only strategies for inhibition of exosome biogenesis, release or uptake have been proposed, but also mechanisms to improve lipid trafficking by enhancing exosome production and release. In this sense, curcumin, a hydrophobic polyphenol, seems to increase exosome secretion in C6 glial cells by stimulating the synthesis of ceramide, thereby decreasing the lipid concentration in the endo‐lysosomal compartment 140 . Therefore, this effect of curcumin over ceramide synthesis and exosome secretion could ameliorate the endo‐lysosomal lipid accumulation observed in lysosomal storage disorders.…”

Section: Exosome Lipids As Therapeutic Targetsmentioning

confidence: 99%

“…In this sense, curcumin, a hydrophobic polyphenol, seems to increase exosome secretion in C6 glial cells by stimulating the synthesis of ceramide, thereby decreasing the lipid concentration in the endo‐lysosomal compartment. 140 Therefore, this effect of curcumin over ceramide synthesis and exosome secretion could ameliorate the endo‐lysosomal lipid accumulation observed in lysosomal storage disorders.…”

Section: Exosome Lipids As Therapeutic Targetsmentioning

confidence: 99%

See 1 more Smart Citation

The role of lipids in exosome biology and intercellular communication: Function, analytics and applications

Donoso‐Quezada

Ayala‐Mar

González‐Valdez

2021

Traffic

136

View full text Add to dashboard Cite

Exosomes are extracellular vesicles that in recent years have received special attention for their regulatory functions in numerous biological processes. Recent evidence suggests a correlation between the composition of exosomes in body fluids and the progression of some disorders, such as cancer, diabetes and neurodegenerative diseases. In consequence, numerous studies have been performed to evaluate the composition of these vesicles, aiming to develop new biomarkers for diagnosis and to find novel therapeutic targets. On their part, lipids represent one of the most important components of exosomes, with important structural and regulatory functions during exosome biogenesis, release, targeting and cellular uptake. Therefore, exosome lipidomics has emerged as an innovative discipline for the discovery of novel lipid species with biomedical applications. This review summarizes the current knowledge about exosome lipids and their roles in exosome biology and intercellular communication. Furthermore, it presents the state‐of‐the‐art analytical procedures used in exosome lipidomics while emphasizing how this emerging discipline is providing new insights for future applications of exosome lipids in biomedicine.

show abstract

Section: Exosome Lipids As Therapeutic Targetsmentioning

confidence: 99%

Section: Exosome Lipids As Therapeutic Targetsmentioning

confidence: 99%

The role of lipids in exosome biology and intercellular communication: Function, analytics and applications

Donoso‐Quezada

Ayala‐Mar

González‐Valdez

2021

Traffic

136

View full text Add to dashboard Cite

show abstract

“…( Figure 6 ) [ 56 ]. Notably, curcumin increases ceramide concentration by stimulating de novo synthesis of ceramide, activating neutral sphingomyelinase and inhibiting the activity of sphingomyelin synthase [ 57 ]. Lloyd-Evans et al observed that curcumin reduces the intracellular accumulation of So, sphingomyelin, glycosphingolipids, and cholesterol by restoring the intracellular calcium content.…”

Section: Detoxification Of Fumonisins Using Biological Antioxidantsmentioning

confidence: 99%

Toxic Mechanism and Biological Detoxification of Fumonisins

Wang

et al. 2022

Toxins

View full text Add to dashboard Cite

Food safety is related to the national economy and people’s livelihood. Fumonisins are widely found in animal feed, feed raw materials, and human food. This can not only cause economic losses in animal husbandry but can also have carcinogenicity or teratogenicity and can be left in animal meat, eggs, and milk which may enter the human body and pose a serious threat to human health. Although there are many strategies to prevent fumonisins from entering the food chain, the traditional physical and chemical methods of mycotoxin removal have some disadvantages, such as an unstable effect, large nutrient loss, impact on the palatability of feed, and difficulty in mass production. As a safe, efficient, and environmentally friendly detoxification technology, biological detoxification attracts more and more attention from researchers and is gradually becoming an accepted technique. This work summarizes the toxic mechanism of fumonisins and highlights the advances of fumonisins in the detoxification of biological antioxidants, antagonistic microorganisms, and degradation mechanisms. Finally, the future challenges and focus of the biological control and degradation of fumonisins are discussed.

show abstract

“…Several lipids and lipid-metabolizing enzymes have been shown to play roles in the formation and release of exosomes/microvesicles [ 5 , 6 ]. In particular, ceramide appears to be essential for exosome/microvesicle secretion [ 7 , 8 , 9 , 10 , 11 ]. Ceramide is a coned-shaped lipid that promotes the coalescence of membrane domains and induces the spontaneous curvature of membranes, promoting domain-induced budding [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…We previously reported that curcumin, a hydrophobic polyphenol found in the rhizomes of Curcuma longa , stimulated exosome/microvesicle release via an increase in ceramide, allowing the export of lipids out of the cell, and therefore ameliorating the endo/lysosomal lipid accumulation found in lysosomal storage disorders [ 10 ]. Rottlerin is the principal polyphenol component of the Kamala plant, and is the red powder that covers the fruits of Mallotus philippinensis (Euphorbiaceae), an evergreen tree that grows in the tropical regions of Southeast Asia [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Rottlerin Stimulates Exosome/Microvesicle Release Via the Increase of Ceramide Levels Mediated by Ampk in an In Vitro Model of Intracellular Lipid Accumulation

et al. 2022

Self Cite

View full text Add to dashboard Cite

Exosomes/microvesicles originate from multivesicular bodies that allow the secretion of endolysosome components out of the cell. In the present work, we investigated the effects of rottlerin, a polyphenol, on exosome/microvesicle secretion in a model of intracellular lipid trafficking impairment, and elucidated the mechanism of action. In a model of lipid trafficking impairment in C6 glia cells, rottlerin increased ceramide levels, while decreasing hexosylceramide content. This was accompanied by increased exosome/microvesicle secretion, thereby reducing the concentration of lipids in the endolysosomal compartment. The reduction of hexosylceramide levels by rottlerin was attributed to the increase of β-glucosidase (glucosylceramidase) activity, and the effects of rottlerin were abrogated by β-glucosidase inhibitors such as isofagomine D-tartrate and AMP-deoxynojirimycin. Moreover, treatment with ML-266, a potent activator of the β-glucosidase enzyme, recapitulated the effects of rottlerin on the sphingolipid profile and exosome/microvesicle secretion. Finally, inhibition of AMPK (AMP-activated protein kinase) using compound C prevented both exosome/microvesicle secretion and the elimination of endolysosome lipids, which were promoted by rottlerin. The results showed that the decrease in intracellular lipid deposition induced by rottlerin was mediated by β-glucosidase activation and exosome/microvesicle release via the AMPK pathway. Rottlerin consumption could represent an additional health benefit in lysosomal deposition diseases.

show abstract

Curcumin stimulates exosome/microvesicle release in an in vitro model of intracellular lipid accumulation by increasing ceramide synthesis

Cited by 18 publications

References 42 publications

The role of lipids in exosome biology and intercellular communication: Function, analytics and applications

The role of lipids in exosome biology and intercellular communication: Function, analytics and applications

Toxic Mechanism and Biological Detoxification of Fumonisins

Rottlerin Stimulates Exosome/Microvesicle Release Via the Increase of Ceramide Levels Mediated by Ampk in an In Vitro Model of Intracellular Lipid Accumulation

Contact Info

Product

Resources

About