Curcumin-Functionalized Gelatin Films: Antioxidant Materials with Modulated Physico-Chemical Properties

Rubini, Katia; Boanini, Elisa; Parmeggiani, Silvia; Bigi, Adriana

doi:10.3390/polym13111824

Cited by 12 publications

(4 citation statements)

References 30 publications

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“…Using poly-caprolactone as a sustained-release polymer extended the release of curcumin [ 33 ]. In a study by Rubini et al, curcumin-containing gelatin films exhibit sustained curcumin release due to the existence of glutardialdehyde [ 34 ]. In our study, glutardialdehyde (1%) was used for gelatin cross-linking.…”

Section: Discussionmentioning

confidence: 99%

Gelatin–Curcumin Nanocomposites as a Coating for Implant Healing Abutment: In Vitro Stability Investigation

Dizaj

Torab

Kouhkani

et al. 2023

Clinics and Practice

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Regarding the importance of preventing peri-implantitis in dental implants, the current study aimed to coat a healing abutment with gelatin–curcumin nanocomposites, and the stability of this coating on the healing abutment was evaluated. A cell viability measuring test was used to determine the cytotoxicity of nanocomposites against dental pulp stem cells. To show the pattern of curcumin release from nanocomposites, drug dissolution apparatus two was applied. Then, 16 healing abutments were examined in vitro. Titanium healing abutments were coated with the gelatin–curcumin nanocomposite. The dip coating method was applied for coating and the consistency of coated cases was evaluated at intervals of one, 30, and 60 days after coating inside the simulated body fluid (SBF) solution. A scanning electron microscope (SEM) was used for investigating the microstructure and morphology of coatings, and an energy dispersive X-ray (EDX) was applied for determining the combination of the coating. Moreover, the healings were weighed before and after coating via an accurate digital scale with an accuracy of 0.0001. Finally, the data were analyzed using SPSS software. The prepared nanocomposite was non-cytotoxic against tested cells. The nanocomposite showed a relatively rapid release pattern in the first 10 days for curcumin. The release of curcumin from the nanoparticles continued slowly until the 30th day. The weight changes were statistically significant (p-value < 0.001) during this time. Based on the post hoc test, the weight between two times immediately after coating and 30 days after coating, and also one day after coating and 30 days after coating, was statistically insignificant. The results revealed that the coating of the gelatin–curcumin nanocomposite on the healing was successful and this consistency was kept for at least one month. It is necessary to investigate more evaluations in different fields of physicochemical, mechanical, and antimicrobial aspects for coated healing abutments.

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Section: Discussionmentioning

confidence: 99%

Gelatin–Curcumin Nanocomposites as a Coating for Implant Healing Abutment: In Vitro Stability Investigation

Dizaj

Torab

Kouhkani

et al. 2023

Clinics and Practice

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“…Gelatin world production is impressive; the annual amount is reported to reach up to 326,000 tons [ 2 ], and it is expected to increase significantly in the future [ 3 ]. The high interest in this hydrocolloid is due to its unique properties: it is biocompatible, biodegradable, not antigenic, abundant, and relatively cheap, and it can be easily used to form films and coatings [ 4 , 5 , 6 ]. Its numerous applications span from the biomedical and pharmaceutical fields to food processing and packaging [ 3 , 4 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of WO3 Nanoparticles on the Properties of Gelatin Films

