Curcumin ameliorates PRMT5-MEP50 arginine methyltransferase expression by decreasing the Sp1 and NF-YA transcription factors in the A549 and MCF-7 cells

Chatterjee, Biji; Ghosh, Krishna; Suresh, Lavanya; Kanade, Santosh R.

doi:10.1007/s11010-018-3471-0

Cited by 28 publications

(18 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The positive association between altered or overexpressed PRMT5, EZH2, or KDACs and onset of cancer has prompted scientists to explore modulators of these enzymes that have the potential to be used as epigenetic therapeutics . Very recently, we have observed the similar modulatory action of another well‐studied natural molecule—curcumin, which reduced expression and function of PRMT5/MEP50 in vitro . In this study, we, for the first time, reporting the modulatory response of RVT toward oncogenes: PRMT5, EZH, and certain KDACs and KATs.…”

Section: Discussionmentioning

confidence: 78%

“…6,[11][12][13] Very recently, we have observed the similar modulatory action of another well-studied natural molecule-curcumin, which reduced expression and function of PRMT5/MEP50 in vitro. 32 In this study, we, for the first time, reporting the modulatory response of RVT toward oncogenes: PRMT5, EZH, and certain KDACs and KATs. RVT by affecting the expression of these vital epigenetic enzymes altered the global level of repressive and activating histone marks in both the cell lines under study.…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Resveratrol modulates epigenetic regulators of promoter histone methylation and acetylation that restores BRCA1, p53, p21^CIP1 in human breast cancer cell lines

2019

Self Cite

View full text Add to dashboard Cite

The epigenetic enzymes catalyze posttranslational modifications (PTMs) of histones, which functionally determine gene expression at the chromatin level. Resveratrol (RVT) a much studied anti‐cancer natural molecule is known for restoration of BRCA1, p53, and p21 in cancer cells. We aimed to investigate the role of histone methylation and acetylation on upregulation of these tumor suppressor genes. Our results suggest RVT significantly increase expression of BRCA1, p53, and p21, while decreased expression of protein arginine methyltransferase 5 (PRMT5) and enhancer of Zeste homolog 2 (EZH2) at a 20 μM concentration by 48 hr in both MCF‐7 and MDA‐MB‐231 breast cancer cells. Also, there was an overall loss of H4R3me2s (catalytic product of PRMT5) and H3K27me3 (catalytic product of PRMT5). In contrast, RVT exposure caused a significant decrease in lysine deacetylase (KDAC) activity and expression of KDAC1‐3, whereas the expression of lysine acetyltransferase KAT2A/3B was increased compared to the unexposed cells. As an outcome, RVT increased global level of H3K9ac and H3K27ac marks. The chromatin immunoprecipitation showed 20 μM RVT exposure significantly reduced the enrichment of repressive histone marks (H4R3me2s and H3K27me3) while the abundance of activating histone marks (H3K9/27ac) within the proximal promoter region of BRCA1, p53, and p21 was increased. We hypothesize RVT by affecting the expression and function of methylation and acetylation enzymes altered the epigenetic modifications on promoter histones that restored expression of these critically important tumor suppressor genes.

show abstract

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 96%

Resveratrol modulates epigenetic regulators of promoter histone methylation and acetylation that restores BRCA1, p53, p21^CIP1 in human breast cancer cell lines

2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Two major transcriptional pathways, namely CRE and Sp1mediated transcription, have been extensively studies in HD [53]. Increasing ndings demonstrate that several genes containing Sp1-binding motifs in their promoters are down-regulated in HD [55,77,78]. In the present study, one important nding was that Sp1 transactivated ZnT3 gene by binding the GC boxes in the promoter.…”

Section: Discussionmentioning

confidence: 47%

“…7A). Sp1, a key transcription factor isolated from human being, has been shown to recognize related GC motifs within promoter regions [55]. One GC-rich box (GC-1) in ZnT3 gene promoter is from − 269 bp to -261 bp, the other (GC-2) is from − 184 bp to -174 bp (Fig.…”

Section: Sp1 Positively Regulates Znt3 Expression Through Activating mentioning

confidence: 99%

Mutant huntingtin reduces vesicular zinc level by inhibiting the binding of Sp1 to ZnT3 promoter

