Curcuma purpurascens BI. rhizome accelerates rat excisional wound healing: involvement of Hsp70/Bax proteins, antioxidant defense, and angiogenesis activity

Abstract

PurposeCurcuma purpurascens BI. is a member of Zingiberaceae family. The purpose of this study is to investigate the wound healing properties of hexane extract of C. purpurascens rhizome (HECP) against excisional wound healing in rats.Materials and methodsTwenty four rats were randomly divided into 4 groups: A) negative control (blank placebo, acacia gum), B) low dose of HECP, C) high dose of HECP, and D) positive control, with 6 rats in each group. Full-thickness incisions (approximately 2.00 cm) were made on the neck area of each rat. Groups 1–4 were treated two-times a day for 20 days with blank placebo, HECP (100 mg/kg), HECP (200 mg/kg), and intrasite gel as a positive control, respectively. After 20 days, hematoxylin and eosin and Masson’s trichrome stainings were employed to investigate the histopathological alterations. Protein expressions of Bax and Hsp70 were examined in the wound tissues using immunohistochemistry analysis. In addition, levels of enzymatic antioxidants and malondialdehyde representing lipid peroxidation were measured in wound tissue homogenates.ResultsMacroscopic evaluation of wounds showed conspicuous elevation in wound contraction after topical administration of HECP at both doses. Moreover, histopathological analysis revealed noteworthy reduction in the scar width correlated with the enhanced collagen content and fibroblast cells, accompanied by a reduction of inflammatory cells in the granulation tissues. At the molecular level, HECP facilitates wound-healing process by downregulating Bax and upregulating Hsp70 protein at the wound site. The formation of new blood vessel was observed in Masson’s trichrome staining of wounds treated with HECP (100 and 200 mg/kg). In addition, HECP administration caused a significant surge in enzymatic antioxidant activities and a decline in lipid peroxidation.ConclusionThese findings suggested that HECP accelerated wound-healing process in rats via antioxidant activity, angiogenesis effect and anti-inflammatory responses involving Hsp70/Bax.

Chemical Characterization and Wound Healing Property of Jacaranda decurrens Cham. (Bignoniaceae): An Experimental Study Based on Molecular Mechanisms

“…(FtEHJ) promoted a wound healing evolution homogeneously, with controlled inflammatory process and in a shorter time compared to other groups. On the first day after lesion induction, FtEHJ [27]. Bft � basis of the topical formulation; FtEHJ � Topical formulation of the hydroalcoholic extract of J. decurrens Cham.…”

“…Evidence-Based Complementary and Alternative Medicine and collagenization. Specimens were examined under a light microscope (100x and 400x) and scored accordingly: absent; discrete (up to 25% of the parameter); moderate (25-50%); and marked (50-100%) [25,27].…”

<i>In silico</i> and <i>in vivo</i> anti-inflammatory studies of curcuminoids, turmeric extract with zinc oxide, and eugenol

“…The study of the group treated with zinc oxide with turmeric rhizome liquid extract showed a TNFα expression lower than that of the other groups on day 3 (A3) which decreased further on day 7 (B3), indicating that the inflammatory process had subsided.. This proved that the curcuminoids from zinc oxide dressing with turmeric extract effectively counteract oxidant and free radicals which are detached from HSP 70 due to excision trauma [13]. These results are consistent with studies showing an increase in macrophage phagocyte activity in animal subjects given curcumin.…”

Medicinal Plants and Natural Products with Demonstrated Wound Healing Properties

“…At the molecular level, C. purpurascens facilitated wound-healing process by down-regulating Bax and up-regulating Hsp70 protein at the wound site. In addition, the plant enhanced catalase, glutathione peroxidase, superoxide dismutase activity and reduced malondialdehyde levels in wound tissues compared to the control group [184].…”