Curcumin-loaded zein/pectin nanoparticles: Caco-2 cellular uptake and the effects on cell cycle arrest and apoptosis of human hepatoma cells (HepG2)

Co-Encapsulation of Tannic Acid and Resveratrol in Zein/Pectin Nanoparticles: Stability, Antioxidant Activity, and Bioaccessibility

“…The co-encapsulated pectin-coated nanoparticles were prepared according to our previous method [ 38 , 39 ], with some slight modifications: Briefly, 1.0 g of zein was solubilized in 50.0 mL of aqueous ethanol solution (85%, v / v ) with magnetic stirring at 500 rpm for 30 min (R05, IKA Inc., Staufen, Germany). Then weighed amounts of tannic acid (0.1 g) and resveratrol (0.12 g) were added into the zein solution, and it was continuously stirred in the dark for another 60 min.…”

“…Then, 4.0 mL of the tannic acid–resveratrol–zein solution was injected into 16 mL of double distilled water (adjusted to pH 4.0 with 0.1 M HCl) through a syringe and continually stirred at 900 rpm for 5 min. The ethanol in the prepared nanoparticle dispersion was evaporated with a rotary evaporator (RE-2000, Yarong Biochemical Instrument, Shanghai, China) at 40 °C, and an appropriate volume of pH 4.0 water was added until the final volume of the dispersion was 20 mL [ 38 ]. The resulting nanoparticle dispersion was then mixed with 20.0 mL of pectin solution (0.11% w / v , pH 4.0), with continuous stirring at 900 rpm for 10 min.…”

“…HepG2 cells were cultured in DMEM solution supplemented with 10% FBS and 1% penicillin-streptomycin solution. Cells were maintained at 37 °C under a humidified atmosphere with 5% CO 2 in an incubator [ 38 ]. Cells at 80–90% confluence were used for the treatments.…”

“…In previous studies, we developed pectin-coated zein nanoparticles that could be used as delivery system for bioactive agents [ 31 , 38 , 39 ]. We showed that the encapsulation of hydrophobic nutraceuticals (curcumin or resveratrol) in these delivery systems could increase their antioxidant activity, anti-inflammatory activity, bioaccessibility, and cellular uptake.…”

Effects of immersing treatment of curcumin and piperine combined with vacuum packaging on the quality of salmon (Salmo salar) during cold chain logistics

“…Therefore, it is necessary to study the effect of the extracts on different food products individually. Curcumin is a natural pigment mainly extracted from the rhizomes or tubers of various ginger plants, which has been widely used in the medical field because of its anti-inflammatory properties ( 11 ), promoting wound healing ( 12 ), regulating protein and metabolic pathways ( 13 ), inhibiting tumor growth and other pharmacological effects ( 14 ). Curcumin is also a food additive known for its antibacterial and antioxidant activity ( 15 ).…”