Curcumin inhibits human retinal pigment epithelial cell proliferation

Sun, Yun; You, Zhipeng

doi:10.3892/ijmm.2014.1861

Cited by 15 publications

(16 citation statements)

References 43 publications

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“…Curcumin is a monomer component from traditional Chinese medicine that exhibits anti-proliferation, anti-tumor, and anti-inflammatory effects, among others [8,9]. Previous studies show that curcumin can inhibit RPE cell proliferation in vitro [10,11]; however, there are no reports on whether this inhibition is achieved by downregulating EGF expression. In the current study, different concentrations of curcumin were used to treat RPE cells cultured in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, curcumin has many benefits, such as a high level of safety, low toxicity, a wide range of treatment uses, and low cost. Curcumin also inhibits RPE cell proliferation [10][11][12], although it is unknown whether it plays an important role in the prevention of PVR formation. However, curcumin has been reported to regulate the biological functions of EGF, which plays important roles in the development of PVR.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Curcumin on Epidermal Growth Factor in Proliferative Vitreoretinopathy

Ren

Zhao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Proliferative vitreoretinopathy (PVR) is a common refractory eye disease that causes blindness and occurs after retinal detachment or retinal reattachment. Epidermal growth factor (EGF) has been shown to play an important role in the migration and proliferation of retinal pigment epithelium (RPE) cells, which promote PVR. Curcumin inhibits RPE cell proliferation, but it is not known whether it participates in the formation of PVR. Curcumin regulates the biological functions of EGF, which plays important roles in the development of PVR. This study aimed to evaluate the effect of curcumin on the regulation of EGF in PVR. Methods: Rabbit RPE cells were cultured, and EGF expression was detected by immunocytochemistry. MTT assay was conducted to determine cell proliferation induced by different concentrations of EGF. Immunocytochemical staining was used to detect EGF expression after treatment with curcumin at varying concentrations. Real-time PCR (RT-PCR) and western blot analysis were used to detect the concentrations of EGF mRNA and protein after treatment with curcumin. After RPE cells and curcumin were injected into experimental rabbit eyes, the cornea, aqueous humor, lens, and vitreous opacity were observed and recorded simultaneously by indirect ophthalmoscopy, fundus color photography, and B-ultrasonography. The vitreous body was extracted, and the EGF content in the vitreous humor was measured by enzyme-linked immunosorbent assay (ELISA). Results: At each time point (24, 48, and 72 h), cell proliferation gradually increased with increasing EGF concentrations (0, 3, 6, and 9 ng/mL) in a dose-dependent manner. Cell proliferation between EGF concentrations of 9 and 12 ng/mL were no different, which suggested that 9 ng/mL EGF was the best concentration to use to stimulate RPE cell proliferation in vitro. Under all EGF concentrations (0, 3, 6, 9, and 12 ng/mL), RPE cell proliferation increased with time (from 24 to 72 h), suggesting a time–effect relationship. Curcumin downregulated EGF expression in RPE cells, which also indicated time–effect and dose–effect relationships. The best curcumin concentration for the inhibition of EGF expression was 15 µg/mL. RT-PCR and western blot analyses indicated that the EGF mRNA and expression of the protein in RPE cells treated with curcumin significantly decreased with time. Ocular examinations revealed that the vitreous opacity was lower and the proliferative membrane was thinner in the curcumin group compared with the control group. The PVR grade and the incidence of retinal detachment were significantly lower in the experimental group than in the control group. ELISA results showed that the EGF content in vitreous humor was higher in the control group than in the curcumin group. The curcumin and control groups were significantly different at each time point. Conclusion: Curcumin inhibited RPE cell proliferation by downregulating EGF and thus effectively inhibited the initiation and development of PVR.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Curcumin on Epidermal Growth Factor in Proliferative Vitreoretinopathy

Ren

Zhao

et al. 2018

Cell Physiol Biochem

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“…NORFLO is a curcumin-phosphatidylcholine complex (iphytoone) with recently described anti-inflammatory properties. 7 –10 Although the mechanism is yet to be fully elucidated, several studies reported the beneficial effects of curcumin in many relapsing ophthalmic diseases sustained by long-standing inflammation such as central serous chorioretinopathy, diabetic retinopathy, dry eye, and uveitis of different etiologies. 11 –13,25 –29 In the present series, we reported an improvement of chronic 1+ anterior chamber flare after a 1 year course of oral therapy with NORFLO tablets.…”

