Curcumin inhibits B[a]PDE‐induced procarcinogenic signals in lung cancer cells, and curbs B[a]P‐induced mutagenesis and lung carcinogenesis

Abstract

Benzo[a]pyrene is a procarcinogen present in environment and cigarette smoke, which could be bio-transformed in vivo to B[a]PDE, a potent carcinogen known to form DNA adducts and induce mutations. We observed that curcumin, a known chemopreventive, could significantly inhibit the survival of lung cancer cells exposed to B[a]PDE. It also downregulates B[a]PDE-induced nuclear translocation of NF-κB as assessed by Electrophoretic Mobility Shift Assay (EMSA) and NF-κB-dependent reporter gene assay. Ames assay demonstrated its ability to revert the mutagenic property of benzo[a]pyrene. These observations prompted us to evaluate the efficacy of curcumin in preventing B[a]P-induced lung carcinogenesis in vivo and to explore the molecular mechanism associated with it. The average number of tumor nodules present in the lungs of the Swiss albino mice, which received benzo[a]pyrene, was significantly high compared to that received curcumin as 2% diet along with B[a]P. Curcumin treatment significantly reverted histopathological deviations in the lung tissues due to benzo[a]pyrene ingestion. Moreover, curcumin diet reduced benzo[a]pyrene-induced activation of NF-κB and MAPK signaling and Cox-2 transcription in lung tissues of mice. Taken together, this study illustrates multifaceted efficacy of curcumin in preventing lung cancer.

Chitosan Encapsulation Enhances the Bioavailability and Tissue Retention of Curcumin and Improves its Efficacy in Preventing B[a]P-induced Lung Carcinogenesis

“…Curcumin is a chemopreventive agent, which possesses multifaceted potentials to prevent cancer progression (15). There are several studies demonstrating the efficacy of curcumin in preventing cancer progression induced by protumorigenic agents and environmental mutagens (16,17). Several reports, including those from our laboratory, have shown the chemopreventive efficacy of curcumin against lung cancer (17,18).…”

“…There are several studies demonstrating the efficacy of curcumin in preventing cancer progression induced by protumorigenic agents and environmental mutagens (16,17). Several reports, including those from our laboratory, have shown the chemopreventive efficacy of curcumin against lung cancer (17,18). Although there are ample reports highlighting the anticancer potential of curcumin, the major hindrance in employing it for cancer therapy and prevention, stems from its poor aqueous solubility and bioavailability (19).…”

Deoxyelephantopin induces apoptosis in HepG2 cells via oxidative stress, NF‐κB inhibition and mitochondrial dysfunction

“…However, in most cancers including hepatocellular carcinoma, it is constitutively activated . Activation of NF‐κB is most commonly mediated by IκB kinase (IKK) complex which inhibits IκBα by decreasing phosphorylation . Upon phosphorylation of IκBα, NF‐κB dimmers get free and translocated into nucleus where it activates genes that are crucial for survival, antiapoptosis, drug resistance, angiogenesis, invasion and metastasis .…”

“…Activation of NF‐κB is most commonly mediated by IκB kinase (IKK) complex which inhibits IκBα by decreasing phosphorylation . Upon phosphorylation of IκBα, NF‐κB dimmers get free and translocated into nucleus where it activates genes that are crucial for survival, antiapoptosis, drug resistance, angiogenesis, invasion and metastasis . Recent research has shown that NF‐κB is activated in response to prevailing chemotherapeutic drugs which plays a vital role in poor prognosis of various cancers including liver cancer .…”

Health risk in transport workers. Part II. Dietary compounds as modulators of occupational exposure to chemicals

“…Apart from the studies aiming at analyzing the influence of individual products with a specific content on the cascade of changes induced by BaP, there are many papers describing the influence of isolated single compounds, such as vitamin C, β-carotenoids, retinol, retinoic acid, phenethyl isothiocyanate, luteolin, chrysin, naringenin, quercetin, damnacanthal, proanthocyanidine, resveratrol, berberine, catechin, anthocyanins, apigenin or kaempferol. The mechanisms of action of the selected phytochemicals and the above-mentioned dietary compounds are presented in Table 1 [52][53][54][55][56][57][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73].…”