Curcumin and resveratrol in combination modulates benzo(a)pyrene-induced genotoxicity during lung carcinogenesis

Abstract

The present study attempted to explore the efficacy of curcumin and resveratrol in modulating mitotic catastrophe and apoptosis during lung carcinogenesis. The mice were segregated into five groups, which included normal control, benzo(a)pyrene (BP)-treated, BP + curcumin (C)-treated, BP + resveratrol (R)-treated and BP + C + R-treated groups. The BP treatment resulted in a significant increase in the formation of micronuclei as well as in the protein expression of bcl-2 in the lungs of mice. On the other hand, a significant decrease was observed in the number of apoptotic cells and protein expression of bax in the lungs of BP-treated mice. Supplementation of curcumin and resveratrol individually to BP-treated animals resulted in a decrease in the micronuclei formation; however, it was not statistically significant. Interestingly, combination of curcumin and resveratrol resulted in a statistically significant decrease in micronuclei formation. Moreover, phytochemicals in combination significantly reduced the protein expression of bcl-2 in BP-treated mice. Furthermore, supplementation of phytochemicals in combination brought a noticeable improvement in the number of apoptotic cells as well as in the protein expression of bax. The present study, therefore, concludes that the combined treatment with curcumin and resveratrol modulates mitotic catastrophe by stimulating apoptosis in BP-treated mice.

Comparative Insilico Docking Analysis of Curcumin and Resveratrol on Breast Cancer Proteins and their Synergistic Effect on MCF-7 Cell Line

“…7 The Synergistic potential of curcumin and resveratrol in declining the active tumour suppressor protein in the lungs of Benzo[a]pyrene treated mice were reported earlier. 8 In vitro cell line study and in vivo study in mice for prostatic adenocarcinoma using curcumin and resveratrol drug combination induced apoptosis and decreased cell growth. 9 Enhancement of curcumin activity in the presence of resveratrol is reported against head and neck carcinoma.…”

Resveratrol and curcumin synergistically induces apoptosis in cigarette smoke condensate transformed breast epithelial cells through a p21Waf1/Cip1 mediated inhibition of Hh-Gli signaling

“…There are also reports where combinatorial effect of dietary anticancer agents against lung and liver carcinoma was explored (Malhotra et al, 2012;Du et al, 2013). In the context of breast cancer, it has already been demonstrated that combination of two or more drugs has an improved molecular action against different types and stages of breast cancers (Kim et al, 2013;Nasiri et al, 2013).…”

Study to Evaluate Molecular Mechanics behind Synergistic Chemo-Preventive Effects of Curcumin and Resveratrol during Lung Carcinogenesis

“…Phytochemicals are non nutritive products of plants and are presently being studied the world over for their chemopreventive actions in controlling various diseases including cancer. Amongst various chemo-preventive agents, phytochemicals especially curcumin and resveratrol in combination have shown great potential in combating cancer as observed in our previous studies [5]–[9]. However, there is paucity of information with regard to molecular mechanics behind these synergistic effects of curcumin and resveratrol.…”