Curcumin Protects an SH-SY5Y Cell Model of Parkinson’s Disease Against Toxic Injury by Regulating HSP90

Sang, Qiuling; Liu, Xiaoyang; Wang, Libo; Qi, Ling; Sun, Wei; Wang, Weiyao; Sun, Yuxin; Zhang, Haina

doi:10.1159/000495326

Cited by 37 publications

(22 citation statements)

References 24 publications

(34 reference statements)

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“…We found that a-cyperone upregulated Bcl-2 and downregulated Bax in H 2 O 2 -treated SH-SY5Y cells, suggesting that a-cyperone suppressed mitochondrial dysfunction. Sanga et al have proved that the inhibition of cleaved-caspase-3 production protects a SH-SY5Y against toxic injury (Sang et al, 2018). In addition, Wu et al have reported that carnosic acid protects against 6-hydroxydopamine-induced neurotoxicity in in vivo and in vitro models of PD, by inducing antioxidative enzymes and inhibiting the production of cleavedcaspase-3 (Wu et al, 2015).…”

Section: A B C D Ementioning

confidence: 99%

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

Huang

Liu

et al. 2020

Front. Pharmacol.

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a-Cyperone, extracted from Cyperus rotundus, has been reported to inhibit microgliamediated neuroinflammation. Oxidative stress and apoptosis play crucial roles in the course of Parkinson's disease (PD). PD is a common neurodegenerative disease characterized by selective death of dopaminergic neurons. This study was designed to investigate the neuroprotective effects of a-cyperone against hydrogen peroxide (H 2 O 2)induced oxidative stress and apoptosis in dopaminergic neuronal SH-SY5Y cells. Neurotoxicity was assessed by MTT assay and the measurement of lactic dehydrogenase (LDH) release. The level of reactive oxygen species (ROS) was measured by dichlorodihydrofluorescin diacetate (DCFH-DA) staining. The apoptosis of SH-SY5Y cells was evaluated by annexin-V-FITC staining. The translocation of NF-E2related factor 2 (Nrf2) was determined by western blot and immunofluorescence staining. Western blot analysis was conducted to determine the expression level of cleavedcaspase-3, the pro-apoptotic factor Bax, and the anti-apoptotic factor, Bcl-2. The results showed that a-cyperone substantially decreased H 2 O 2-induced death, release of LDH, and the production of ROS in SH-SY5Y cells. In addition, we found that a-cyperone attenuated H 2 O 2-induced cellular apoptosis. Moreover, a-cyperone remarkably reduced the expression of cleaved-caspase-3 and Bax, and upregulated Bcl-2. Furthermore, acyperone enhanced the nuclear translocation of Nrf2. Pretreatment with brusatol (BT, an Nrf2 inhibitor) attenuated a-cyperone-mediated suppression of ROS, cleaved-caspase-3, and Bax, as well as a-cyperone-induced Bcl-2 upregulation in H 2 O 2-treated SH-SY5Y cells. a-cyperone neuroprotection required Nrf2 activation. In conclusion, a-cyperone attenuated H 2 O 2-induced oxidative stress and apoptosis in SH-SY5Y cells via the activation of Nrf2, suggesting the potential of this compound in the prevention and treatment of PD.

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Section: A B C D Ementioning

confidence: 99%

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

Huang

Liu

et al. 2020

Front. Pharmacol.

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“…Besides, curcumin stimulates the migration of microglia to provide amyloid‐beta phagocytosis (Teter et al, 2019), leading to the alleviation of AD. To diminish the stress on neurons, curcumin induces heat shock protein 90 (HSP90), which is a key chaperone involved in the correct conformation of proteins, resulting in inhibition of apoptosis in dopaminergic neurons (Sang et al, 2018).…”

