Curcumin-loaded lipid-core nanocapsules as a strategy to improve pharmacological efficacy of curcumin in glioma treatment

Zanotto-Filho, Alfeu; Coradini, Karine; Braganhol, Elizandra; Schröder, Rafael; Oliveira, Carlos Marcos Barcellos de; Simões-Pires, André; Battastini, Ana Maria Oliveira; Pohlmann, Adriana Raffin; Guterres, Sílvia Stanisçuaski; Forcelini, Cassiano Mateus; Beck, Ruy Carlos Ruver; Moreira, José Cláudio Fonseca

doi:10.1016/j.ejpb.2012.10.019

Cited by 138 publications

(106 citation statements)

References 47 publications

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“…[17][18][19] In addition, the enhanced antiproliferative response to combined treatment of two substances has been studied. A potential anticancer strategy with the combined use of raloxifene and trichostatin A (inhibitor to enhance the action of endocrine therapy in breast cancer cells) was observed on MCF-7 breast cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Controlled release of raloxifene by nanoencapsulation: effect on in vitro antiproliferative activity of human breast cancer cells

Fontana

Beckenkamp

Buffon

et al. 2014

IJN

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Raloxifene hydrochloride (RH) is considered to be an antiproliferative agent of mammary tissue. The aim of this study was to investigate the effect of the encapsulation of RH in polymeric nanocapsules with anionic or cationic surface on its release profile and antiproliferative activity. They were prepared by interfacial deposition of preformed polymer, followed by wide physicochemical characterization. The in vitro RH release was assessed by the dialysis membrane method and the data analyzed by mathematical modeling. The antiproliferative effect on MCF-7 cell viability was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay as well as by counting viable cells. They had high encapsulation efficiency, low polydispersity, and nanometric mean size. Nanocapsules prepared with Eudragit ® RS100 and Eudragit ® S100 presented positive and negative zeta potentials, respectively. Drug release studies demonstrated controlled release of RH from anionic nanocapsules, which could be explained due to a stronger interaction of the drug to these nanocapsules and the larger amount of entrapped drug. On the other hand, this control was not observed from cationic nanocapsules due to the larger amount of drug adsorbed onto their surface. MCF-7 cell viability studies and cell counting showed that RH-loaded Eudragit ® RS100 nanocapsules promote the best antiproliferative activity after 24 hours of treatment, whereas the best activity was observed for RH-loaded Eudragit ® S100 nanocapsules after 72 hours. Furthermore, the combined treatment of these formulations improved the antiproliferative effect during the entire treatment.

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Section: Introductionmentioning

confidence: 99%

Controlled release of raloxifene by nanoencapsulation: effect on in vitro antiproliferative activity of human breast cancer cells

Fontana

Beckenkamp

Buffon

et al. 2014

IJN

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“…6 These particles have an organogel core formed by a mixture of sorbitan monostearate and medium chain triglycerides, which increases drug encapsulation capacity by up to 40 times. 7 Recently, it has been described that the polymeric nanocapsules, prepared with poly(ε-caprolactone) (PCL) and polysorbate 80 (PS80), may improve the biological action 4,8 of drugs, increase the drug bioavailability, 9 and in case of antioxidant substances, their encapsulation has been shown to be able to improve their Dovepress Dovepress 5094 charão et al antioxidant properties. 4,[10][11][12] Moreover, PCL is being used for this purpose due to its biodegradability and biocompatibility in biological systems.…”

Section: Introductionmentioning

confidence: 99%

“…7 Recently, it has been described that the polymeric nanocapsules, prepared with poly(ε-caprolactone) (PCL) and polysorbate 80 (PS80), may improve the biological action 4,8 of drugs, increase the drug bioavailability, 9 and in case of antioxidant substances, their encapsulation has been shown to be able to improve their Dovepress Dovepress 5094 charão et al antioxidant properties. 4,[10][11][12] Moreover, PCL is being used for this purpose due to its biodegradability and biocompatibility in biological systems. 13 Furthermore, in 2006, Cruz et al 14 demonstrated the antiedematogenic effect of indomethacin ethyl ester-loaded LNC after oral administration.…”

