Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression

El‐Far, Ali H.; Saddiq, Amna A.; Mohamed, Shymaa A; Almaghrabi, Omar A.; Mousa, Shaker A.

doi:10.1177/15347354221099537

Cited by 8 publications

(8 citation statements)

References 48 publications

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“…[20][21][22][23][24] Recent studies have highlighted curcumin's antiproliferative and antimotility effects on breast cancer cells, yet the intricate molecular mechanisms underlying these actions require further elucidation. [25][26][27] Therefore, exploring curcumin's influence on cell motility, proliferation, cell cycle, and apoptosis, particularly in TNBC, and uncovering its molecular underpinnings are of paramount clinical relevance.…”

Section: Discussionmentioning

confidence: 99%

Curcumin suppresses metastasis of triple-negative breast cancer cells by modulating EMT signaling pathways: An integrated study of bioinformatics analysis

Chen,

Lu,

et al. 2024

Medicine

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This study aimed to use bioinformatics approaches for predicting the anticancer mechanisms of curcumin on triple-negative breast cancer (TNBC) and to verify these predictions through in vitro experiments. Initially, the Cell Counting Kit-8 (CCK8) assay was employed to rigorously investigate the influence of curcumin on the proliferative capacity of TNBC cells. Subsequently, flow cytometry was employed to meticulously assess the impact of curcumin on cellular apoptosis and the cell cycle regulation. Transwell assays were employed to meticulously evaluate the effect of curcumin on the motility of TNBC cells. RNA sequencing was conducted, followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of differentially expressed genes, aiming to elucidate the potential anticancer mechanisms underlying curcumin’s effects. To thoroughly elucidate the interactions among multiple proteins, we constructed a protein–protein interaction (PPI) network. Finally, the expression levels of several key proteins, including fibronectin, mTOR, β-Catenin, p-Akt, Akt, N-Cadherin, p-S6, and S6, were assessed using the western blot. The CCK8 assay results showed that curcumin significantly inhibited the proliferation of Hs578T and MDA-MB-231 cells. Flow cytometry results showed that curcumin induced apoptosis in these cells and arrested the cell cycle at the G2/M phase. Additionally, Transwell assay results showed that curcumin effectively reduced the motility of Hs578T and MDA-MB-231 cells. Enrichment analysis of RNA sequencing data showed that the mechanism of action of curcumin was significantly associated with signaling pathways such as pathways in cancer, focal adhesion, and PI3K-Akt signaling pathways. Subsequently, we constructed a protein–protein interaction network to elucidate the interactions among multiple proteins. Finally, Western blotting analysis showed that curcumin significantly decreased the expression levels of key proteins including Fibronectin, mTOR, β-Catenin, p-Akt, Akt, N-Cadherin, p-S6, and S6. Curcumin exhibits its therapeutic potential in TNBC by modulating multiple signaling pathways. It may inhibit the epithelial-mesenchymal transition process by downregulating the expression of proteins involved in the mTOR and PI3K-Akt signaling pathways, thereby suppressing the motility of TNBC cells. These findings provide experimental evidence for considering curcumin as a potential therapeutic strategy in the treatment of TNBC.

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Section: Discussionmentioning

confidence: 99%

Curcumin suppresses metastasis of triple-negative breast cancer cells by modulating EMT signaling pathways: An integrated study of bioinformatics analysis

Chen,

Lu,

et al. 2024

Medicine

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“…Data suggest that proteasome-mediated downregulation of cyclin E and upregulation of CDC inhibitors contribute to the antiproliferative effects of CUR [ 58 ]. A combination of CUR and thymoquinone against BC decreased caspase-3, phosphatidylinositol 3-kinase (PI3K), and protein kinase B (AKT) protein levels, which is strongly related to P21 [ 59 ] ( Figure 3 ).…”

