Curcumin induced HepG2 cell apoptosis-associated mitochondrial membrane potential and intracellular free Ca2+ concentration

Wang, Mu; Ruan, Yuxia; Qian, Chen; Li, Shengpu; Wang, Qiulan; Cai, Jiye

doi:10.1016/j.ejphar.2010.09.049

Cited by 117 publications

(87 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…21 Curcumin (Ccn), a hydrophobic polyphenol structure, is extracted from the rhizomes of Curcuma longa 22 and has a number of medicinal and therapeutic properties, including anti-inflammatory, antiseptic, antioxidative, and hypolipidemic effects. 23,24 Moreover, emerging evidence shows that Ccn might induce apoptosis or cell cycle arrest in several types of cancer. Non-cytotoxic to healthy cells, [25][26][27] Ccn can also serve as a drug or be an adjunct to traditional chemotherapy in the treatment of cancer.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and in vitro evaluation of curcumin-loaded albumin nanoparticles surface-functionalized with glycyrrhetinic acid

Li¹,

Chen²,

Deng³

et al. 2015

IJN

View full text Add to dashboard Cite

We have designed and developed curcumin (Ccn)-loaded albumin nanoparticles (BNPs) surface-functionalized with glycyrrhetinic acid (Ccn-BNP-GA) for GA receptor-mediated targeting. Ccn-BNP-GA was prepared by conjugating GA as a hepatoma cell-specific binding molecule onto the surface of BNPs. Ccn-BNP-GA showed a narrow distribution with an average size of 258.8±6.4 nm, a regularly spherical shape, an entrapment efficiency of 88.55%±5.54%, and drug loading of 25.30%±1.58%. The density of GA as the ligand conjugated to BNPs was 140.48±2.784 μg/g bovine serum albumin. Cytotoxicity assay results indicated that Ccn-BNP-GA was significantly more cytotoxic to HepG2 cells and in a concentration-dependent manner. Ccn-BNP-GA also appeared to be taken up to a greater extent by HepG2 cells than undecorated groups, which might be due to the high affinity of GA for GA receptors on the HepG2 cell surface. These cytotoxicity assay results were corroborated by analysis of cell apoptosis and the cell cycle. Further, Ccn-BNP-GA showed an approximately twofold higher rate of cell apoptosis than the other groups. Moreover, proliferation of HepG2 cells was arrested in G 2 /M phase based on cell cycle analysis. These results, which were supported by the GA receptor-mediated endocytosis mechanism, indicate that BNPs surface-functionalized with GA could be used in targeted cancer treatment with high efficacy, sufficient targeting, and reduced toxicity.

show abstract

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and in vitro evaluation of curcumin-loaded albumin nanoparticles surface-functionalized with glycyrrhetinic acid

Li¹,

Chen²,

Deng³

et al. 2015

IJN

View full text Add to dashboard Cite

show abstract

“…Although curcumin (4) has chemopreventive properties due to its antioxidant activities, it is well documented that curcumin (4) can act as a prooxidant and anti-proliferative agent via causing mitochondrial dysfunction under certain conditions (Bhaumik et al, 1999;Galati et al, 2002;Wang et al, 2011). Using a clonogenic survival assay and MTT assay, pretreatment with curcumin (4) caused sensitivity to IR in cervical cancer cell lines, SiHa and HeLa, in contrast with radioprotective effects in normal diploid fibroblast (MRC-5) cells after treatment with curcumin (4) and IR.…”

Section: Curcuminmentioning

confidence: 99%

Phenylpropanoids in radioregulation: double edged sword

2011

View full text Add to dashboard Cite

Radiotherapy, frequently used for treatment of solid tumors, carries two main obstacles including acquired radioresistance in cancer cells during radiotherapy and normal tissue injury. Phenylpropanoids, which are naturally occurring phytochemicals found in plants, have been identified as potential radiotherapeutic agents due to their anti-cancer activity and relatively safe levels of cytotoxicity. Various studies have proposed that these compounds could not only sensitize cancer cells to radiation resulting in inhibition of growth and cell death but also protect normal cells against radiation-induced damage. This review is intended to provide an overview of recent investigations on the usage of phenylpropanoids in combination with radiotherapy in cancer treatment.

show abstract

“…The combined treatment mainly induced cell apoptosis. The morphological changes implied that some physiological changes could occur in membrane proteins, including the opening and closing of ion channels, the disruption of the ion channel structure, and alterations to the chemical composition of the outer membrane [30]. The altered chemical composition of the outer membrane may have led to changes in the ultrastructure of the cytoskeleton and membrane surface.…”

Section: Cell Morphology and Cell Membrane Ultrastructure Characterizmentioning

confidence: 99%

Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

et al. 2013

Self Cite

View full text Add to dashboard Cite

When used in combination with certain chemotherapies, curcumin has been shown to increase apoptosis in several cancer cell lines. Here, we report the combined effects of curcumin and cinobufacini on human cervical carcinoma cells. The aim of this study was to examine whether curcumin could enhance apoptosis induced by cinobufacini. 3-(4,5-Dimethylthiazol-2-y1)-2,5-diphenytetrazolium bromide (MTT) assays revealed that the growth and proliferation of HeLa cells could be inhibited by 75% after a combined treatment of 25 μg/mL cinobufacini and 8 μg/mL curcumin. The combined treatment is 3 times more effective than treatment with 25 μg/mL cinobufacini alone. Annexin V-FITC/PI staining, morphological changes and immunofluorescence verified a significant enhancement in cinobufacini-induced apoptosis when cells were also exposed to curcumin. The data showed that the proportion of early apoptotic cells significantly increased from 15.43% in cells treated only with 25 μg/mL cinobufacini to 49.2% in cells treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. Moreover, compared with treatment of only 25 μg/mL cinobufacini, ROS production increased 1.7-fold, the intracellular free Ca 2+ concentration increased 1.5-fold, and the mitochondrial membrane potential decreased by 20% in the combined treatment. Simultaneously, the atomic force microscopy (AFM) results suggest that cells treated with a combination of cinobufacini and curcumin varied significantly in shape and ultrastructure. Collapsed cells with leaking cytoplasm, blebbing pores and emerging apoptotic bodies were prevalent. The nanoparticle size increased from 70 nm when the cells were treated with 25 μg/mL cinobufacini to 190 nm when the cells were treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. The size increase resulted in the cell membrane becoming considerably rough. These results can improve our understanding of combination treatments. Specifically, the combination of cinobufacini and curcumin may potentially find use as a novel cervical carcinoma treatment. Additionally, AFM is a powerful tool that can be used to explore cellular morphologies and ultrastructures. In this study, we combined cinobufacini (CIN) and curcumin (CUR) to treat cervical carcinoma. Recently, several traditional Chinese medicines with anti-tumor properties have attracted considerable interest as candidates for novel cancer therapeutics [7]. CIN is a watersoluble extract prepared from the skins of Bufo bufo gargarizans. Recent clinical studies have suggested that CIN,

show abstract

Curcumin induced HepG2 cell apoptosis-associated mitochondrial membrane potential and intracellular free Ca2+ concentration

Cited by 117 publications

References 38 publications

Synthesis, characterization, and in vitro evaluation of curcumin-loaded albumin nanoparticles surface-functionalized with glycyrrhetinic acid

Synthesis, characterization, and in vitro evaluation of curcumin-loaded albumin nanoparticles surface-functionalized with glycyrrhetinic acid

Phenylpropanoids in radioregulation: double edged sword

Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

Contact Info

Product

Resources

About