Curcumin inhibits UVB‐induced matrix metalloproteinase‐1/3 expression by suppressing the MAPK‐p38/JNK pathways in human dermal fibroblasts

Hwang, Bomi; Noh, Eun‐Mi; Kim, Jong‐Suk; Kim, Jeong-Mi; You, Yong‐Ouk; Hwang, Jin‐Ki; Kwon, Kang‐Beom; Lee, Young‐Rae

doi:10.1111/exd.12137

Cited by 51 publications

(40 citation statements)

References 16 publications

(21 reference statements)

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“…PPAR-γ ligand rosiglitazone (Sigma-Aldrich, USA) was dissolved in DMSO and added to cultured cells to the final concentrations of 5, 10, 20, and 40 μM as described previously (Hogan et al, 2011). Curcumin (Sigma-Aldrich, USA) was prepared in the same manner and added to cultured cells to the final concentrations of 5, 25, and 50 μM as described previously (Hwang et al, 2013). Recombinant mouse TGF-β2 (R&D Systems, USA) was used at a final concentration of 10 ng/ml as described previously (Ma et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

Curcumin Inhibits Transforming Growth Factor β Induced Differentiation of Mouse Lung Fibroblasts to Myofibroblasts

et al. 2016

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Transforming growth factor β (TGF-β) induced differentiation of lung fibroblasts to myofibroblasts is a key event in the pathogenesis of pulmonary fibrosis. This study aimed to evaluate the effect of curcumin on TGF-β induced differentiation of lung fibroblasts to myofibroblasts and explore the underlying mechanism. Mouse lung fibroblasts were cultured and treated with TGF-β2 and curcumin or rosiglitazone. Cell vitality was examined by MTT assay. The secretion of collagen-1 was assessed by ELISA. α smooth muscle actin (α-SMA) was visualized by immunofluorescence technique. The expression of peroxisome proliferator activated receptor γ (PPAR-γ) and platelet derived growth factor R β (PDGFR-β) was detected by PCR and Western blot analysis. We found that curcumin and rosiglitazone inhibited the proliferation and TGF-β induced differentiation of mouse lung fibroblasts. In addition, curcumin and rosiglitazone inhibited collagen-1 secretion and α-SMA expression in mouse lung fibroblasts. Furthermore, curcumin and rosiglitazone upregulated PPAR-γ and downregulated PDGFR-β expression in mouse lung fibroblasts. In conclusion, our study reveals novel mechanism by which curcumin inhibits TGF-β2 driven differentiation of lung fibroblasts to myofibroblasts. Curcumin could potentially be used for effective treatment of pulmonary fibrosis.

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Section: Methodsmentioning

confidence: 99%

Curcumin Inhibits Transforming Growth Factor β Induced Differentiation of Mouse Lung Fibroblasts to Myofibroblasts

et al. 2016

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“…These bioactive compounds continue to gain popularity based on their several advantages, which include often having fewer side effects, showing better patient tolerance, and being relatively less expensive and acceptable due to a long history of use compared to synthetic ingredients [5]. In the search for effective drug leads from herbs used against skin abnormalities, bioactive compounds such as aloin [6], curcumin [7], resveratrol [8], gallic acid [9], quercetin, genistein and catechins [10] have been found.…”

Section: Introductionmentioning

confidence: 99%

The Potential of Triterpenoids from Loquat Leaves (Eriobotrya japonica) for Prevention and Treatment of Skin Disorder

Tao

Tamrakar

2017

IJMS

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The leaves of loquat (Eriobotrya japonica) possess high medicinal value and have been used as traditional medicines. However, there are no evidence-based studies on the skin-care effects of E. japonica leaves. To explore new biological activities of E. japonica leaves against skin disorder and to gain a better understanding of the chemical components associated with bioactivities, we evaluated 18 triterpenoids from E. japonica leaves on anti-melanogenesis, anti-acne, anti-allergy and anti-aging activities. Our results revealed that eight compounds showed anti-melanogenesis activity, of which ursolic acid (1) and maslinic acid (7) were the most potent with the similar selective index to that of arbutin. Structure–activity relationship and possible mechanism of active compounds were proposed. Twelve compounds exhibited anti-acne effect; ursolic acid (1), maslinic acid (7), corosolic acid (8) and euscaphic acid (12) showed highest activities against P. acnes. Four compounds displayed anti-allergy and anti-inflammatory activity; 3-epicorosolic acid (9) and euscaphic acid (12) showed marked activity against β-hexosaminidase release. Finally, ursolic acid (1), pomolic acid (10), colosolic acid (8) and its methylated derivative (6) exhibited the highest anti-aging activity by stimulating collagen and hyaluronic acid (HA) production. Our findings provide valuable evidence that E. japonica leaves have potential applications as ingredients of function foods or cosmetics for health benefits and a number of triterpenoids may play an important role in these bioactivities.

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“…In a study of diabetic rats, MMP9 gene expression was found to be activated by the loss of H3K9me2 and the increase of H3K9ac at the NFκB binding site in the MMP9 promoter (6). The role of histone modification changes in regulating the response of dermal fibroblasts to UVR is not understood as well as the regulation of MMPs by UVR-induced signal transduction pathways and transcription factors (7). Epigenetic responses to UVR may contribute to the up regulation of MMPs, matrix remodeling, and photoaging.…”

Section: Introductionmentioning

confidence: 99%

Solar‐simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts

Gesumaria

Matsui

Kluz

et al. 2015

Experimental Dermatology

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Molecular signaling pathways delineating the induction of matrix metalloproteinases (MMPs) by ultraviolet radiation (UVR) are currently well-defined; however, the effects of UVR on epigenetic mechanisms of MMP induction are not as well understood. In this study, we examined solar simulated UVR (ssUVR)-induced gene expression changes and alterations to histone methylation in the promoters of MMP1 and MMP3 in primary human dermal fibroblasts (HDF). Gene expression changes, including the increased expression of MMP1 and MMP3, were observed using Affymetrix GeneChip arrays and confirmed by qRT-PCR. Using ChIP-PCR, we showed for the first time that in HDF irradiated with 12 J/cm2 ssUVR, the H3K4me3 transcriptional activating mark increased and the H3K9me2 transcriptional silencing mark decreased in abundance in promoters, correlating with the observed elevation of MMP1 and MMP3 mRNA levels following ssUVR exposure. Changes in mRNA levels due to a single exposure were transient and decreased 5 days after exposure.

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Curcumin inhibits UVB‐induced matrix metalloproteinase‐1/3 expression by suppressing the MAPK‐p38/JNK pathways in human dermal fibroblasts

Cited by 51 publications

References 16 publications

Curcumin Inhibits Transforming Growth Factor β Induced Differentiation of Mouse Lung Fibroblasts to Myofibroblasts

Curcumin Inhibits Transforming Growth Factor β Induced Differentiation of Mouse Lung Fibroblasts to Myofibroblasts

The Potential of Triterpenoids from Loquat Leaves (Eriobotrya japonica) for Prevention and Treatment of Skin Disorder

Solar‐simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts

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