Curcumin inhibits adipocyte differentiation through modulation of mitotic clonal expansion

Kim, Choon Young; Le, Thuc T.; Chen, Chihyu; Cheng, Ji‐Xin; Kim, Kee‐Hong

doi:10.1016/j.jnutbio.2010.08.003

Cited by 137 publications

(103 citation statements)

References 43 publications

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“…Our results are similar to the report of study on mouse G8 myoblasts [7]. Ectopic expressions of PPARγ and C/EBPα in fibroblastic cell lines are induced transdifferentiation into adipocytes [6,9]. Some studies show that expression of either PPARγ or C/EBPα is sufficient to induce transdifferentiation in goat primary myoblast cells and C2C12 mouse myoblast cell line [6,19].…”

Section: Resultssupporting

confidence: 89%

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Effects of Ectopic Expression of Transcription Factors on Adipogenic Transdifferentiation in Bovine Myoblasts

Moon¹

2012

Journal of Life Science

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The present study was conducted to investigate whether myoblasts can be transdifferentiated into adipocytes by ectopic expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein-α (C/EBPα), sterol regulatory element binding protein-1c (SREBP1c), and Krueppel-like factor 5 (KLF5), in primary bovine satellite cells. Transcription factors were transiently transfected into primary bovine myoblasts, and the cells were cultured with adipogenic differentiation medium for 2 days and then cultured on growth medium for an additional 8 days. Ectopic expression of PPARγ or C/EBPα alone was insufficient to induce adipogenesis in myoblasts. However, overexpression of both PPARγ and C/EBPα in myoblasts was able to induce adipogenic transdifferentiation as indicated by the appearance of mature adipocytes, the induction of adipogenic gene expressions, and the suppression of myogenic gene expressions. In addition, KLF5 and PPARγ co-transfected bovine myoblasts were converted to adipocytes but not in cells transfected with only KLF5 expression vector. Overexpression of SREBP1c alone was sufficient to induce transdifferentiation from myoblasts into adipocytes. These results demonstrate that primary bovine satellite cells can be transdifferentiated into adipocytes either by single ectopic expression or combined expression of adipogenic transcription factors in a culture system.

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Section: Resultssupporting

confidence: 89%

“…Members of the KLF family of transcription factors are important regulators of development, cellular differentiation and growth in tumor development [9]. Recent studies also suggest a potential role for KLFs in adipogenesis [9,14].…”

Section: Effects Of Klf5 On Adipogenic Transdifferentiation In Myoblastsmentioning

confidence: 99%

Effects of Ectopic Expression of Transcription Factors on Adipogenic Transdifferentiation in Bovine Myoblasts

Moon¹

2012

Journal of Life Science

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“…Many natural compounds have been reported to inhibit adipogenesis by affecting MCE. Sulforaphane arrests cells in the G0/G1 phase ( 41 ); widdrol causes cell cycle arrest in the G1/S-phase transition ( 45 ); genistein, piceatannol, and curcumin arrest cells in the S/G2/M phase ( 46 ); and garcinol and pterostilbene cause G2/M-phase arrest ( 47 ). Rohitukine decreases CDK4, CDK6, CDK2, cyclin-D, and cyclin-E and stabilizes P27 levels ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The LA compound sinapinic acid had not been reported yet for antidyslipidemic or hypolipidemic activity. Besides this, well known antiadipogenic compounds such as curcumin ( 46 ), cordycepin ( 39 ), berberine ( 53 ), piceatannol ( 43 ) and daidzein ( 54 ) also showed antidyslipidemic and hypolipidemic activity in a hamster model ( 51,(55)(56)(57)(58). Most of the studies were conducted using the Syrian golden hamster because it is a very sensitive model system for changes in lipid profi le when fed with HFD.…”

Section: Discussionmentioning

confidence: 99%

Rohitukine inhibits in vitro adipogenesis arresting mitotic clonal expansion and improves dyslipidemia in vivo

Varshney

Shankar

Beg

et al. 2014

Journal of Lipid Research

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“…Different in vitro assays ( Table 1) have demonstrated that curcumin inhibits adipocyte differentiation [54,55] and decreases lipid accumulation by reducing the expression of fatty acid synthase (FAS), as well as by down-regulating the PPAR-γ and CD36 mRNA levels during adipocyte differentiation [54]. In C57/BL mice ( Table 2), curcumin reduced body weight gain and adiposity, decreased serum cholesterol and caused suppression of angiogenesis in adipose tissue [56].…”

Section: Curcuminmentioning

confidence: 99%

Studies on Mechanistic Role of Natural Bioactive Compounds in the Management of Obesity An Overview

Alves¹

2012

TONUTRAJ

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Abstract:Obesity is recognised as a condition of low-grade chronic inflammation resulting from macrophage infiltration of adipose tissue and activation of inflammatory pathways by oxidative stress mechanisms that lead to the development of insulin resistance. Various natural bioactive compounds (NBCs) with anti-inflammatory and anti-oxidant effects may improve adipocyte dysfunction associated with metabolic syndrome. The present review focuses on the effects of phenolic compounds, n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) and lipoic acid (LA) on the pathophysiological mechanisms of obesity. In this review, a total of 120 studies were included, and data thus obtained reflect beneficial physiological effects of n-3 LC-PUFA, LA and different phenolic compounds, including kaempferol, luteolin, apigenin, quercetin, resveratrol, curcumin, catechins, phenolic acids, in the prevention and/or attenuation of metabolic disturbances associated with obesity. Additionally, information from clinical studies provides new insights for defining the doseresponse relationship of dietary compounds, necessary time of exposure and potential side effects of these NBCs in the treatment of obesity and indicates further study is needed to verify these relationships.

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Curcumin inhibits adipocyte differentiation through modulation of mitotic clonal expansion

Cited by 137 publications

References 43 publications

Effects of Ectopic Expression of Transcription Factors on Adipogenic Transdifferentiation in Bovine Myoblasts

Effects of Ectopic Expression of Transcription Factors on Adipogenic Transdifferentiation in Bovine Myoblasts

Rohitukine inhibits in vitro adipogenesis arresting mitotic clonal expansion and improves dyslipidemia in vivo

Studies on Mechanistic Role of Natural Bioactive Compounds in the Management of Obesity An Overview

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