Section: Discussionmentioning

confidence: 99%

Oral phospholipidic curcumin in juvenile idiopathic arthritis-associated uveitis

Miserocchi

Giuffrè

Cicinelli

et al. 2019

European Journal of Ophthalmology

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Purpose: To evaluate the efficacy and the safety of curcumin-phosphatidylcholine complex in children affected by juvenile idiopathic arthritis–associated uveitis as an adjunctive treatment to chronic systemic immunosuppressive therapy. Methods: In this retrospective, longitudinal study, we treated patients affected by juvenile idiopathic arthritis–associated uveitis with residual low-grade inflammatory activity in the anterior chamber with one tablet of curcumin-phosphatidylcholine complex per day, over a year. Low-grade inflammatory activity was characterized by flare 1+ at slit-lamp examination and 10–50 photon counts per ms) at the FC500 laser flare meter. Inactivity of uveitis was defined as complete disappearance of flare at the slit-lamp examination and values <10 ph/ms at laser flare meter. Conversely, recurrence of the uveitis was defined as a one-step increase from baseline in anterior chamber cells levels or laser flare meter measurements >50 ph/ms. Results: A total of 22 out of 27 patients (81%) achieved inactivity at the end of the study. Five patients (19%) did not show a significant reduction in anterior chamber flare, remaining stable throughout the follow-up. Only three episodes of flare-ups in three different patients were recorded. Overall, the treatment was well tolerated by all patients and no ocular discomfort, ocular side effects, or allergic reactions were registered. Conclusion: Adjunctive therapy with curcumin in patients affected by juvenile idiopathic arthritis–associated uveitis improves mild chronic anterior chamber flare and presents a good safety profile. Despite being mild, anterior chamber inflammation should be minimized to avoid the development of sight-threatening complications in these patients.

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“…1b). Curcumin (15 μM) treatment of RPE cells was previously shown to arrest cell cycle progression with arrest in the G1 phase [12]. Cell cycle progression of ARPE-19 cells exposed to 0-50 μM nano-curcumin did not show significant changes in cell proliferation (Fig.…”

Section: Nano-curcumin Is Not Cytotoxic For Arpe-19 Cells Unlike Curmentioning

confidence: 94%

Modulation of the Proteasome Pathway by Nano-Curcumin and Curcumin in Retinal Pigment Epithelial Cells

Carvalho¹,

Verwoert²,

Vogels³

et al. 2017

Ophthalmic Res

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Introduction: Curcumin has multiple biological effects including the modulation of protein homeostasis by the ubiquitin-proteasome system. The purpose of this study was to assess the in vitro cytotoxic and oxidative effects of nano-curcumin and standard curcumin and characterize their effects on proteasome regulation in retinal pigment epithelial (RPE) cells. Methods: Viability, cell cycle progression, and reactive oxygen species (ROS) production were determined after treatment with nano-curcumin or curcumin. Subsequently, the effects of nano-curcumin and curcumin on proteasome activity and the gene and protein expression of proteasome subunits PA28α, α₇, β₅, and β_5i were assessed. Results: Nano-curcumin (5-100 μM) did not show significant cytotoxicity or anti-oxidative effects against H₂O₂-induced oxidative stress, whereas curcumin (≥10 μM) was cytotoxic and a potent inducer of ROS production. Both nano-curcumin and curcumin induced changes in proteasome-mediated proteolytic activity characterized by increased activity of the proteasome subunits β₂ and β_5i/β₁ and reduced activity of β₅/β_1i. Likewise, nano-curcumin and curcumin affected mRNA and protein levels of household and immunoproteasome subunits. Conclusions: Nano-curcumin is less toxic to RPE cells and less prone to induce ROS production than curcumin. Both nano-curcumin and curcumin increase proteasome-mediated proteolytic activity. These results suggest that nano-curcumin may be regarded as a proteasome-modulating agent of limited cytotoxicity for RPE cells.

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Curcumin inhibits human retinal pigment epithelial cell proliferation

Cited by 15 publications

References 43 publications

Effects of Curcumin on Epidermal Growth Factor in Proliferative Vitreoretinopathy

Effects of Curcumin on Epidermal Growth Factor in Proliferative Vitreoretinopathy

Oral phospholipidic curcumin in juvenile idiopathic arthritis-associated uveitis

Modulation of the Proteasome Pathway by Nano-Curcumin and Curcumin in Retinal Pigment Epithelial Cells

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