Curcumin ameliorates alveolar bone destruction of experimental periodontitis by modulating osteoclast differentiation, activation and function

Mau, Lian Ping; Cheng, Wan Chien; Chen, Jen Kun; Shieh, Yi‐Shing; Cochran, David L.; Huang, Ren‐Yeong

doi:10.1016/j.jff.2016.01.025

Cited by 17 publications

(28 citation statements)

References 40 publications

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“…Periodontitis is a chronic inflammatory disease that involves host immune response and is facilitated by inflammatory mediators and oxidative stress. The use of dietary polyphenols having antioxidant and anti‐inflammatory activity as therapeutic strategies for periodontitis is of considerable interest . Genistein is an attractive biomaterial that increases bone mass and improves bone quality .…”

Section: Discussionmentioning

confidence: 99%

Anti‐inflammatory, anti‐osteoclastic, and antioxidant activities of genistein protect against alveolar bone loss and periodontal tissue degradation in a mouse model of periodontitis

Bhattarai

Poudel

Kook

et al. 2017

J Biomedical Materials Res

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Genistein, a dietary polyphenol primarily found in soy products, has beneficial effects on bone. However, the effect of genistein on inflammatory periodontal destruction has not been investigated in detail. We explored whether genistein protects against lipopolysaccharide (LPS)/ligature-induced periodontitis in mice. We also examined the effect of genistein on LPS-stimulated inflammatory and oxidative stress using RAW 264.7 macrophages and human gingival fibroblasts (hGFs). The results from μCT and histological analyses revealed that intraperitoneal injection of genistein (20 mg/kg body weight) daily for three weeks inhibited LPS-mediated alveolar bone loss and periodontal tissue degradation. The administration of genistein also inhibited osteoclast formation and the expression of inflammation-related molecules in the inflamed region of mice with periodontitis. Treatment with 30-70 μM genistein significantly prevented osteoclast differentiation in receptor activator of nuclear factor κB ligand- or LPS-stimulated macrophages by suppressing the expression of osteoclast-specific molecules. The addition of genistein led to a dose-dependent inhibition of the expression of inflammation-related molecules both in LPS-stimulated macrophages and hGFs. In addition, genistein at 50 μM protected hGFs from LPS-mediated stresses such as mitochondrial impairment and cellular ROS accumulation. However, such protection was significantly diminished by combined treatment with 25 nM bafilomycin A1, a chemical autophagy inhibitor. Collectively, our results indicate that genistein protects against inflammatory periodontal damage by regulating autophagy induction and inhibiting osteoclast activation, the production of inflammation mediators, and mitochondrial oxidative damage. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2510-2521, 2017.

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Section: Discussionmentioning

confidence: 99%

Anti‐inflammatory, anti‐osteoclastic, and antioxidant activities of genistein protect against alveolar bone loss and periodontal tissue degradation in a mouse model of periodontitis

Bhattarai

Poudel

Kook

et al. 2017

J Biomedical Materials Res

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“…It also reduced the expression of genes such as dendritic cell-specific transmembrane protein (DC-STAMP), cathepsin K, and matrix metalloproteinase-9 (MMP-9), which is specific to osteoclast differentiation. In addition, it decreased the number of TRAP-positive multinucleated cells with three or more nuclei and the formation of actin rings [72,73].…”

Section: Curcumin’s Effects On Osteogenic Differentiationmentioning

confidence: 99%

“…In case of osteogenic differentiation, curcumin with varying concentrations up to 20 µM shows an inhibition of osteoclast differentiation in a dose-dependent manner, while the optimal dose for the inhibition of osteoclast differentiation was found to be 10 µM [48,72,73,74]. This optimum curcumin dosage is also reported to up-regulate HO-1 expression, induce ER stress markers, and finally, induce osteoblast differentiation [47,50].…”