Section: Introductionmentioning

confidence: 99%

“…1 Polymeric nanoparticles (NPs) obtained from biodegradable polymers are being studied as carriers for drug delivery. [2][3][4][5] Lipid-core nanocapsules (LNC) are a special type of particle due to the hybrid characteristics between polymeric and lipid NP. 6 These particles have an organogel core formed by a mixture of sorbitan monostearate and medium chain triglycerides, which increases drug encapsulation capacity by up to 40 times.…”

Section: Introductionmentioning

confidence: 99%

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Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Charão

Souto

Brucker

et al. 2015

IJN

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Caenorhabditis elegans is an alternative in vivo model that is being successfully used to assess the pharmacological and toxic effects of drugs. The exponential growth of nanotechnology requires the use of alternative in vivo models to assess the toxic effects of theses nanomaterials. The use of polymeric nanocapsules has shown promising results for drug delivery. Moreover, these formulations have not been used in cases of intoxication, such as in treatment of paraquat (PQ) poisoning. Thus, the use of drugs with properties improved by nanotechnology is a promising approach to overcome the toxic effects of PQ. This research aimed to evaluate the absorption of rhodamine B-labeled melatonin (Mel)-loaded lipid-core nanocapsules (LNC) by C. elegans , the application of this model in nanotoxicology, and the protection of Mel-LNC against PQ damage. The formulations were prepared by self-assembly and characterized by particle sizing, zeta potential, drug content, and encapsulation efficiency. The results demonstrated that the formulations had narrow size distributions. Rhodamine B-labeled Mel-LNC were orally absorbed and distributed in the worms. The toxicity assessment of LNC showed a lethal dose 50% near the highest dose tested, indicating low toxicity of the nanocapsules. Moreover, pretreatment with Mel-LNC significantly increased the survival rate, reduced the reactive oxygen species, and maintained the development in C. elegans exposed to PQ compared to those worms that were either untreated or pretreated with free Mel. These results demonstrated for the first time the uptake and distribution of Mel-LNC by a nematode, and indicate that while LNC is not toxic, Mel-LNC prevents the effects of PQ poisoning. Thus, C. elegans may be an interesting alternative model to test the nanocapsules toxicity and efficacy.

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“…This was demonstrated for a similar LNC formulation by uptake experiments in the presence of endocytosis inhibitors, with results showing almost complete LNC drug content uptake inhibition by glioma cells. 36 Also, the present results suggest that the molecular mechanisms associated with apoptosis in the MDA-MB-231 cell line are more sensitive to the nanoformulation evaluated in this study. In this cell line, we observed apoptosis induction by LNCs, without the presence of the drug.…”

Section: A3mentioning

confidence: 50%

Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line

Yurgel

Oliveira

Begnini

et al. 2014

IJN

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Breast cancer is the most frequent cancer affecting women. Methotrexate (MTX) is an antimetabolic drug that remains important in the treatment of breast cancer. Its efficacy is compromised by resistance in cancer cells that occurs through a variety of mechanisms. This study evaluated apoptotic cell death and cell cycle arrest induced by an MTX derivative (MTX diethyl ester [MTX(OEt) 2 ]) and MTX(OEt) 2 -loaded lipid-core nanocapsules in two MTX-resistant breast adenocarcinoma cell lines, MCF-7 and MDA-MB-231. The formulations prepared presented adequate granulometric profile. The treatment responses were evaluated through flow cytometry. Relying on the mechanism of resistance, we observed different responses between cell lines. For MCF-7 cells, MTX(OEt) 2 solution and MTX(OEt) 2 -loaded lipid-core nanocapsules presented significantly higher apoptotic rates than untreated cells and cells incubated with unloaded lipid-core nanocapsules. For MDA-MB-231 cells, MTX(OEt) 2 -loaded lipid-core nanocapsules were significantly more efficient in inducing apoptosis than the solution of the free drug. S-phase cell cycle arrest was induced only by MTX(OEt) 2 solution. The drug nanoencapsulation improved apoptosis induction for the cell line that presents MTX resistance by lack of transport receptors.

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Curcumin-loaded lipid-core nanocapsules as a strategy to improve pharmacological efficacy of curcumin in glioma treatment

Cited by 138 publications

References 47 publications

Controlled release of raloxifene by nanoencapsulation: effect on in vitro antiproliferative activity of human breast cancer cells

Controlled release of raloxifene by nanoencapsulation: effect on in vitro antiproliferative activity of human breast cancer cells

Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line

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