Niu

Yang

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Synaptic dysfunction caused by mutant huntingtin greatly contributes to Huntington’s disease (HD) pathogenesis. HD patients show cognitive impairment as well as uncontrolled movements. Vesicular zinc is closely linked to modulating synaptic transmission and maintaining cognitive ability. However, whether does mutant huntingtin affect zinc homeostasis in the brain or not? This will be of great significance for further revealing the pathogenesis of HD. Methods N171-HD82Q transgenic mice and cultured BHK cells expressing N-terminal mutant huntingtin fragment containing 160 glutamines (160Q BHK cells) were used to investigate the effect of mutant huntingtin on zinc homeostasis and its molecular mechanisms. Results Herein, we have demonstrated that the density of synaptic vesicular zinc decreases in the cortex, striatum and hippocampus of N171-82Q mice. Given that vesicular zinc concentration depends on the abundance of zinc transporter 3 (ZnT3) on the membrane of synaptic vesicles, ZnT3 expression is detected in the brain of N171-82Q mice and 160Q BHK cells. Mutant huntingtin leads to a dramatical decrease in ZnT3 mRNA and protein levels in the three brain regions of these mice aged from 14 to 20 weeks. Significantly, Sp1 activates ZnT3 transcription via its binding to the GC boxes in ZnT3 promoter. Nevertheless, mutant huntingtin inhibits the binding of Sp1 to the promoter of ZnT3 gene and down-regulates ZnT3 expression. Furthermore, the overexpression of Sp1 ameliorates inhibition of ZnT3 gene transcription by mutant huntingtin. Conclusions Collectively, this first study to reveal a significant loss of synaptic vesicular zinc and ZnT3 expression caused by mutant huntingtin in the early stage of HD. Our findings have revealed the molecular mechanism underlying this change. Mutant huntingtin inhibits the binding of Sp1 to ZnT3 gene promoter to reduce ZnT3 expression. The imbalance of vesicular zinc homeostasis may be closely associated with synaptic dysfunction and cognitive deficits in HD. This work sheds novel mechanistic insights into the pathogenesis of HD and promises a potential therapeutic strategy for HD.

show abstract

“…Extensive research has revealed that Cur is an effective molecular agent with a broad spectrum of biological activities against aging, inflammation and cancer (6,7). Previous studies have reported that Cur inhibits proliferation, metastasis and initiation of several malignances, including lung, breast, hepatocellular, pancreatic and gastric cancers (8)(9)(10)(11)(12). In addition, epidemiological study has suggested that a diet rich in Cur may decrease the incidence of colon cancer (13).…”

Section: Introductionmentioning

confidence: 99%

Curcumin suppresses the proliferation of oral squamous cell carcinoma through a specificity protein 1/nuclear factor‑κB‑dependent pathway

Tian

2021

Exp Ther Med

View full text Add to dashboard Cite

Curcumin ameliorates PRMT5-MEP50 arginine methyltransferase expression by decreasing the Sp1 and NF-YA transcription factors in the A549 and MCF-7 cells

Cited by 28 publications

References 74 publications

Resveratrol modulates epigenetic regulators of promoter histone methylation and acetylation that restores BRCA1, p53, p21^CIP1 in human breast cancer cell lines

Resveratrol modulates epigenetic regulators of promoter histone methylation and acetylation that restores BRCA1, p53, p21^CIP1 in human breast cancer cell lines

Mutant huntingtin reduces vesicular zinc level by inhibiting the binding of Sp1 to ZnT3 promoter

Curcumin suppresses the proliferation of oral squamous cell carcinoma through a specificity protein 1/nuclear factor‑κB‑dependent pathway

Contact Info

Product

Resources

About

Curcumin ameliorates PRMT5-MEP50 arginine methyltransferase expression by decreasing the Sp1 and NF-YA transcription factors in the A549 and MCF-7 cells

Cited by 28 publications

References 74 publications

Resveratrol modulates epigenetic regulators of promoter histone methylation and acetylation that restores BRCA1, p53, p21CIP1 in human breast cancer cell lines

Resveratrol modulates epigenetic regulators of promoter histone methylation and acetylation that restores BRCA1, p53, p21CIP1 in human breast cancer cell lines

Mutant huntingtin reduces vesicular zinc level by inhibiting the binding of Sp1 to ZnT3 promoter

Curcumin suppresses the proliferation of oral squamous cell carcinoma through a specificity protein 1/nuclear factor‑κB‑dependent pathway

Contact Info

Product

Resources

About

Resveratrol modulates epigenetic regulators of promoter histone methylation and acetylation that restores BRCA1, p53, p21^CIP1 in human breast cancer cell lines

Resveratrol modulates epigenetic regulators of promoter histone methylation and acetylation that restores BRCA1, p53, p21^CIP1 in human breast cancer cell lines