Curcumin alleviates lung injury in diabetic rats by inhibiting nuclear factor‐ κ B pathway

Hafez

et al. 2018

Endocrine Regulations

Objective. We aimed to assess the protective role of verapamil, L-type calcium channel blockers, against early lung damage in diabetic rats. Lung injury has recently been recognized as a consequent complication of diabetes mellitus. Hyperglycemia induces inflammatory changes in lung tissue early in the disease. Methods. Twenty four adult male rats were grouped into control, diabetic, diabetic treated with verapamil, and verapamil control. Streptozotocin (STZ) was used to induce diabetes. Oxidative parameters and antioxidative mechanisms were assessed in lung homogenate. Tumor necrosis factor alpha (TNFα) protein was measured as a pro-inflammatory mediator. Signal transducer and activator of transcription 3 (STAT3) gene expression and nuclear erythroid factor 2 (Nrf2) immunoexpression were screened. Results. The lung showed oxidative damage and inflammatory infiltration in STZ diabetic rats early at 2 weeks. The parameters significantly improved in lung tissue treated with verapamil. Histopathology of the lung tissue confirmed the results. Inhibition of STAT3/TNFα pathway was involved in the protection offered by verapamil. Activation of Nrf2 together with an increasing antioxidant capacity of diabetic lung significantly ameliorates the injury induced by diabetes. Conclusions. Verapamil afforded protection in diabetic lung injury. The protection was mediated by the anti-inflammatory and antioxidant effects of verapamil.

Section: Discussionsupporting

confidence: 63%

STAT3 and Nrf2 pathways modulate the protective effect of verapamil on lung injury of diabetic rats

Hafez

et al. 2018

Endocrine Regulations

“…Zhang et al [43] verified that curcumin could inhibit activation of NF-κB, which has an important role in the inflammatory response, in the lungs of rats with diabetes, thereby alleviating experimentally induced lung inflammatory injury. In a study of peripheral blood mononuclear cells from patients with rheumatoid arthritis, Wei et al [44] found that curcumin could inhibit the osteoclastogenic potential of these cells via suppression of the MAPK/RANK/c-Fos/NFATc1 signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Curcumin Inhibits Polyethylene-Induced Osteolysis via Repressing NF-κB Signaling Pathway Activation

An¹,

Han²,

Hu³

et al. 2018

Cell Physiol Biochem

Background/Aims: Aseptic loosening is a common reason for failed artificial hip replacement after total hip arthroplasty. Aseptic loosening is mostly the result of wear debris that causes osteolysis and weakens the structures that support the prosthesis. Wear debris plays a crucial role in osteolysis during the loosening process, and polyethylene (PE) particles are found as wear debris more frequently than any other type of particle. In the absence of effective therapeutic agents, osteolysis has been hard to treat. Previous studies have demonstrated that curcumin influences signalosome-associated kinases and the proteasome-ubiquitin system during osteoclastogenesis. The aims of this study were to explore the anti-osteolysis effect of curcumin and if possible to identify the signaling pathway involved in a model of PE-induced osteolysis. Methods: Differentiation of osteoclasts was induced in vitro by PE particles in RAW264.7 (monocyte/macrophage) cells and in vivo by calvarial and air pouch models of osteolysis established by PE stimulation in mice. We performed a set of TRAP staining, realtime polymerase chain reaction (PCR), and Western blot experiments to evaluate the anti-osteolytic effect of curcumin by comparing specimens that were exposed and not exposed to curcumin. Results: Curcumin had a promising inhibitory effect on osteolysis induced by wear debris and suppressed the RANK/c-Fos/NFATc1 signaling pathway. Conclusion: Curcumin can prevent PE-induced osteolysis and bone loss. An inhibitory effect on the RANK/c-Fos/NFATc1 signaling pathway may explain the anti-osteolysis activity of curcumin.

Molecular Medicine Reports

“…Harpagophytum procumbens (devil's claw) suppresses the inflammatory reaction of LPS-stimulated human monocytes by inhibiting the release of cytokines and PGE 2, and the expression of COX-2, IL-6 and TNF-α mRNA, without affecting the NF-κB and MAPK signaling pathways (24). Curcumin reduces LPS-stimulated inflammatory responses (NO, PGE 2 and cytokine production) in the lungs of diabetic rats by suppressing NF-κB activation (25). Thus, all the functional food materials described above are unlikely to exhibit anti-inflammatory actions on IL-1β-induced production of IL-8 by synovial cells evaluated in the present study, although they suppress the inflammatory reactions assessed under other experimental conditions.…”

Section: Discussionmentioning

confidence: 99%

“…IL-1β was purchased from Peprotech EC Ltd. (London, UK). In addition, the effects of the following substances, which are known to exhibit anti-inflammatory or anti-arthritic action (17)(18)(19)(20)(21)(22)(23)(24)(25), were evaluated: Proteoglycan extracted from salmon nasal cartilage (17) was supplied by Ichimaru Pharcos Co., Ltd. (Gifu, Japan), hyaluronic acid (18) by N.C. Corporation (Kagawa, Japan), collagen peptides (19,20) by Nitta Gelatin Inc. (Osaka, Japan), olive leaf extract (21) by Eisai Food & Chemical Co., Ltd. (Tokyo, Japan), red ginger extract (22) from Oryza Oil & Fat Chemical Co., Ltd. (Aichi, Japan), Boswellia serrata extract (23) from Nippon Shinyaku Co., Ltd., devil's claw extract (24) from Ask Intercity Co., Ltd. (Chiba, Japan) and curcumin (25) from Indena Japan Co., Ltd. (Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the anti-inflammatory actions of various functional food materials including glucosamine on synovial cells

Yamagishi

Someya

Imai

et al. 2017

The anti-inflammatory actions of glucosamine (GlcN) on arthritic disorders involve the suppression of inflammatory mediator production from synovial cells. GlcN has also been reported to inhibit the activation of the p38 mitogen-activated protein kinase (MAPK) pathway. The present study aimed to determine the cooperative and anti‑inflammatory actions of functional food materials and evaluated the production of interleukin (IL)‑8 and phosphorylation of p38 MAPK in IL-1β-activated synovial cells, incubated with the combination of GlcN and various functional food materials containing L‑methionine (Met), undenatured type II collagen (UC‑II), chondroitin sulfate (CS), methylsulfonylmethane (MSM) and agaro-oligosaccharide (AO). The results indicated that Met, UC‑II, CS, MSM and AO slightly or moderately suppressed the IL-1β-stimulated IL‑8 production by human synovial MH7A cells. The same compounds further decreased the IL‑8 level lowered by GlcN. Similarly, they slightly suppressed the phosphorylation level of p38 MAPK and further reduced the phosphorylation level lowered by GlcN. These observations suggest a possibility that these functional food materials exert an anti‑inflammatory action (inhibition of IL‑8 production) in combination with GlcN by cooperatively suppressing the p38 MAPK signaling (phosphorylation).