Section: Curcumin Biogenesis In the Regulation Of Breast Cancermentioning

confidence: 99%

Curcumin for Treating Breast Cancer: A Review of Molecular Mechanisms, Combinations with Anticancer Drugs, and Nanosystems

Zhu,

Li,

et al. 2024

Pharmaceutics

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Breast cancer (BC) has become the fifth most prevalent cause of cancer-related morbidity, attracting significant attention from researchers due to its heightened malignancy and drug resistance. Conventional chemotherapy approaches have proven inadequate in addressing all BC subtypes, highlighting the urgent need for novel therapeutic approaches or drugs. Curcumin (CUR), a phytochemical derived from Curcuma longa (turmeric), has shown substantial potential in inhibiting BC cell migration, metastasis, and proliferation. However, the use of CUR in this context comes with challenges due to its dynamic and easily degradable nature, poor aqueous solubility, low bioavailability, rapid metabolism, and swift systemic elimination, collectively limiting its clinical applications. As such, we provide an overview of the properties, synthesis, and characterization of the hybridization of CUR and its analogue with chemo-drug building blocks. We reviewed research from the last five years on CUR’s biogenesis with respect to the regulation of BC, revealing that CUR participates in arresting BC cells in the cell cycle and significantly induces apoptosis in BC cells. Information on the chemotherapeutic and antitumor mechanisms of CUR in BC, including regulation of the cell cycle, increased cell apoptosis, and inhibition of multidrug resistance (MDR), was compiled. Additionally, we provide an overview of CUR loaded into nanomaterials that are cotreated with other chemotherapeutic drugs, such as paclitaxel, thymoquinone, and tamoxifen. In this review, we discuss different types of nanoparticles that can be used for CUR delivery, such as polymeric nanoparticles, carbon nanotubes, and liposomes. By comparing the size, entrapment efficiency, drug-loading capacity, release time, biocompatibility, pharmaceutical scale, and reproducibility of various nanomaterials, we aimed to determine which formulations are better suited for loading CUR or its analogue. Ultimately, this review is expected to offer inspiring ideas, promising strategies, and potential pathways for developing advanced anti-BC strategy nanosystems in clinical practice.

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“…Due to inducing the expression of miR-34a, the tumor growth and invasion had suppressed [ 55 ]. Another study examined the synergistic effect of curcumin and thymoquinone (TQ), on the development of MCF7 and MDA-MB-231 breast cancer cell lines [ 56 ]. Moreover, this compound and gemcitabine prevented the development, invasion, and metastasis of the pancreatic cancer orthotopic model.…”

Section: Combination Therapies Based On Selected Natural Productsmentioning

confidence: 99%

Combination Anticancer Therapies Using Selected Phytochemicals

et al. 2022

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Cancer is still one of the most widespread diseases globally, it is considered a vital health challenge worldwide and one of the main barriers to long life expectancy. Due to the potential toxicity and lack of selectivity of conventional chemotherapeutic agents, discovering alternative treatments is a top priority. Plant-derived natural products have high potential in cancer treatment due to their multiple mechanisms of action, diversity in structure, availability in nature, and relatively low toxicity. In this review, the anticancer mechanisms of the most common phytochemicals were analyzed. Furthermore, a detailed discussion of the anticancer effect of combinations consisting of natural product or natural products with chemotherapeutic drugs was provided. This review should provide a strong platform for researchers and clinicians to improve basic and clinical research in the development of alternative anticancer medicines.

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Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression

Cited by 8 publications

References 48 publications

Curcumin suppresses metastasis of triple-negative breast cancer cells by modulating EMT signaling pathways: An integrated study of bioinformatics analysis

Curcumin suppresses metastasis of triple-negative breast cancer cells by modulating EMT signaling pathways: An integrated study of bioinformatics analysis

Curcumin for Treating Breast Cancer: A Review of Molecular Mechanisms, Combinations with Anticancer Drugs, and Nanosystems

Combination Anticancer Therapies Using Selected Phytochemicals

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