Section: Pharmacological Profile Of Curcuminmentioning

confidence: 99%

Versatile role of curcumin and its derivatives in lung cancer therapy

Ashrafizadeh

Najafi

Makvandi

et al. 2020

Journal Cellular Physiology

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Lung cancer is a main cause of death all over the world with a high incidence rate. Metastasis into neighboring and distant tissues as well as resistance of cancer cells to chemotherapy demand novel strategies in lung cancer therapy. Curcumin is a naturally occurring nutraceutical compound derived from Curcuma longa (turmeric) that has great pharmacological effects, such as anti‐inflammatory, neuroprotective, and antidiabetic. The excellent antitumor activity of curcumin has led to its extensive application in the treatment of various cancers. In the present review, we describe the antitumor activity of curcumin against lung cancer. Curcumin affects different molecular pathways such as vascular endothelial growth factors, nuclear factor‐κB (NF‐κB), mammalian target of rapamycin, PI3/Akt, microRNAs, and long noncoding RNAs in treatment of lung cancer. Curcumin also can induce autophagy, apoptosis, and cell cycle arrest to reduce the viability and proliferation of lung cancer cells. Notably, curcumin supplementation sensitizes cancer cells to chemotherapy and enhances chemotherapy‐mediated apoptosis. Curcumin can elevate the efficacy of radiotherapy in lung cancer therapy by targeting various signaling pathways, such as epidermal growth factor receptor and NF‐κB. Curcumin‐loaded nanocarriers enhance the bioavailability, cellular uptake, and antitumor activity of curcumin. The aforementioned effects are comprehensively discussed in the current review to further direct studies for applying curcumin in lung cancer therapy.

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“…Naringin can lead to the anti-inflammatory pathway and provide neuroprotection in PD [70]. In in vitro PD model of SH-SY5Y cells, curcumin imparts a role in neuroprotection against toxic insult via controlling the HSP-90 [71]. Curcumin was studied in Pheochromocytoma Cell Line 12 (PC12) cells against 1-methyl-4-phenylpyridinium (MPP + ) toxicity and fixation of mitochondrial membrane potential was found against enhancement of intracellular ROS which leads to the instigation of iNOS, and over-expression of Bcl-2 [144].…”

Section: Parkinson's Disease and Parkinsonismmentioning

confidence: 99%

Neuroprotection: Targeting Multiple Pathways by Naturally Occurring Phytochemicals

et al. 2020

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With the increase in the expectancy of the life span of humans, neurodegenerative diseases (NDs) have imposed a considerable burden on the family, society, and nation. In defiance of the breakthroughs in the knowledge of the pathogenesis and underlying mechanisms of various NDs, very little success has been achieved in developing effective therapies. This review draws a bead on the availability of the nutraceuticals to date for various NDs (Alzheimer’s disease, Parkinson’s disease, Amyotrophic lateral sclerosis, Huntington’s disease, vascular cognitive impairment, Prion disease, Spinocerebellar ataxia, Spinal muscular atrophy, Frontotemporal dementia, and Pick’s disease) focusing on their various mechanisms of action in various in vivo and in vitro models of NDs. This review is distinctive in its compilation to critically review preclinical and clinical studies of the maximum phytochemicals in amelioration and prevention of almost all kinds of neurodegenerative diseases and address their possible mechanism of action. PubMed, Embase, and Cochrane Library searches were used for preclinical studies, while ClinicalTrials.gov and PubMed were searched for clinical updates. The results from preclinical studies demonstrate the efficacious effects of the phytochemicals in various NDs while clinical reports showing mixed results with promise for phytochemical use as an adjunct to the conventional treatment in various NDs. These studies together suggest that phytochemicals can significantly act upon different mechanisms of disease such as oxidative stress, inflammation, apoptotic pathways, and gene regulation. However, further clinical studies are needed that should include the appropriate biomarkers of NDs and the effect of phytochemicals on them as well as targeting the appropriate population.

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Curcumin Protects an SH-SY5Y Cell Model of Parkinson’s Disease Against Toxic Injury by Regulating HSP90

Cited by 37 publications

References 24 publications

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

Versatile role of curcumin and its derivatives in lung cancer therapy

Neuroprotection: Targeting Multiple Pathways by Naturally Occurring Phytochemicals

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