Curcumin induces osteoblast differentiation through mild-endoplasmic reticulum stress-mediated such as BMP2 on osteoblast cells

Son, Hyo-Eun; Kim, Eun-Jung; Jang, Won‐Gu

doi:10.1016/j.lfs.2017.12.008

Cited by 57 publications

(46 citation statements)

References 31 publications

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“…In addition, gene expression analysis of this study also showed in periodontally diseased sites that CURC therapy associated with INS or even alone, when compared to DM rats treated with placebo, promoted up‐regulation of Runx2, which is essential for skeletal development . Earlier in vitro investigations also demonstrated that CURC increased the expression of Runx2 . In accordance, in a study evaluating the protective effects of CURC on the bones of rats with dexamethasone‐induced osteoporosis, Chen et al reported that the bone‐protective effects of CURC were mediated through the regulation of Runx2.…”

Section: Discussionsupporting

confidence: 82%

“…42 Earlier in vitro investigations also demonstrated that CURC increased the expression of Runx2. 43,44 In accordance, in a study evaluating the protective effects of CURC on the bones of rats with dexamethasone-induced osteoporosis, Chen et al 41 reported that the bone-protective effects of CURC were mediated through the regulation of Runx2. Our gene expression outcomes also revealed that the use of CURC (alone or combined to INS), when compared to placebo therapy, encouraged up-regulation of SIRT, found persistently in reduced levels in DM patients 45 and associated with regulation of mitochondrial antioxidant defense.…”

Section: Discussionmentioning

confidence: 85%

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Impact of natural curcumin on the progression of experimental periodontitis in diabetic rats

Pimentel

Casati

Ribeiro

et al. 2019

J of Periodontal Research

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Objective To evaluate the role of natural curcumin (CURC) on experimental periodontitis (EP) in animals with diabetes mellitus (DM). Material and Methods One hundred rats were assigned to DM + placebo (PLA); DM + CURC; DM + insulin (INS); DM + CURC + INS; and Non‐DM. Diabetes was induced by streptozotocin. After 3 days, they were initiated CURC and PLAC solutions and insulin administrations, daily for 30 days. This included a period of 19 days prior to EP induction (ligature at the first mandibular and the second maxillary molar) and then additional 11 days. Specimens from the mandible were processed for morphometric examination of bone level. Gingival tissues from mandibular molars were collected for quantification of IL‐1β, IL‐4, IL‐6, IL‐17, IFN‐γ, and TNF‐α using a Luminex/MAGpix assay. Gingivae from maxillary molars were subjected to RT‐PCR for assessment of Runx2, RANKL, OPG, SIRT, Dkk1, and Sost levels. Results Lower linear bone loss was detected in ligated molars of DM + CURC + INS vs DM + PLAC and DM + INS groups (P < 0.05). In ligated sites from DM rats treated with CURC + INS, IL‐6, IL‐1β, INF‐γ, and TNF‐α levels were the lowest in comparison with PLAC and/or INS and CURC as monotherapies (P < 0.05). CURC, independently of INS, increased Runx2 and SIRT when compared to DM + PLAC (P < 0.05) in ligated sites, whereas only CURC + INS reduced the RANKL/OPG ratio when compared to DM + PLAC (P < 0.05). Conclusion Natural CURC, when associated with INS, reduces the DM‐induced loss of supporting alveolar bone and promotes favorable modulation on osteo‐immune‐inflammatory mediators.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 85%

Impact of natural curcumin on the progression of experimental periodontitis in diabetic rats

Pimentel

Casati

Ribeiro

et al. 2019

J of Periodontal Research

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“…Induction of ALP expression and upregulation of ALP activity have been considered as important biological markers for osteoblastic activity, bone differentiation and mineralization . Here, our results indicate that Orexin‐A increased the activity of ALP and the gene expression of ALP as well as the expression of OCN, Osx, and Col‐I.…”

Section: Discussionsupporting

confidence: 62%

Orexins Facilitates Osteogenic Differentiation of MC3T3‐E1 Cells

2018

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Dysfunction of osteoblastic bone formation and matrix mineralization plays a key role in the pathological development of osteoporosis. The orexin peptide orexin-A, a highly excitatory neuropeptide hormone, possesses various biological functions by activating its specific G protein-coupled receptors, orexin-1 receptor (OX1R) and orexin-2 receptor (OX2R). Here, we report that OX1R but not OX2R was expressed in MC3T3-E1 cells. Importantly, we found that orexin-A accelerated osteoblast differentiation and matrix mineralization in MC3T3-E1 cells, as manifested by elevation of physiological markers of osteoblastic differentiation [alkaline phosphatase (ALP) and osteogenic genes] and Alizarin Red staining, respectively. Importantly, our findings indicated that orexin-A significantly increased the expression of runt-related transcription factor 2 (Runx-2), which is the central transcriptional factor. Orexin-A treatment phosphorylated the kinase p38 mitogen-activated protein kinase (MAPK) in a dose- and time-dependent manner. Also, orexin-induced increase in gene expression (Runx-2, ALP, osteocalcin, and osterix) and matrix mineralization were prevented by the p38 MAPK specific inhibitor SB203580. Additionally, we also revealed that protein kinase D (PKD) is involved in the effects of Orexin-A on p38 MAPK activation and Runx-2 expression. Finally, we found that Orexin-A-induced osteoblastic formation and matrix mineralization and the activation of the PKD/p38 MAPK pathway are mediated by OX1R. Based on these findings, we concluded that activation of OX1R by orexin-A might possess a therapeutic strategy for bone disease. © 2018 IUBMB Life, 70(7):633-641, 2018.

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“…There are several reports concerning the effects of curcumin on the differentiation, activity and death of osteoclasts and osteoblasts (Son et al, 2018). Bone homeostasis is maintained by osteoclast mediated bone resorption and osteoblas t mediated bone formation.…”

Section: Discussionmentioning

confidence: 99%

The ameliorative role of curcumin on the morphological and skeletal malformations induced by betamethasone in rat fetuses

Badawy¹,

Sakr²,

El–Borm³

2018

Egypt. J. Exp. Biol. (Zoo.)

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ABSTRACT:Among the glucocorticoids, betamethasone has become very popular, being more commonly used for several diseases, to promote organ maturation and to prevent preterm delivery. This work aimed to examining the possible morphological and skeletal malformations induced by betamethasone in foetuses of albino rats an d the role of curcumin as antioxidant against these adverse effects. Administration of betamethasone during the organogenesis phase at a dose equivalent to its human counterpart led to severe intrauterine growth retardation relative to the control group as it affected the growth parameters of the foetuses including reduction in length and weight. In addition, various malformations were detected in the skin, head, trunk, limbs and tail. Exencephaly, mandibular hypoplasia, and retarded ossification were the m ost evident endo-skeletal malformations observed in the foetuses maternally injected with betamethasone. A marked improvement in foetal growth in terms of shape, length, weight, and skeleton was recorded in foetuses maternally injected with betamethasone followed by curcumin.

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Curcumin induces osteoblast differentiation through mild-endoplasmic reticulum stress-mediated such as BMP2 on osteoblast cells

Cited by 57 publications

References 31 publications

Impact of natural curcumin on the progression of experimental periodontitis in diabetic rats

Impact of natural curcumin on the progression of experimental periodontitis in diabetic rats

Orexins Facilitates Osteogenic Differentiation of MC3T3‐E1 Cells

The ameliorative role of curcumin on the morphological and skeletal malformations induced by betamethasone in rat fetuses

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