Curcumin exhibits anti-tumor effect and attenuates cellular migration via Slit-2 mediated down-regulation of SDF-1 and CXCR4 in endometrial adenocarcinoma cells

Sirohi, Vijay Kumar; Popli, Pooja; Sankhwar, Pushplata; Kaushal, Jyoti; Gupta, Kanchan; Manohar, Murli; Dwivedi, Anila

doi:10.1016/j.jnutbio.2016.12.021

Cited by 46 publications

(29 citation statements)

References 47 publications

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“…TEP has about 17 times higher curcumin content than nanoemulsion. Since curcumin can induce cell death according to its concentration, as well as exhibit various pharmacological activities [ 30 ] (e.g., antioxidation [ 27 , 31 , 32 ], antiinflammation [ 33 , 34 ], and antitumor effects [ 35 , 36 , 37 ]), our results showing cytotoxicity at high TEP with a high curcumin content can be explained. Furthermore, based on the No Observed Adverse Effect Level (NOAEL) value of curcumin (250–320 mg/kg bw/day), international expert scientific committee JECFA (joint FAO/WHO Expert Committee on Food Additives) have proposed an acceptable daily intake (ADI) of 3 mg/kg bw/day for insoluble curcumin.…”

Section: Discussionmentioning

confidence: 85%

Cytotoxicity Evaluation of Turmeric Extract Incorporated Oil-in-Water Nanoemulsion

Yoon

Zhang

Kang

et al. 2018

IJMS

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To overcome the drawbacks of conventional drug delivery system, nanoemulsion have been developed as an advanced form for improving the delivery of active ingredients. However, safety evaluation is crucial during the development stage before the commercialization. Therefore, the aim of this study was to evaluate the cytotoxicity of two types of newly developed nanoemulsions. Turmeric extract-loaded nanoemulsion powder-10.6 (TE-NEP-10.6, high content of artificial surfactant Tween 80), which forms the optimal nanoemulsion, and the TE-NEP-8.6 made by increasing the content of natural emulsifier (lecithin) to reduce the potential toxicity of nanoemulsion were cultured with various cells (NIH3T3, H9C2, HepG2, hCPC, and hEPC) and the changes of each cell were observed followed by nanoemulsion treatment. As a result, the two nanoemulsions (TE-NEP-10.6 and TE-NEP-8.6) did not show significant difference in cell viability. In the case of cell line (NIH3T3, H9C2, and HepG2), toxicity was not observed at an experimental concentration of less than 1 mg/mL, however, the cell survival rate decreased in a concentration dependent manner in the case of primary cultured cells. These results from our study can be used as a basic data to confirm the cell type dependent toxicity of nanoemulsion.

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Section: Discussionmentioning

confidence: 85%

Cytotoxicity Evaluation of Turmeric Extract Incorporated Oil-in-Water Nanoemulsion

Yoon

Zhang

Kang

et al. 2018

IJMS

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“…Curcumin is a polyphenolic phytochemical compound, and it has multiple anti-cancer effects. For example, curcumin promotes apoptosis in several types of cancer cells [ 7 – 10 ] and inhibits migration [ 11 , 12 ] and angiogenesis [ 13 ]. Furthermore, curcumin enhances the cell death of cancer cells by anti-cancer drugs treatment, including TRAIL [ 14 – 16 ], 5-fluorouracil and gemcitabine [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

mTORC1/2 inhibitor and curcumin induce apoptosis through lysosomal membrane permeabilization-mediated autophagy

et al. 2018

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mTOR is an important regulator of cell growth and forms two complexes, mTORC1/2. In cancer, mTOR signaling is highly activated, and the regulation of this signaling, as an anti-cancer strategy, has been emphasized. However, PP242 (inhibitor of mTORC1 and mTORC2) alone did not induce human renal carcinoma cell death. In this study, we found that PP242 alone did not alter cell viability, but combined curcumin and PP242 treatment induced cell death. Combined PP242 and curcumin treatment induced Bax activation and decreased expression of Mcl-1 and Bcl-2. Furthermore, co-treatment with PP242 and curcumin-induced the downregulation of the Rictor (an mTORC2 complex protein) and Akt protein levels, and ectopic overexpression of Rictor or Akt inhibited PP242 plus curcumin induced cell death. Downregulation of Rictor increased cytosolic Ca2+ release from endoplasmic reticulum, which led to lysosomal damage in PP242 plus curcumin-treated cells. Furthermore, damaged lysosomes induced autophagy. Autophagy inhibitors markedly inhibited cell death. Finally, combined curcumin and PP242 treatment reduced tumor growth and induced cell death in xenograft models. Altogether, our results reveal that combined PP242 and curcumin treatment could induce autophagy-mediated cell death by reducing the expression of Rictor and Akt in renal carcinoma cells.

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“…[17][18][19] For example, it is reported that curcumin shows antiproliferative activities in tumor cells via induction of cell apoptosis mediated by various signaling pathways, such as p53-dependent apoptosis pathway and deregulation of cyclin D1 expression. 20,21 Moreover, curcumin has been shown to play neuroprotection roles in homocysteine rat model of Parkinson's disease and amyloid-βinduced mitochondrial dysfunction through scavenging oxygen species and preventing thiol group oxidation. 22,23 Although the ability to kill tumor cells makes curcumin an attractive drug candidate, its application is strictly limited due to its poor water solubility and low bioavailability.…”

Section: Introductionmentioning

confidence: 99%

Targeted therapy of intracranial glioma model mice with curcumin nanoliposomes

Zhao¹,

Zhao²,

Fu³

et al. 2018

IJN

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BackgroundGlioma is the most aggressive and lethal brain tumor in humans, it comprises about 30 per cent of all brain tumors and central nervous system tumors.PurposeThe objective of this study was to create novel brain-targeting nanoliposomes to encapsulate curcumin as a promising option for glioma therapy.Patients and methodsHuman glioma cells (U251MG) were used to determine cell uptake efficiency and possible internalization mechanism of the curcumin-loaded nanoliposomes modified by a brain-targeting peptide RDP. In addition, intracranial glioma mice model was prepared by transplantation of U251MG cells into the mice striatum, and then the liposomes were intravenously administered into the glioma-bearing mice to evaluate the anti-glioma activity.ResultsRDP-modified liposomes (RCL) could enter the brain and glioma region, and were internalized by the glioma cells perhaps through acetylcholine receptor-mediated endocytosis pathway. Furthermore, the RCL prolonged the survival time of the glioma-bearing mice from 23 to 33 days, and the inhibition mechanism of the RCL on glioma cell was partly due to cell cycle arrest at the S phase and induction of cell apoptosis.ConclusionThis study would provide a potential approach for targeted delivery of drug-loaded liposomes for glioma treatment.

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Curcumin exhibits anti-tumor effect and attenuates cellular migration via Slit-2 mediated down-regulation of SDF-1 and CXCR4 in endometrial adenocarcinoma cells

Cited by 46 publications

References 47 publications

Cytotoxicity Evaluation of Turmeric Extract Incorporated Oil-in-Water Nanoemulsion

Cytotoxicity Evaluation of Turmeric Extract Incorporated Oil-in-Water Nanoemulsion

mTORC1/2 inhibitor and curcumin induce apoptosis through lysosomal membrane permeabilization-mediated autophagy

Targeted therapy of intracranial glioma model mice with curcumin nanoliposomes

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