Curcumin: a potential exogenous additive for the prevention of LPS‐induced duck ileitis by the alleviation of inflammation and oxidative stress

Abstract

BACKGROUND Lipopolysaccharides (LPS) are the main pathogenic substances in Gram‐negative bacteria. The aim of this study was to investigate the preventive effects of dietary curcumin (CUR) on LPS toxicity in the duck ileum. The duck diet was supplemented with CUR (0.5 g kg−1) for 28 days, while the birds were injected with LPS (0.5 mg kg−1 body weight per injection, administered as seven injections in the last week of the experimental period). RESULTS LPS significantly decreased the ileal villus‐to‐crypt ratio in the non‐supplemented CUR group. Dietary CUR alleviated LPS‐induced morphological damage to the ileum. Moreover, dietary CUR alleviated oxidative stress by increasing the levels of total superoxide dismutase (T‐SOD) (P < 0.05) and glutathione S‐transferase (GST) (P < 0.05) and decreasing the production of malonic dialdehyde (MDA) (P < 0.05) in control ducks and LPS‐challenged ducks. Dietary CUR significantly inhibited the LPS‐induced massive production of inflammatory factors (IL‐1β, IL‐6, and TNF‐α) (P < 0.05). CUR induced the inhibition of TLR4 and activation of Nrf2 to reduce the expression of inflammation‐related genes (TLR4, NF‐κB, IKK, TXNIP, NLRP3, caspase‐1, IL‐1β, IL‐6, and TNF‐α). Moreover, dietary CUR ameliorated the decrease in claudin‐1 and occludin expression (P < 0.05) and improved ZO‐1 expression in the duck ileum (P < 0.05). CONCLUSION In conclusion, dietary CUR has beneficial effects on LPS‐induced ileal damage, oxidative damage, and inflammatory response by inhibiting the TLR/NF‐κB and activating the Nrf2 signaling pathways in ducks. This study provides valuable information regarding the therapeutic uses of CUR in duck ileitis. © 2022 Society of Chemical Industry.

A Novel Polysaccharide Separated from Panax Notoginseng Residue Ameliorates Restraint Stress‐ and Lipopolysaccharide‐induced Enteritis in Mice

“…In addition, the downregulation of Toll‐like receptor (TLR)/NLRP3/inducible nitric oxide synthase pathway is an innovative and safe strategy for treating patients with IBD [30] . Inhibition of TLR4, one LPS target, can reduce the expression of inflammatory factors including NF‐κB, NLRP3 and TNF‐α [31] . Previous data show that the molecular weight of polysaccharide ligands of TLR4 receptor is between 10~1000 kDa, which has high biological activities [32] .…”

“…[30] Inhibition of TLR4, one LPS target, can reduce the expression of inflammatory factors including NF-kB, NLRP3 and TNF-α. [31] Previous data show that the molecular weight of polysaccharide ligands of TLR4 receptor is between 10~1000 kDa, which has high biological activities. [32] Current data indicated that the average molecular weight of PNP-20, -40, and -60 was less than 1000 kDa.…”

Curcumin as a Potential Antioxidant in Stress Regulation of Terrestrial, Avian, and Aquatic Animals: A Review

“…On the other hand, lipopolysaccharides (LPSs), components responsible for the pathological process of contamination induced by Gram-negative bacteria, can cause morphological damage to the ileum, lung injury, inflammation, and oxidative stress in poultry. The addition of 500 mg/kg of curcumin to the diet could ameliorate these symptoms in the lungs and ileitis of ducks through the signaling pathways of Nrf2-ARE, NF-κB, and TLR/NF-κB in an orderly manner [ 87 , 88 ]. Furthermore, curcumin has shown effectiveness against coccidiosis caused by Eimeria species.…”

A highly effective “naked eye” colorimetric and fluorimetric curcumin-based fluorescent sensor for specific and sensitive detection of H2O2in vivo and in vitro

“…We propose that the curcumin-based fluorescent sensor may have low cytotoxicity. [33][34][35] Moreover, curcumin and its derivatives have a large conjugate structure, which could enhance the π electron delocalization. The curcumin and its derivatives are better to be emitted to generate outstanding long-wavelength fluorescence.…”