Curcumin Loaded Chitosan-Protamine Nanoparticles Revealed Antitumor Activity Via Suppression of NF-κB, Proinflammatory Cytokines and Bcl-2 Gene Expression in the Breast Cancer Cells

Abd-Elhakeem, Mohamed A.; Mongy, Sara; Hassan, Basant; Tantawi, Omnia I.; Badawy, Ingy

doi:10.1016/j.xphs.2021.06.004

Cited by 34 publications

(12 citation statements)

References 40 publications

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“…Herein, cytotoxic studies of free and loaded GM were performed on HSF human fibroblast cells. In agreement with the previous studies [ 28 , 29 ], the CSNPs showed no cytotoxic effect on HSF cells after 72 h of incubation. This finding confirmed the nontoxicity and biocompatibility of the used carrier.…”

Section: Discussionsupporting

confidence: 93%

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Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity

et al. 2022

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Nano-drug delivery is a promising tactic to enhance the activity and minimize the cytotoxicity of antimicrobial drugs. In the current study, chitosan nanoparticles (CSNPs) were used as a carrier for the delivery of gentamicin sulfate (GM) and ascorbic acid (AA). The particles were synthesized by ionotropic gelation method and characterized by FT-IR, Zeta potential, and transmission electron microscope imaging. The obtained particles were evaluated for their in vitro antimicrobial activity and cytotoxicity. The prepared particles (GM–AA–CSNPs) under the optimal condition of 4:1:1 of chitosan to drug ratio showed encapsulation efficiency and loading capacities of 89% and 22%, respectively. Regarding biological activities, GM–AA–CSNPs showed a lower minimum inhibitory concentration (MIC) than free gentamicin sulfate and GMCSNPs mixture without presenting cytotoxicity against normal cells (HSF). Moreover, the GM–AA–CSNPs did not exhibit hemolytic activity. These results highlight that the GM–AA–CSNPs are confirmed as a hopeful formula for future investigations on the development of antimicrobial preparations.

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Section: Discussionsupporting

confidence: 93%

“…The therapeutic effectiveness of the nano-formulated drug is related to the high EE% and LC% and a prolonged release profile [ 28 , 29 ]. Herein, Formula 3 (CS: drug ratio of 4:1:1) showed EE% values higher than 80% for both GM and AA in GM–AA–CSNPs, indicating the high efficiency of the carrier for loading both kinds of drugs.…”

Section: Discussionmentioning

confidence: 99%

Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity

et al. 2022

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“…Numerous studies have shown that curcumin has various physiological activities such as inhibiting tumor cell proliferation and promoting apoptosis [ 14 ]. Curcumin promotes tumor cell apoptosis by inhibiting NF-κB in breast cancer cell line MCF-7, pancreatic stellate cells, and liver cancer stem cells [ 15 , 16 , 17 ]. Curcumin may increase the activity of other chemotherapeutic agents such as paclitaxel.…”

Section: Natural Compounds That Synergistically Enhance the Effects O...mentioning

confidence: 99%

Exploration of the Use of Natural Compounds in Combination with Chemotherapy Drugs for Tumor Treatment

et al. 2023

Molecules

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Currently, chemotherapy is the main treatment for tumors, but there are still problems such as unsatisfactory chemotherapy results, susceptibility to drug resistance, and serious adverse effects. Natural compounds have numerous pharmacological activities which are important sources of drug discovery for tumor treatment. The combination of chemotherapeutic drugs and natural compounds is gradually becoming an important strategy and development direction for tumor treatment. In this paper, we described the role of natural compounds in combination with chemotherapeutic drugs in synergizing, reducing drug resistance, mitigating adverse effects and related mechanisms, and providing new insights for future oncology research.

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“…Chitosan nanoparticles loaded with carnosic acid (CA-CH-NPs) were prepared by the ionic gelation method [26]. Initially, 100 mg of chitosan was dissolved in 2% aqueous acetic acid (20 mL), and then 10 mg of carnosic acid was added.…”

Section: Synthesis Of Ca-loaded Chitosan Nanoparticlesmentioning

confidence: 99%

Carnosic Acid Encapsulated in Albumin Nanoparticles Induces Apoptosis in Breast and Colorectal Cancer Cells

et al. 2022

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Carnosic acid (CA) is a natural phenolic compound with several biomedical actions. This work was performed to study the use of CA-loaded polymeric nanoparticles to improve the antitumor activity of breast cancer cells (MCF-7) and colon cancer cells (Caco-2). CA was encapsulated in bovine serum albumin (BSA), chitosan (CH), and cellulose (CL) nanoparticles. The CA-loaded BSA nanoparticles (CA-BSA-NPs) revealed the most promising formula as it showed good loading capacity and the best release rate profile as the drug reached 80% after 10 h. The physicochemical characterization of the CA-BSA-NPs and empty carrier (BSA-NPs) was performed by the particle size distribution analysis, transmission electron microscopy (TEM), and zeta potential. The antitumor activity of the CA-BSA-NPs was evaluated by measuring cell viability, apoptosis rate, and gene expression of GCLC, COX-2, and BCL-2 in MCF-7 and Caco-2. The cytotoxicity assay (MTT) showed elevated antitumor activity of CA-BSA-NPs against MCF-7 and Caco-2 compared to free CA and BSA-NPs. Moreover, apoptosis test data showed an arrest of the Caco-2 cells at G2/M (10.84%) and the MCF-7 cells at G2/M (4.73%) in the CA-BSA-NPs treatment. RT-PCR-based gene expression analysis showed an upregulation of the GCLC gene and downregulation of the BCL-2 and COX-2 genes in cells treated with CA-BSA-NPs compared to untreated cells. In conclusion, CA-BSA-NPs has been introduced as a promising formula for treating breast and colorectal cancer.

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Curcumin Loaded Chitosan-Protamine Nanoparticles Revealed Antitumor Activity Via Suppression of NF-κB, Proinflammatory Cytokines and Bcl-2 Gene Expression in the Breast Cancer Cells

Cited by 34 publications

References 40 publications

Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity

Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity

Exploration of the Use of Natural Compounds in Combination with Chemotherapy Drugs for Tumor Treatment

Carnosic Acid Encapsulated in Albumin Nanoparticles Induces Apoptosis in Breast and Colorectal Cancer Cells

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