Curcumin nanocochleates: Use of design of experiments, solid state characterization, in vitro apoptosis and cytotoxicity against breast cancer MCF-7 cells

Nadaf, Sameer J.; Killedar, Suresh G.

doi:10.1016/j.jddst.2018.06.026

Cited by 37 publications

(16 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BBD can study the effects and interactions between all factors and responses with the least number of experiments [29]. In our study, the four-factor, three-level experiment design was analysed with three responses to explore the best formulation.…”

Section: Resultsmentioning

confidence: 99%

Optimization and characterization of poly(lactic-co-glycolic acid) nanoparticles loaded with astaxanthin and evaluation of anti-photodamage effect in vitro

Liu

Yan

et al. 2019

R. Soc. open sci.

View full text Add to dashboard Cite

Astaxanthin is a xanthophyll carotenoid with high beneficial biological activities, such as antioxidant function and scavenging oxygen free radicals, but its application is limited because of poor water solubility and low bioavailability. Here, we prepared and optimized poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with astaxanthin using the emulsion solvent evaporation technique and investigated the anti-photodamage effect in HaCaT cells. The four-factor three-stage Box–Behnken design was used to optimize the nanoparticle formulation. The experimental determination of the optimal nanoparticle size was 154.4 ± 0.35 nm, the zeta potential was 22.07 ± 0.93 mV, encapsulation efficiency was 96.42 ± 0.73% and drug loading capacity was 7.19 ± 0.12%. The physico-chemical properties of the optimized nanoparticles were characterized by dynamic light scattering, scanning electron microscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, differential scanning calorimetry and thermo-gravimetric analyser. In vitro study exhibited the excellent cell viability and cellular uptake of optimized nanoparticles on HaCaT cells. The anti-photodamage studies (cytotoxicity assay, reactive oxygen species content and JC-1 assessment) demonstrated that the optimized nanoparticles were more effective and safer than pure astaxanthin in HaCaT cells. These results suggest that our PLGA-coated astaxanthin nanoparticles synthesis method was highly feasible and can be used in cosmetics or the treatment of skin diseases.

show abstract

Section: Resultsmentioning

confidence: 99%

Optimization and characterization of poly(lactic-co-glycolic acid) nanoparticles loaded with astaxanthin and evaluation of anti-photodamage effect in vitro

Liu

Yan

et al. 2019

R. Soc. open sci.

View full text Add to dashboard Cite

show abstract

“…Current treatments of cancer include chemotherapy, surgery, and radiation therapy, having hazardous adverse effects. From the ancient era, various types of cancer were cured and prevented by using natural and herbal drugs [2]. Epidermoid carcinoma, also known as skin cancer, is a more versatile and common cancer in human being.…”

Section: Introductionmentioning

confidence: 99%

Design and characterization of camptothecin gel for treatment of epidermoid carcinoma

Galatage¹,

Hebalkar²,

Gote³

et al. 2020

Futur J Pharm Sci

Self Cite

View full text Add to dashboard Cite

Background: The objective of present research work is to design and characterize camptothecin gel using Carbopol-934 for the treatment of epidermoid carcinoma. Optimized herbal gel formulations were evaluated for homogeneity and appearance, viscosity, extrudability, spreadability, drug content, drug release, pH, and in vitro skin cancer activity on A431 cell lines. Results: Mass and Infrared Spectra respectively conforms molecular weight and functional groups present in camptothecin. All the formulations F1 to F5 showed good homogeneity, pH from 6.68 to 6.90, spreadability in the range of 15.81-23.37 gm.cm/s, extrudability 85.51-90.45% w/w, drug content 89.12-96.64%, and in vitro diffusion 88.36-98.40%, respectively. The drug release study showed that all the formulations followed a diffusion-controlled, zero-order release mechanism. Anticancer activity results indicate that camptothecin gel induce cell death in A-439 cells having IC 50 48.03 μg and % apoptosis 54.67 ± 4.58. Conclusion: Topical delivery of camptothecin alleviates the side effects caused by systemic chemotherapy; hence, the developed herbal gel formulation can be effectively useful to deliver camptothecin in the treatment of epidermoid carcinoma on A-431 cells.

show abstract

“…Despite its effectiveness and curative potential, the use of curcumin as an anticancer agent is restricted due to poor aqueous solubility, poor tissue absorption, rapid systemic clearance, faster metabolism, rapid degradation at neutral-alkaline pH, and impaired tumor targeting. [6][7][8] To override these drawbacks, different nanoparticulate drug delivery systems such as liposomes, solid lipid nanoparticles, nanostructured lipid carriers, polymeric nanoparticles, micelles, and nanoemulsions, have been investigated. 9 On the same ground, we prepared the curcumin loaded nanocochleates (CU-NC) using solvent evaporation technique to avoid the problems associated with curcumin absorption.…”

Section: Introductionmentioning

confidence: 99%

“…Nanocochleates (NC) are stable rod-shaped phospholipid-cation precipitates and rolled cylindrical structures that can offer attractive characteristics, for example, improved efficacy, biocompatibility, and reduced side effects. 7 Notably, during the fabrication of diverse nanoparticulate systems, the encapsulation methods determine the percentage of encapsulants. In the case of the solvent evaporation technique, the amount of entrapped material is also subjective to partitioning between aqueous and organic phases.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of RP–HPLC Method for Estimation of Curcumin from Nanocochleates and Its Application in in–vivo Pharmacokinetic Study

Nadaf¹,

Killedar²

2020

ACSi

View full text Add to dashboard Cite

A reliable RP-HPLC analytical method with UV detection at 421 nm was developed and validated for the quantitative determination of curcumin from rat plasma after oral administration of curcumin loaded nanocochleates (CU-NC) to rats. The chromatographic separation was performed on HIQ SIL, C18 (250 mm × 4.6 mm) column using methanol and water (80:20 v/v) as mobile phase, at 1.0 mL/min flow rate. Validation parameters included linearity, accuracy, precision, and limit of quantitation and detection. Good linearity was obtained over the range of 2.5–100 μg/mL (R2 = 0.9979) of curcumin. The developed HPLC method was precise, with <2% relative standard deviation. Accuracy, stability, and robustness studies were also found to be acceptable. Bland-Altman plot showed an acceptable repeatability coefficient. The method was under statistical control, revealed by a control chart. After CU–NC administration, pharmacokinetic parameters i.e. Cmax, AUC0-∞, and AUMC0-∞, were observed to be 97.69 ± 10.84 μg/mL, 1402.77 ± 9.67 (μg/mL) ∙ h, and 35140.16 ± 14.67 (μg/mL) ∙ h2, respectively. This simple and precise method can be effectively implemented for routine analysis.

show abstract

Curcumin nanocochleates: Use of design of experiments, solid state characterization, in vitro apoptosis and cytotoxicity against breast cancer MCF-7 cells

Cited by 37 publications

References 49 publications

Optimization and characterization of poly(lactic-co-glycolic acid) nanoparticles loaded with astaxanthin and evaluation of anti-photodamage effect in vitro

Optimization and characterization of poly(lactic-co-glycolic acid) nanoparticles loaded with astaxanthin and evaluation of anti-photodamage effect in vitro

Design and characterization of camptothecin gel for treatment of epidermoid carcinoma

Development and Validation of RP–HPLC Method for Estimation of Curcumin from Nanocochleates and Its Application in in–vivo Pharmacokinetic Study

Contact Info

Product

Resources

About