Section: Effect Of Curcumin Dose On Mesodermal Differentiation Of mentioning

confidence: 99%

“…In an experimental periodontitis study, curcumin was evaluated for the inhibition of osteoclast differentiation, reduction of alveolar bone loss, and periodontal destruction. In this in vivo study, two doses of curcumin—50 mg/kg/day and 30 mg/kg/day—exerted protective effects on alveolar bone loss [72]. In the case of chondrogenic differentiation, 0–5 µM curcumin was reported to suppress IL-1β-induced apoptosis in a concentration-dependent manner [83].…”

Section: Effect Of Curcumin Dose On Mesodermal Differentiation Of mentioning

confidence: 99%

“…* p 0.05, ** p 0.01, *** p 0.001 (Student’s t-test), different from values after treatment with RANKL alone. Adopted with permission from Mau et al with license number 4595960699160 [72].…”

Section: Figurementioning

confidence: 99%

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The Effect of Curcumin on the Differentiation of Mesenchymal Stem Cells into Mesodermal Lineage

et al. 2019

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Curcumin has been placed at the forefront of the researcher’s attention due to its pleiotropic pharmacological effects and health benefits. A considerable volume of articles has pointed out curcumin’s effects on the fate of stem cell differentiation. In this review, a descriptive mechanism of how curcumin affects the outcome of the differentiation of mesenchymal stem cells (MSCs) into the mesodermal lineage—i.e., adipocyte, osteocyte, and chondrocyte differentiation—is compiled from the literature. The sections include the mechanism of inhibition or induction of MSCs differentiation to each lineage, their governing molecular mechanisms, and their signal transduction pathways. The effect of different curcumin doses and its structural modifications on the MSCs differentiation is also discussed.

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Silibinin alleviates inflammation‐induced bone loss by modulating biological interaction between human gingival fibroblasts and monocytes

Huang

Chang

Chih

et al. 2023

Journal of Periodontology

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Background: Silibinin has shown various pharmacological effects that could be attributed to its antioxidant, anti-inflammatory, and immunoregulatory properties. However, the therapeutic potential of silibinin for periodontitis has not been investigated. Methods:The therapeutic effects of silibinin in ligation-induced experimental periodontitis were investigated using biochemical, histological, and immunohistochemical methods. The effects of silibinin on the osteoclastogenesis of RAW264.7 cells were investigated using TRAP staining, quantitative polymerase chain reaction (qPCR), pit formation, and immunoblotting. Moreover, its effects on inflammatory cytokine production, RANKL expression, and oxidative stress in lipopolysaccharide (LPS)-stimulated human gingival fibroblasts (HGFs) were evaluated using qPCR and flow cytometry. A coculture system was established to elucidate the effects of silibinin on the crosstalk between LPS-stimulated HGFs and undifferentiated monocytes. Results: Silibinin significantly reduced the alveolar bone loss, decreased the gingival inflammation and RANKL expression, and decreased the RANKL/ osteoprotegerin ratio in gingival tissues in experimental periodontitis. The in vitro results showed that silibinin inhibited RANKL-induced osteoclast differentiation and function of RAW264.7 cells and suppressed RANKL-induced nuclear factor of activated T cells 1 (NFATc1) induction and translocation through the

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Curcumin ameliorates alveolar bone destruction of experimental periodontitis by modulating osteoclast differentiation, activation and function

Cited by 17 publications

References 40 publications

Anti‐inflammatory, anti‐osteoclastic, and antioxidant activities of genistein protect against alveolar bone loss and periodontal tissue degradation in a mouse model of periodontitis

Anti‐inflammatory, anti‐osteoclastic, and antioxidant activities of genistein protect against alveolar bone loss and periodontal tissue degradation in a mouse model of periodontitis

The Effect of Curcumin on the Differentiation of Mesenchymal Stem Cells into Mesodermal Lineage

Silibinin alleviates inflammation‐induced bone loss by modulating biological interaction between human gingival fibroblasts and monocytes

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