Curcumin Protects Rat Myocardium Against Isoproterenol-Induced Ischemic Injury: Attenuation of Ventricular Dysfunction Through Increased Expression of Hsp27 Alongwith Strengthening Antioxidant Defense System

Abstract

This investigation examines the role of heat shock protein (Hsp) 27 and its modulation by curcumin in isoproterenol-induced myocardial ischemic injury in rats. Evidence from hemodynamic functions and oxidative stress parameters were also included in the study. The animals were divided into control, isoproterenol, and curcumin 100, 200, and 400 mg/kg treatment groups. Curcumin was administered orally for 15 days to all the treated groups. On 13th and 14th day, isoproterenol (85 mg/kg, s.c.) was injected to curcumintreated and isoproterenol group. On day 15, hemodynamic parameters were recorded. Thereafter, animals were sacrificed and hearts were kept for biochemical and Western blot analysis. We found dosedependent increase in the expression of Hsp27 with drastic fall at highest dose. Hemodynamically, the lower 2 doses also restored the cardiac function as evident by improved contractile functions, decreased left ventricular end-diastolic pressure, restored arterial pressures, and heart rate. In addition, there was an increase in then level of superoxide dismutase, catalase, reduced glutathione, and decreased production of thiobarbituric acid reactive substances and leakage of cardiac necroenzyme creatine kinase-MB isoenzyme and lactate dehydrogenase in curcumin 100 and 200 mg/kg group as compared with isoproterenol. However, at a dose of 400 mg/kg, there was ineffectual protection against isoproterenol-induced myocardial damage. Our results suggested 200 mg/mg as the most optimum therapeutic dose showing improved cardiac function due to stabilization of cytoskeleton structure which in turn is attributed to Hsp27 expression along with fortified antioxidant defense system.

Antioxidant and vascular protective effects of curcumin and tetrahydrocurcumin in rats with l-NAME-induced hypertension

“…Tanwar et al (2010) demonstrated that CUR protected the heart from ischemic damage and restored cardiac functions by strengthening of the antioxidant defense system (Tanwar et al 2010). In our study, we found that the abilities of CUR or THU to improve hemodynamic status and restore vascular responsiveness are concomitant with the recoveries of eNOS protein expression in aortic tissue and plasma nitrate/nitrite level.…”

Protective effects of curcumin, vitamin C, or their combination on cadmium-induced hepatotoxicity

“…Curcumin has been reported to attenuate liver injury induced by diverse hepatotoxicants [39,40,41] via multiple mechanisms. The two doses of curcumin selected in this study were based on previous studies in which curcumin showed protective effect against oxidant damage in the heart [42], liver [43], and kidney [44]. Its efficacy in protecting against liver injury has been established by its antioxidant or free radical-scavenger action.…”

A randomized controlled trial on the anti-inflammatory effects of curcumin in patients with chronic sulphur mustard-induced cutaneous complications

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