Cupuaçu extract reduces nitrosative stress and modulates inflammatory mediators in the kidneys of experimental diabetes

Punaro, Giovana Rita; Lima, Deyse Y.; Rodrigues, Adelson Marçal; Pugliero, Samuel; Mouro, Margaret Gori; Rogero, Marcelo M.; Higa, Elisa M S

doi:10.1016/j.clnu.2017.12.016

“…However, the seeds are highly rich in proteins, minerals, bioactive compounds, and fat, presenting a varied content of fatty acids (±60% dry weight) with characteristics and potential to Pharmaceutics 2022, 14, 207 2 of 16 be applied in cosmetics and pharmaceutical products [3]. Despite few pieces of research attempting to employ the fat from the Cupuaçu seeds in other food products as a substitute for cocoa butter [4], investigations on the biotechnological and therapeutic potentialities of this exotic fruit and its extracts have been limited to applications related to their antioxidant properties [5,6].…”

Section: Introductionmentioning

confidence: 99%

Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules

Barbalho

¹

,

Matos

²

,

Brito

³

et al. 2022

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Scarless skin regeneration is a challenge in regenerative medicine. Herein, we explore the regenerative potential of a Cupuaçu seed extract (Theobroma grandiflorum) to develop an innovative skin regeneration formulation based on chitosan-coated nanocapsules. Cupuaçu seed extract significantly stimulated cell proliferation and migration. A reparative gene expression profile could be verified following extract treatment, which included high levels of MKI67, a cellular proliferation marker, and extracellular matrix genes, such as ELN and HAS2, which code for elastin and hyaluronic acid synthase 2. Formulations with Cupuaçu seed extract successfully entrapped into nanocapsules (EE% > 94%) were developed. Uncoated or coated nanocapsules with low-molecular-weight chitosan presented unimodal size distribution with hydrodynamic diameters of 278.3 ± 5.0 nm (PDI = 0.18 ± 0.02) and 337.2 ± 2.1 nm (PDI = 0.27 ± 0.01), respectively. Both nanosystems were physically stable for at least 120 days and showed to be non-irritating to reconstructed human epidermis. Chitosan coating promoted active penetration into undamaged skin areas, which were still covered by the stratum corneum. In conclusion, the present study demonstrated for the first time the biotechnological potential of the frequently discarded Cupuaçu seed as a valuable pharmaceutical ingredient to be used in regenerative skin products.

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“…Oxidative stress is a critical factor in the pathophysiology of DN and is one of the main mechanisms of this disease [11]. A previous study in our Laboratory showed that antioxidants such as cupuaçu had a bene cial effect on diabetic models [12]. Thus, the use of antioxidants as a therapeutic strategy could alleviate cellular damage in DM.…”

Section: Introductionmentioning

confidence: 96%

“…Experimental models of diabetes induced in rats have been widely used by researchers worldwide because animal models have clinical, laboratory and histopathological similarities with human diabetes [13]. Several studies have shown that changes in diet and rich foods in antioxidants, tested in animal models, can improve kidney function and clinical markers related to diabetes and in ammation [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Açai (Euterpe oleracea Mart) modulates oxidative stress and inflammation by NF-κB inactivation and Nrf2 up-regulation in experimental diabetes

