Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway

Abstract

Lipopolysaccharide (LPS) is an endotoxin, which may cause immune response and inflammation of bovine mammary glands. Mastitis impairs animal health and results in economic loss. Curcumin (CUR) is a naturally occurring diketone compound, which has attracted widespread attention as a potential anti-inflammatory antioxidant. The purpose of this study is to investigate whether CUR can reduce the damage of bovine mammary epithelial cells (MAC-T) induced by LPS and its underlying molecular mechanism. The MAC-T cell line was treated with different concentrations of LPS and CUR for 24 h. The results showed that CUR rescued the decrease of MAC-T cell viability and cell damage induced by LPS. At the same time, 10 µM CUR and 100 µg/mL LPS were used to treat the cells in the follow-up study. The results showed CUR treatment reduced the accumulation of reactive oxygen species (ROS), the expression of inflammatory cytokines (tumor necrosis factor-a (TNF-α), interleukin-8 (IL-8), IL-6 and IL-1β) and the rate of apoptosis induced by LPS. These effects were associated with the activation of the nuclear factor E2-related factor 2 (NFE2L2)-antioxidant response element (ARE) pathway coupled with inactivation of the nuclear factor-κB (NF-κB) inflammatory and caspase/Bcl2 apoptotic pathways.

N6-Methyladenosine Modification Profile in Bovine Mammary Epithelial Cells Treated with Heat-Inactivated Staphylococcus aureus

“…On the other hand, ROS can cause oxidation of proteins and DNA, inducing damage to nearby tissues. In this study, we used inactivated S. aureus to stimulate MAC-T cells, and we found that the expression levels of the inflammatory factors and ROS increased significantly ( Figure 1 ), consistent with the results of other related studies [ 47 , 48 ].…”

Curcumin, an Inhibitor of p300-HAT Activity, Suppresses the Development of Hypertension-Induced Left Ventricular Hypertrophy with Preserved Ejection Fraction in Dahl Rats

“…Curcumin (diferuloyl-methane) is a natural yellow compound isolated from the rhizome of Curcuma longa . Many reports demonstrate that curcumin shows different kinds of beneficial effects, including antioxidant [ 16 ], anti-inflammatory [ 17 ] and antitumor effects [ 18 , 19 ], while also inhibiting the p300/CBP-specific HAT activity [ 20 ]. Even though curcumin has low bioavailability, highly bioavailable forms of curcumin is expected to exhibit potential health benefits and expected to contribute to the health management [ 21 , 22 ].…”

Identification and Validation of Ferroptosis-Related Biomarkers in Septic Cardiomyopathy via Bioinformatics Analysis

“…Moreover, Nfe2l2 (Nuclear Factor, Erythroid 2 Like 2) is a Protein Coding gene. Li et al reported that Curcumin alleviates LPS-induced oxidative stress, in bovine mammary epithelial cells via the Nfe2l2 signaling pathway ( Li et al, 2021 ). However, the role of Nfe2l2 in LPS-induced myocardial injury has not been studied.…”