Rubini,

Menichetti,

Cassani

et al. 2024

Gels

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Gelatin films are very versatile materials whose properties can be tuned through functionalization with different systems. This work investigates the influence of WO3 nanoparticles on the swelling, barrier, mechanical, and photochromic properties of gelatin films. To this purpose, polyvinylpirrolidone (PVP)-stabilized WO3 nanoparticles were loaded on gelatin films at two different pH values, namely, 4 and 7. The values of swelling and solubility of functionalized films displayed a reduction of around 50% in comparison to those of pristine, unloaded films. In agreement, WO3 nanoparticles provoked a significant decrease in water vapor permeability, whereas the decrease in the values of elastic modulus (from about 2.0 to 0.7 MPa) and stress at break (from about 2.5 to 1.4 MPa) can be ascribed to the discontinuity created by the nanoparticles inside the films. The results of differential scanning calorimetry and X-ray diffraction analysis suggest that interaction of PVP with gelatin reduce gelatin renaturation. No significant differences were found between the samples prepared at pH 4 and 7, whereas crosslinking with glutaraldehyde greatly influenced the properties of gelatin films. Moreover, the incorporation of WO3 nanoparticles in gelatin films, especially in the absence of glutaraldehyde, conferred excellent photochromic properties, inducing the appearance of an intense blue color after a few seconds of light irradiation and providing good resistance to several irradiation cycles.

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“…Glutaraldehyde (GTA) is by far the most used crosslinking molecule in biomedicine because it can efficiently stabilize collagen or its derivatives in an aqueous environment. Crosslinking of collagen or its analogue with GTA involves reactions between the free amine groups of lysine or hydroxylysine amino acid residues in the collagenous polypeptide chains and the aldehyde groups of GTA to form imine bonds [17,18]. However, GTA is cytotoxic due to slow release from the scaffold.…”

Section: Introductionmentioning

confidence: 99%

Gelatin scaffold ameliorates proliferation & stem cell differentiation into the hepatic like cell and support liver regeneration in partial-hepatectomized mice model

Kumari,

Tandon,

Ghosh

et al. 2023

Biomed. Mater.

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Stem cell-based tissue engineering is an emerging tool for developing functional tissues of choice. To understand pluripotency and hepatic differentiation of mouse embryonic stem cells (mESCs) on a three-dimensional (3D) scaffold, we established an efficient approach for generating hepatocyte-like cells from hepatoblast cells. We developed porous and biodegradable scaffold, which was stimulated with exogenous growth factors and investigated stemness and differentiation capacity of mESCs into hepatocyte-like cells on the scaffold in -vitro. In animal studies, we had cultured mESCs-derived hepatoblast-like cells on the scaffold and then, transplanted them into the partially hepatectomized (PH) C57BL/6 male mice model to evaluate the effect of gelatin scaffold on hepatic regeneration. The 3D culture system allowed maintenance of stemness properties in mESCs. The step-wise induction of mESCs with differentiation factors leads to the formation of hepatocyte-like cells andexpressed liver-specific genes, including albumin, hepatocyte nucleic factor 4 alpha (HNF4α), Ki67 and cytokeratin 18 (CK18). In addition, cells also expressed Ki67, indicating cells are proliferating. The secretome showed expression of albumin, urea, creatinine, alanine transaminase (ALT), and aspartate aminotransferase (AST).. However, the volume of the excised liver which aids regeneration has not been studied. Our results indicate that hepatoblast cells on the scaffold implanted in PH mouse indicates that these cells efficiently differentiate into hepatocyte-like cells and cholangiocytes, forming hepatic lobules with central and portal veins, and bile duct-like structures with neovascularization. The gelatin scaffold provides an efficient microenvironment for liver differentiation and regeneration both in-vitro and in-vivo. These hepatoblasts cells would be a valuable source for 3D liver tissue engineering/transplantation in liver diseases.

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Curcumin-Functionalized Gelatin Films: Antioxidant Materials with Modulated Physico-Chemical Properties

Cited by 12 publications

References 30 publications

Gelatin–Curcumin Nanocomposites as a Coating for Implant Healing Abutment: In Vitro Stability Investigation

Gelatin–Curcumin Nanocomposites as a Coating for Implant Healing Abutment: In Vitro Stability Investigation

The Role of WO3 Nanoparticles on the Properties of Gelatin Films

Gelatin scaffold ameliorates proliferation & stem cell differentiation into the hepatic like cell and support liver regeneration in partial-hepatectomized mice model

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