Lima

¹

,

Rodrigues

²

,

Mouro

³

et al. 2022

Preprint

0

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To evaluate the effects of açai extract (EA) on oxidative stress and inflammation induced by high glucose in cultured mouse immortalized mesangial cells (MiMC) and diabetic rats. MiMC cell viability and proliferation were determined by MTT. Extracellular and intracellular nitric oxide (NO) and intracellular ROS were also measured. The cell proteins were extracted for analysis of catalase, Nrf2, p-Nrf2, SOD-1, SOD-2, iNOS, NF-κB, p-NF-κB and TNF-α expression. Male, adult Wistar rats were distributed into 3 groups: control (CTL) and diabetic (DM) rats who received water and DMEA and received 1 mL/day EA (200 mg/kg) via gavage for 8 consecutive weeks. After treatment with EA, metabolic profile, renal function and thiobarbituric acid reactives substances (TBARS) levels were evaluated, and kidneys were collected for qualitative histological analysis. EA maintained cell viability above 90% in all groups; it decreased proliferation in the HG group, both significant. NO levels, ROS generation, iNOS, NF-κB, p-NF-κB and TNF-α expression were reduced significantly after 72 h of EA treatment, with significant increases for all antioxidants studied. DMEA vs DM showed a significant increase in body weight, improved kidney function and reduced TBARS excretion. EA treatment decreased proliferation, oxidative stress and inflammation in MiMC, and although açai did not decrease fasting glucose, it recovered the body weight and delayed the decline of renal function in the diabetic animals, suppressing the signaling of inflammatory mediators via NF-κB inactivation and increasing all antioxidants studied by upregulating the Nrf2 response pathway.

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“…Experimental models of diabetes induced in rats have been widely used by researchers worldwide because animal models have clinical, laboratory and histopathological similarities with human diabetes [13]. Several studies have shown that changes in diet and rich foods in antioxidants, tested in animal models, can improve kidney function and clinical markers related to diabetes and inflammation [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Oxidative stress is a critical factor in the pathophysiology of DN and is one of the main mechanisms of this disease [11]. A previous study in our Laboratory showed that antioxidants such as cupuaçu had a beneficial effect on diabetic models [12]. Thus, the use of antioxidants as a therapeutic strategy could alleviate cellular damage in DM.…”

Section: Introductionmentioning

confidence: 99%

Açai (Euterpe oleracea Mart) modulates oxidative stress and inflammation by NF-κB inactivation and Nrf2 up-regulation in experimental diabetes

Lima

¹

,

Rodrigues

²

,

Mouro

³

et al. 2022

Preprint

1

0

View full text Add to dashboard Cite

To evaluate the effects of açai extract (EA) on oxidative stress and inflammation induced by high glucose in cultured mouse immortalized mesangial cells (MiMC) and diabetic rats. MiMC cell viability and proliferation were determined by MTT. Extracellular and intracellular nitric oxide (NO) and intracellular ROS were also measured. The cell proteins were extracted for analysis of catalase, Nrf2, p-Nrf2, SOD-1, SOD-2, iNOS, NF-κB, p-NF-κB and TNF-α expression. Male, adult Wistar rats were distributed into 3 groups: control (CTL) and diabetic (DM) rats who received water and DMEA and received 1 mL/day EA (200 mg/kg) via gavage for 8 consecutive weeks. After treatment with EA, metabolic profile, renal function and thiobarbituric acid reactives substances (TBARS) levels were evaluated, and kidneys were collected for qualitative histological analysis. EA maintained cell viability above 90% in all groups; it decreased proliferation in the HG group, both significant. NO levels, ROS generation, iNOS, NF-κB, p-NF-κB and TNF-α expression were reduced significantly after 72 h of EA treatment, with significant increases for all antioxidants studied. DMEA vs DM showed a significant increase in body weight, improved kidney function and reduced TBARS excretion. EA treatment decreased proliferation, oxidative stress and inflammation in MiMC, and although açai did not decrease fasting glucose, it recovered the body weight and delayed the decline of renal function in the diabetic animals, suppressing the signaling of inflammatory mediators via NF-κB inactivation and increasing all antioxidants studied by upregulating the Nrf2 response pathway.

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Cupuaçu extract reduces nitrosative stress and modulates inflammatory mediators in the kidneys of experimental diabetes

Cited by 22 publications

References 35 publications

Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules

Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules

Açai (Euterpe oleracea Mart) modulates oxidative stress and inflammation by NF-κB inactivation and Nrf2 up-regulation in experimental diabetes

Açai (Euterpe oleracea Mart) modulates oxidative stress and inflammation by NF-κB inactivation and Nrf2 up-regulation in experimental diabetes

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